HAMAP rule MF_00834
General rule information
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| Accession | MF_00834 |
| Dates | 22-JUN-2011 (Created)
1-JUN-2023 (Last updated, Version 15) |
| Name | BioA |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P12995 (BIOA_ECOLI); P9WQ81 (BIOA_MYCTU); P53555 (BIOK_BACSU); P22805 (BIOA_LYSSH); [ Recover all ] |
| Triggered by |
HAMAP; MF_00834 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | BIOA |
| Protein name | RecName: Full=Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; EC=2.6.1.62; AltName: Full=7,8-diamino-pelargonic acid aminotransferase; Short=DAPA AT; Short=DAPA aminotransferase; AltName: Full=7,8-diaminononanoate synthase; Short=DANS; AltName: Full=Diaminopelargonic acid synthase; |
| Gene name | Name=bioA; |
Comments
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| FUNCTION | Catalyzes the transfer of the alpha-amino group from S- adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor. |
| CATALYTIC ACTIVITY | Reaction=(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = (7R,8S)-7,8-diammoniononanoate + S-adenosyl-4-methylsulfanyl-2- oxobutanoate; Xref=Rhea:RHEA:16861, ChEBI:CHEBI:16490, ChEBI:CHEBI:59789, ChEBI:CHEBI:149468, ChEBI:CHEBI:149469; EC=2.6.1.62; |
| COFACTOR | Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; |
| PATHWAY | Cofactor biosynthesis; biotin biosynthesis; 7,8- diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1. |
| SUBUNIT | Homodimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily. |
Keywords
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| Aminotransferase |
| Biotin biosynthesis |
| Cytoplasm |
| Pyridoxal phosphate |
| S-adenosyl-L-methionine |
| Transferase |
Gene Ontology
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| GO:0004015; Molecular function:adenosylmethionine-8-amino-7-oxononanoate transaminase activity |
| GO:0030170; Molecular function:pyridoxal phosphate binding |
| GO:0009102; Biological process:biotin biosynthetic process |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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| From: BIOA_ECOLI (P12995) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 112 | 113 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" |
G-[SA] | ||||||||
| BINDING | 308 | 309 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" |
[PHNS]-[TS] | ||||||||
| BINDING | 52 | 52 | /ligand="substrate" | W | ||||||||
| BINDING | 144 | 144 | /ligand="substrate" | Y | ||||||||
| BINDING | 245 | 245 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" |
D | ||||||||
| BINDING | 274 | 274 | /ligand="substrate" | K | ||||||||
| BINDING | 307 | 307 | /ligand="substrate" | [GS] | ||||||||
| BINDING | 391 | 391 | /ligand="substrate" | R | ||||||||
| MOD_RES | 274 | 274 | /note="N6-(pyridoxal phosphate)lysine" | K | ||||||||
| SITE | 17 | 17 | /note="Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM" | |||||||||
Additional information
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| Size range | 400-560 amino acids |
| Related rules |
None |
| Fusion | Nter: MF_01694 (BioB) Cter: None |
| Comments | B.subtilis DAPA aminotransferase does not utilize SAM, but instead utilizes L-lysine as the amino donor (PMID:15880481). Bacteroides encode a fusion between BioA and BioB (Biotin synthase). |