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HAMAP rule MF_00845

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General rule information [?]

Accession MF_00845
Dates 6-SEP-2019 (Created)
19-NOV-2022 (Last updated, Version 5)
Name TetX_monooxygenase
Scope(s) Bacteria
Template(s) Q93L51 (TETX_BACT4); Q01911 (TETX_BACFG); [ Recover all ]
Triggered by HAMAP; MF_00845 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TETX
Protein name RecName: Full=Flavin-dependent monooxygenase;
AltName: Full=TetX monooxygenase;

Comments [?]

FUNCTIONAn FAD-requiring monooxygenase active on some tetracycline antibiotic derivatives, which leads to their inactivation. Hydroxylates carbon 11a of tetracycline and some analogs.
CATALYTIC ACTIVITY Reaction=a tetracycline + H(+) + NADPH + O2 = an 11a- hydroxytetracycline + H2O + NADP(+); Xref=Rhea:RHEA:61444, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:144644, ChEBI:CHEBI:144645;
DOMAINConsists of an N-terminal FAD-binding domain with a Rossman fold and a C-terminal substrate-binding domain.
SIMILARITYBelongs to the aromatic-ring hydroxylase family. TetX subfamily.

Keywords [?]

Gene Ontology [?]

GO:0004497; Molecular function:monooxygenase activity
GO:0000166; Molecular function:nucleotide binding
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01494; FAD_binding_3; 1;

Features [?]

From: TETX_BACT4 (Q93L51)
Key From To Description Tag Condition FTGroup
BINDING 54 54 /ligand="NADPH"
BINDING 61 61 /ligand="FAD"
BINDING 117 117 /ligand="FAD"
BINDING 311 311 /ligand="FAD"

Additional information [?]

Size range 357-413 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Other TetX proteins able to modify tetracycline and its analogs have been isolated from Mycobacterium abscessus and soil bacteria. These proteins are not members of this family. The proteins from soil bacteria modify a different position on the antibiotic and generate a different product.

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