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HAMAP rule MF_00834

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General rule information [?]

Accession MF_00834
Dates 22-JUN-2011 (Created)
1-JUN-2023 (Last updated, Version 15)
Name BioA
Scope(s) Bacteria
Template(s) P12995 (BIOA_ECOLI); P9WQ81 (BIOA_MYCTU); P53555 (BIOK_BACSU); P22805 (BIOA_LYSSH); [ Recover all ]
Triggered by HAMAP; MF_00834 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier BIOA
Protein name RecName: Full=Adenosylmethionine-8-amino-7-oxononanoate aminotransferase;
AltName: Full=7,8-diamino-pelargonic acid aminotransferase;
                 Short=DAPA AT;
                 Short=DAPA aminotransferase;
AltName: Full=7,8-diaminononanoate synthase;
AltName: Full=Diaminopelargonic acid synthase;
Gene name Name=bioA;

Comments [?]

FUNCTIONCatalyzes the transfer of the alpha-amino group from S- adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.
CATALYTIC ACTIVITY Reaction=(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = (7R,8S)-7,8-diammoniononanoate + S-adenosyl-4-methylsulfanyl-2- oxobutanoate; Xref=Rhea:RHEA:16861, ChEBI:CHEBI:16490, ChEBI:CHEBI:59789, ChEBI:CHEBI:149468, ChEBI:CHEBI:149469; EC=;
COFACTOR Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
PATHWAYCofactor biosynthesis; biotin biosynthesis; 7,8- diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1.
SIMILARITYBelongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.

Keywords [?]

Gene Ontology [?]

GO:0004015; Molecular function:adenosylmethionine-8-amino-7-oxononanoate transaminase activity
GO:0030170; Molecular function:pyridoxal phosphate binding
GO:0009102; Biological process:biotin biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00202; Aminotran_3; 1;
NCBIfam TIGR00508; BioA; 1;

Features [?]

From: BIOA_ECOLI (P12995)
Key From To Description Tag Condition FTGroup
BINDING 112 113 /ligand="pyridoxal 5'-phosphate"
BINDING 308 309 /ligand="pyridoxal 5'-phosphate"
BINDING 52 52 /ligand="substrate" W
BINDING 144 144 /ligand="substrate" Y
BINDING 245 245 /ligand="pyridoxal 5'-phosphate"
BINDING 274 274 /ligand="substrate" K
BINDING 307 307 /ligand="substrate" [GS]
BINDING 391 391 /ligand="substrate" R
MOD_RES 274 274 /note="N6-(pyridoxal phosphate)lysine" K
SITE 17 17 /note="Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM"

Additional information [?]

Size range 400-560 amino acids
Related rules None
Fusion Nter: MF_01694 (BioB) Cter: None
Comments B.subtilis DAPA aminotransferase does not utilize SAM, but instead utilizes L-lysine as the amino donor (PMID:15880481). Bacteroides encode a fusion between BioA and BioB (Biotin synthase).

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