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HAMAP rule MF_00863

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General rule information [?]

Accession MF_00863
Dates 18-JUN-2021 (Created)
1-JUN-2023 (Last updated, Version 7)
Name RNApol_arch_Rpo1N
Scope(s) Archaea
Template(s) B8YB53 (RPO1N_SACSH); P11512 (RPO1N_SULAC); Q980R2 (RPO1N_SACS2); [ Recover all ]
Triggered by HAMAP; MF_00863 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RPO1N
Protein name RecName: Full=DNA-directed RNA polymerase subunit Rpo1N;
                 EC=2.7.7.6;
AltName: Full=DNA-directed RNA polymerase subunit A';
Gene name Name=rpo1N; Synonyms=rpoA1;

Comments [?]

FUNCTIONDNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain.
CATALYTIC ACTIVITY Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.6;
case <FTGroup:3>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
end case
case <FTGroup:1> and <FTGroup:2>
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds at least 2 Zn(2+) per subunit.
end case
SUBUNITPart of the RNA polymerase complex.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the RNA polymerase beta' chain family.

Keywords [?]

Cytoplasm
DNA-binding
DNA-directed RNA polymerase
case <FTGroup:3>
Magnesium
end case
Metal-binding
Nucleotidyltransferase
Transcription
Transferase
case <FTGroup:1> and <FTGroup:2>
Zinc
end case

Gene Ontology [?]

GO:0005737; Cellular component:cytoplasm
GO:0003677; Molecular function:DNA binding
GO:0003899; Molecular function:DNA-directed 5'-3' RNA polymerase activity
case <FTGroup:3>
GO:0000287; Molecular function:magnesium ion binding
end case
GO:0006351; Biological process:DNA-templated transcription
case <FTGroup:1> and <FTGroup:2>
GO:0008270; Molecular function:zinc ion binding
end case

Cross-references [?]

Pfam PF00623; RNA_pol_Rpb1_2; 1-2;
Pfam PF04983; RNA_pol_Rpb1_3; 1;
Pfam PF04997; RNA_pol_Rpb1_1; 1;
Pfam PF04998; RNA_pol_Rpb1_5; 1;
Pfam PF05000; RNA_pol_Rpb1_4; 1;
NCBIfam TIGR02390; RNA_pol_rpoA1; 1;

Features [?]

From: RPO1N_SACSH (B8YB53)
Key From To Description Tag Condition FTGroup
BINDING 58 58 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"		 C 1
BINDING 61 61 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"		 C 1
BINDING 68 68 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"		 C 1
BINDING 71 71 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"		 H 1
BINDING 98 98 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"		 C 2
BINDING 101 101 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"		 C 2
BINDING 146 146 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"		 C 2
BINDING 149 149 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"		 C 2
BINDING 456 456 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 D 3
BINDING 458 458 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 D 3
BINDING 460 460 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 D 3

Additional information [?]

Size range 820-1070 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Intein deleted in METJA. In some archaea (including Thaumarchaeota and Korarchaeum cryptofilum) Rpo1N and Rpo1C are fused. A third Zn(2+) is present in S.acidocaldarius; the residues which bind this Zn are conserved in other known Sulfolobales and a few other archaea.



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