HAMAP logo

HAMAP rule MF_00845

Send feedback

General rule information [?]

Accession MF_00845
Dates 6-SEP-2019 (Created)
19-NOV-2022 (Last updated, Version 5)
Name TetX_monooxygenase
Templates Q93L51 (TETX_BACT4); Q01911 (TETX_BACFG): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Flavin-dependent monooxygenase;
AltName: Full=TetX monooxygenase;
EC 1.14.13.-;

Comments [?]

Function An FAD-requiring monooxygenase active on some tetracycline antibiotic derivatives, which leads to their inactivation. Hydroxylates carbon 11a of tetracycline and some analogs.
Catalytic activity RHEA:61444: a tetracycline + H(+) + NADPH + O2 = an 11a-hydroxytetracycline + H2O + NADP(+)
Cofactor FAD
Subunit Monomer.
Subcellular location Cytoplasm.
Domain Consists of an N-terminal FAD-binding domain with a Rossman fold and a C-terminal substrate-binding domain.
Similarity Belongs to the aromatic-ring hydroxylase family. TetX subfamily.

Keywords [?]

Gene Ontology [?]

GO:0004497; Molecular function: monooxygenase activity.
GO:0000166; Molecular function: nucleotide binding.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01494; FAD_binding_3; 1;

Features [?]

From: TETX_BACT4 (Q93L51)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     54     54       /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783     R  
BINDING     61     61       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     D  
BINDING     117     117       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     R  
BINDING     311     311       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     D  

Additional information [?]

Size range 357-413 amino acids
Related rules None
Fusion None
Comments Other TetX proteins able to modify tetracycline and its analogs have been isolated from Mycobacterium abscessus and soil bacteria. These proteins are not members of this family. The proteins from soil bacteria modify a different position on the antibiotic and generate a different product.