HAMAP rule MF_00830
General rule information
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| Accession | MF_00830 |
| Dates | 2-NOV-2010 (Created) 1-JUN-2023 (Last updated, Version 12) |
| Name | RutB |
| Scope | Bacteria |
| Template | P75897 (RUTB_ECOLI) |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Hydrolyzes ureidoacrylate to form aminoacrylate and carbamate. The carbamate hydrolyzes spontaneously, thereby releasing one of the nitrogen atoms of the pyrimidine ring as ammonia and one of its carbon atoms as CO2. |
| Catalytic activity | RHEA:42624: (Z)-3-ureidoacrylate + H(+) + H2O = (Z)-3-aminoacrylate + CO2 + NH4(+)
EC 3.5.1.110 |
| RHEA:31603: (Z)-3-ureidoacrylate + H2O = (Z)-3-aminoacrylate + carbamate + H(+) | |
| RHEA:42620: (Z)-2-methylureidoacrylate + H(+) + H2O = (Z)-2-methylaminoacrylate + CO2 + NH4(+)
EC 3.5.1.110 |
case <OC:Escherichia> or <OC:Shigella>
| Induction | Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR. |
end case
| Similarity | Belongs to the isochorismatase family. RutB subfamily. |
Keywords
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Gene Ontology
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GO:0016811; Molecular function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides.
GO:0006212; Biological process: uracil catabolic process.
GO:0019740; Biological process: nitrogen utilization.
GO:0006212; Biological process: uracil catabolic process.
GO:0019740; Biological process: nitrogen utilization.
Cross-references
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Features
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| From: RUTB_ECOLI (P75897) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 24 | 24 | Proton acceptor | D | ||||||||
| ACT_SITE | 133 | 133 | K | |||||||||
| ACT_SITE | 166 | 166 | Nucleophile | C | ||||||||
Additional information
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| Size range | 225-258 amino acids |
| Related rules | None |
| Fusion | None |