HAMAP rule MF_00845

General rule information [?]

Accession	MF_00845
Dates	6-SEP-2019 (Created) 19-NOV-2022 (Last updated, Version 5)

Name	TetX_monooxygenase

Scope

Bacteria

Templates

Q93L51 (TETX_BACT4); Q01911 (TETX_BACFG): [Recover all]

Triggered by

HAMAP; MF_00845 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

TETX

Protein name

RecName:		Full=Flavin-dependent monooxygenase;
AltName:		Full=TetX monooxygenase;
		Short=TetX;
		EC 1.14.13.-;

Comments [?]

Function	An FAD-requiring monooxygenase active on some tetracycline antibiotic derivatives, which leads to their inactivation. Hydroxylates carbon 11a of tetracycline and some analogs.
Catalytic activity	RHEA:61444: a tetracycline + H(+) + NADPH + O2 = an 11a-hydroxytetracycline + H2O + NADP(+)
Cofactor	FAD
Subunit	Monomer.
Subcellular location	Cytoplasm.
Domain	Consists of an N-terminal FAD-binding domain with a Rossman fold and a C-terminal substrate-binding domain.
Similarity	Belongs to the aromatic-ring hydroxylase family. TetX subfamily.

Keywords [?]

Cytoplasm
FAD
Flavoprotein
Monooxygenase
NADP
Nucleotide-binding
Oxidoreductase

Gene Ontology [?]

GO:0004497; Molecular function: monooxygenase activity.
GO:0000166; Molecular function: nucleotide binding.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF01494; FAD_binding_3; 1;

Features [?]

From: TETX_BACT4 (Q93L51)

Key From To Description Tag Condition FTGroup

BINDING 54 54 /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783 R

BINDING 61 61 /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692 D

BINDING 117 117 /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692 R

BINDING 311 311 /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692 D

Additional information [?]

Size range	357-413 amino acids
Related rules	None
Fusion	None
Comments	Other TetX proteins able to modify tetracycline and its analogs have been isolated from Mycobacterium abscessus and soil bacteria. These proteins are not members of this family. The proteins from soil bacteria modify a different position on the antibiotic and generate a different product.

View rule in raw text format (no links)