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Annotation rule MF_00834
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General rule information [?]

Accession MF_00834
Dates 22-JUN-2011 (Created)
17-APR-2020 (Last updated, Version 10)
Name BioA
Templates P12995 (BIOA_ECOLI); P9WQ81 (BIOA_MYCTU); P53555 (BIOK_BACSU); P22805 (BIOA_LYSSH): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Adenosylmethionine-8-amino-7-oxononanoate aminotransferase;
AltName: Full=7,8-diamino-pelargonic acid aminotransferase;
Short=DAPA AT;
Short=DAPA aminotransferase;
AltName: Full=7,8-diaminononanoate synthase;
AltName: Full=Diaminopelargonic acid synthase;
Gene name

Comments [?]

Function Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.
Catalytic activity RHEA:16861: (S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = (7R,8S)-7,8-diammoniononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate
Cofactor pyridoxal 5'-phosphate
Pathway Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.

Keywords [?]

Gene Ontology [?]

GO:0004015; Molecular function: adenosylmethionine-8-amino-7-oxononanoate transaminase activity.
GO:0030170; Molecular function: pyridoxal phosphate binding.
GO:0009102; Biological process: biotin biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00202; Aminotran_3; 1;
TIGRFAMs TIGR00508; BioA; 1;

Features [?]

From: BIOA_ECOLI (P12995)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     112     113       Pyridoxal phosphate binding     G-[SA]  
REGION     308     309       Pyridoxal phosphate binding     [PHNS]-[TS]  
BINDING     52     52       Substrate     W  
BINDING     144     144       Substrate     Y  
BINDING     245     245       Pyridoxal phosphate     D  
BINDING     274     274       Substrate     K  
BINDING     307     307       Substrate; via carbonyl oxygen     [GS]  
BINDING     391     391       Substrate     R  
MOD_RES     274     274       N6-(pyridoxal phosphate)lysine     K  
SITE     17     17       Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM        

Additional information [?]

Size range 400-560 amino acids
Related rules None
Fusion Nter: MF_01694 (BioB); Cter: None
Comments B.subtilis DAPA aminotransferase does not utilize SAM, but instead utilizes L-lysine as the amino donor (PMID:15880481). Bacteroides encode a fusion between BioA and BioB (Biotin synthase).