Function |
A 'suicide' enzyme that participates in biotin synthesis. Catalyzes the formation of (S)-8-amino-7-oxononanoate (DAN-carbamic acid) from (7R,8S)-8-amino-7-(carboxyamino)nonanoate (DAN), a function equivalent to the cannonical BioA reaction and the first half-reaction of BioD. The cellular requirement for biotin is thought be low enough that this single turnover enzyme supplies a sufficient amount of the cofactor. Overall it catalyzes three reactions: formation of a covalent linkage with 8-amino-7-oxononanoate to yield a BioU-DAN conjugate at the epsilon-amino group of Lys124 of BioU using NAD(P)H, carboxylation of the conjugate to form BioU-DAN-carbamic acid, and release of DAN-carbamic acid using NAD(P)+. |
Catalytic activity |
RHEA:63660: (S)-8-amino-7-oxononanoate + CO2 + L-lysyl-[protein] = (7R,8S)-8-amino-7-(carboxyamino)nonanoate + (S)-2-amino-6-oxohexanoyl-[protein] + 2 H(+)
PhysiologicalDirection=right-to-left
(RHEA:63662)
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RHEA:63664: (S)-8-amino-7-oxononanoate + H(+) + L-lysyl-[protein] + NADPH = H2O + N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + NADP(+)
PhysiologicalDirection=right-to-left
(RHEA:63666)
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RHEA:63668: CO2 + H2O + N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + NADP(+) = (7R,8S)-8-amino-7-(carboxyamino)nonanoate + (S)-2-amino-6-oxohexanoyl-[protein] + 3 H(+) + NADPH
PhysiologicalDirection=right-to-left
(RHEA:63670)
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RHEA:63672: (S)-8-amino-7-oxononanoate + H(+) + L-lysyl-[protein] + NADH = H2O + N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + NAD(+)
PhysiologicalDirection=right-to-left
(RHEA:63674)
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RHEA:63676: CO2 + H2O + N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + NAD(+) = (7R,8S)-8-amino-7-(carboxyamino)nonanoate + (S)-2-amino-6-oxohexanoyl-[protein] + 3 H(+) + NADH
PhysiologicalDirection=right-to-left
(RHEA:63678)
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Pathway |
Cofactor biosynthesis; biotin biosynthesis. |
Subunit |
Monomer. |
Miscellaneous |
In cannonical biotin synthesis a pimeloyl-conjugate is transformed into biotin by the subsequent action of BioF, BioA, BioD and BioB. This enzyme replaces BioA and performs the first half-reaction of BioD. |
Similarity |
Belongs to the BioU family. |