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Annotation rule MF_00850 |
General rule information
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Accession |
MF_00850 |
Dates |
23-MAR-2020 (Created) 24-MAR-2020 (Last updated, Version 2) |
Propagated annotation
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Identifier, protein and gene names
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Protein name |
RecName:
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Full=Pyruvate dehydrogenase [ubiquinone];
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EC 1.2.5.1;
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AltName:
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Full=Pyruvate oxidase;
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Short=POX;
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AltName:
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Full=Pyruvate:ubiquinone-8 oxidoreductase;
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Function |
A peripheral cell membrane enzyme that catalyzes the oxidative decarboxylation of pyruvate to form acetate and CO(2). It channels electrons from the cytoplasm to the respiratory chain at the cell membrane via ubiquinone. |
Catalytic activity |
RHEA:27405: a ubiquinone + H2O + pyruvate = a ubiquinol + acetate + CO2
EC 1.2.5.1
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Cofactor |
FAD Note: Binds 1 FAD per subunit. |
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Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
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thiamine diphosphate Note: Binds 1 thiamine pyrophosphate per subunit. |
Activity regulation |
The C-terminus inhibits activity; it has to move for the enzyme to be active. Activated by lipid-binding, which occurs via the C-terminus. |
Subunit |
Homotetramer. |
case not defined <Property:Membrane> or <Property:Membrane=1>
Subcellular location |
Cell membrane; Peripheral membrane protein; Cytoplasmic side. |
else case <Property:Membrane=2>
Subcellular location |
Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. |
end case
Domain |
Has 4 domains; the Pyr domain which binds the pyrimidine moiety of the thiamine pyrophosphate cofactor, the FAD-binding domain, the PP-binding domain which binds the pyrophosphate portion of thiamine pyrophosphate and the C-terminal membrane binding region. The C-terminus is held closely against the rest of the protein and covers the active site; during activation it unfolds from the rest of the protein and forms an amphipathic helix upon membrane binding, exposing the active site. |
Similarity |
Belongs to the TPP enzyme family. |
case defined <Property:Membrane> and <Property:Membrane=2>
end case
GO:0005886; Cellular component: plasma membrane.
GO:0050660; Molecular function: flavin adenine dinucleotide binding.
GO:0008289; Molecular function: lipid binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0052737; Molecular function: pyruvate dehydrogenase (quinone) activity.
GO:0030976; Molecular function: thiamine pyrophosphate binding.
GO:0042867; Biological process: pyruvate catabolic process.
GO:0048039; Molecular function: ubiquinone binding.
General |
Coiled_coil; -; 0-unlimited; trigger=yes; |
From: POXB_ECOLI (P07003) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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NP_BIND
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251
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254
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FAD
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T-G-L-[IL]
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NP_BIND
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274
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278
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FAD
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[TS]-x-[FL]-P-[YF]
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REGION
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1
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182
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Pyr domain
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REGION
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183
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334
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FAD-binding domain
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REGION
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335
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530
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PP-binding domain
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REGION
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406
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408
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Thiamine pyrophosphate binding
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[GA]-[ST]-M
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REGION
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433
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435
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Thiamine pyrophosphate binding
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D-G-G
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REGION
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460
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466
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Thiamine pyrophosphate binding
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REGION
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531
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572
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Membrane-binding domain
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METAL
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433
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433
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Magnesium
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D
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METAL
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460
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460
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Magnesium
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N
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BINDING
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50
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50
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Thiamine pyrophosphate
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E
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BINDING
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292
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292
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FAD
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D
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SITE
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465
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465
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Moves into active site upon enzyme activation, plays a role in electron transfer
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F
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Additional information
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Size range |
528-616 amino acids |
Related rules |
None |
Fusion |
None |