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HAMAP rule MF_00852

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General rule information [?]

Accession MF_00852
Dates 30-APR-2020 (Created)
17-FEB-2023 (Last updated, Version 7)
Name BioU
Bacteria; Cyanobacteriota
Archaea; Halobacteria
Template Q55650 (BIOU_SYNY3)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=(S)-8-amino-7-oxononanoate synthase BioU;
AltName: Full=8-amino-7-oxononanoate carboxylating dehydrogenase;
Gene name

Comments [?]

Function A 'suicide' enzyme that participates in biotin synthesis. Catalyzes the formation of (S)-8-amino-7-oxononanoate (DAN-carbamic acid) from (7R,8S)-8-amino-7-(carboxyamino)nonanoate (DAN), a function equivalent to the cannonical BioA reaction and the first half-reaction of BioD. The cellular requirement for biotin is thought be low enough that this single turnover enzyme supplies a sufficient amount of the cofactor. Overall it catalyzes three reactions: formation of a covalent linkage with 8-amino-7-oxononanoate to yield a BioU-DAN conjugate at the epsilon-amino group of Lys124 of BioU using NAD(P)H, carboxylation of the conjugate to form BioU-DAN-carbamic acid, and release of DAN-carbamic acid using NAD(P)+.
Catalytic activity RHEA:63660: (8S)-8-amino-7-oxononanoate + CO2 + L-lysyl-[protein] = (7R,8S)-8-amino-7-(carboxyamino)nonanoate + (S)-2-amino-6-oxohexanoyl-[protein] + 2 H(+)
PhysiologicalDirection=right-to-left (RHEA:63662)
RHEA:63664: (8S)-8-amino-7-oxononanoate + H(+) + L-lysyl-[protein] + NADPH = H2O + N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + NADP(+)
PhysiologicalDirection=right-to-left (RHEA:63666)
RHEA:63668: CO2 + H2O + N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + NADP(+) = (7R,8S)-8-amino-7-(carboxyamino)nonanoate + (S)-2-amino-6-oxohexanoyl-[protein] + 3 H(+) + NADPH
PhysiologicalDirection=right-to-left (RHEA:63670)
RHEA:63672: (8S)-8-amino-7-oxononanoate + H(+) + L-lysyl-[protein] + NADH = H2O + N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + NAD(+)
PhysiologicalDirection=right-to-left (RHEA:63674)
RHEA:63676: CO2 + H2O + N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + NAD(+) = (7R,8S)-8-amino-7-(carboxyamino)nonanoate + (S)-2-amino-6-oxohexanoyl-[protein] + 3 H(+) + NADH
PhysiologicalDirection=right-to-left (RHEA:63678)
Pathway Cofactor biosynthesis; biotin biosynthesis.
Subunit Monomer.
Miscellaneous In cannonical biotin synthesis a pimeloyl-conjugate is transformed into biotin by the subsequent action of BioF, BioA, BioD and BioB. This enzyme replaces BioA and performs the first half-reaction of BioD.
Similarity Belongs to the BioU family.

Keywords [?]

Gene Ontology [?]

GO:0009102; Biological process: biotin biosynthetic process.
GO:0050661; Molecular function: NADP binding.
GO:0051287; Molecular function: NAD binding.

Features [?]

From: BIOU_SYNY3 (Q55650)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     15     19       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     G-[FYT]-G-G-[LI]  
BINDING     190     191       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     [ASG]-T  
ACT_SITE     124     124       Nucleophile     K  
ACT_SITE     194     194       Proton acceptor     E  
ACT_SITE     198     198       Proton donor and proton acceptor     H  
BINDING (Optional)     60     60       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     Y  
MOD_RES     124     124       Allysine     K  

Additional information [?]

Size range 315-385 amino acids
Related rules None
Fusion None