HAMAP rule MF_00852
General rule information
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| Accession | MF_00852 |
| Dates | 30-APR-2020 (Created) 17-FEB-2023 (Last updated, Version 7) |
| Name | BioU |
| Scope | Bacteria; Cyanobacteriota
Archaea; Halobacteria |
| Template | Q55650 (BIOU_SYNY3) |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | A 'suicide' enzyme that participates in biotin synthesis. Catalyzes the formation of (S)-8-amino-7-oxononanoate (DAN-carbamic acid) from (7R,8S)-8-amino-7-(carboxyamino)nonanoate (DAN), a function equivalent to the cannonical BioA reaction and the first half-reaction of BioD. The cellular requirement for biotin is thought be low enough that this single turnover enzyme supplies a sufficient amount of the cofactor. Overall it catalyzes three reactions: formation of a covalent linkage with 8-amino-7-oxononanoate to yield a BioU-DAN conjugate at the epsilon-amino group of Lys124 of BioU using NAD(P)H, carboxylation of the conjugate to form BioU-DAN-carbamic acid, and release of DAN-carbamic acid using NAD(P)+. |
| Catalytic activity | RHEA:63660: (8S)-8-amino-7-oxononanoate + CO2 + L-lysyl-[protein] = (7R,8S)-8-amino-7-(carboxyamino)nonanoate + (S)-2-amino-6-oxohexanoyl-[protein] + 2 H(+)
EC 2.6.1.121 PhysiologicalDirection=right-to-left (RHEA:63662) |
| RHEA:63664: (8S)-8-amino-7-oxononanoate + H(+) + L-lysyl-[protein] + NADPH = H2O + N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + NADP(+)
PhysiologicalDirection=right-to-left (RHEA:63666) |
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| RHEA:63668: CO2 + H2O + N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + NADP(+) = (7R,8S)-8-amino-7-(carboxyamino)nonanoate + (S)-2-amino-6-oxohexanoyl-[protein] + 3 H(+) + NADPH
PhysiologicalDirection=right-to-left (RHEA:63670) |
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| RHEA:63672: (8S)-8-amino-7-oxononanoate + H(+) + L-lysyl-[protein] + NADH = H2O + N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + NAD(+)
PhysiologicalDirection=right-to-left (RHEA:63674) |
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| RHEA:63676: CO2 + H2O + N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + NAD(+) = (7R,8S)-8-amino-7-(carboxyamino)nonanoate + (S)-2-amino-6-oxohexanoyl-[protein] + 3 H(+) + NADH
PhysiologicalDirection=right-to-left (RHEA:63678) |
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| Pathway | Cofactor biosynthesis; biotin biosynthesis. |
| Subunit | Monomer. |
| Miscellaneous | In cannonical biotin synthesis a pimeloyl-conjugate is transformed into biotin by the subsequent action of BioF, BioA, BioD and BioB. This enzyme replaces BioA and performs the first half-reaction of BioD. |
| Similarity | Belongs to the BioU family. |
Keywords
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Gene Ontology
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GO:0009102; Biological process: biotin biosynthetic process.
GO:0050661; Molecular function: NADP binding.
GO:0051287; Molecular function: NAD binding.
GO:0050661; Molecular function: NADP binding.
GO:0051287; Molecular function: NAD binding.
Features
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| From: BIOU_SYNY3 (Q55650) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 15 | 19 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 | G-[FYT]-G-G-[LI] | ||||||||
| BINDING | 190 | 191 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 | [ASG]-T | ||||||||
| ACT_SITE | 124 | 124 | Nucleophile | K | ||||||||
| ACT_SITE | 194 | 194 | Proton acceptor | E | ||||||||
| ACT_SITE | 198 | 198 | Proton donor and proton acceptor | H | ||||||||
| BINDING (Optional) | 60 | 60 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 | Y | ||||||||
| MOD_RES | 124 | 124 | Allysine | K | ||||||||
Additional information
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| Size range | 315-385 amino acids |
| Related rules | None |
| Fusion | None |