HAMAP rule MF_00863
General rule information
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Accession | MF_00863 |
Dates | 18-JUN-2021 (Created) 19-NOV-2022 (Last updated, Version 6) |
Name | RNApol_arch_Rpo1N |
Scope | Archaea |
Templates | B8YB53 (RPO1N_SACSH); P11512 (RPO1N_SULAC); Q980R2 (RPO1N_SACS2): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
|
Gene name |
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Comments
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Function | DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain. |
Catalytic activity | RHEA:21248: a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
EC 2.7.7.6 |
case <FTGroup:3>
Cofactor | Mg(2+) |
end case
case <FTGroup:1> and <FTGroup:2>
Cofactor | Zn(2+) Note: Binds at least 2 Zn(2+) per subunit |
end case
Subunit | Part of the RNA polymerase complex. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the RNA polymerase beta' chain family. |
Cross-references
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Pfam | PF00623; RNA_pol_Rpb1_2; 1-2; |
PF04983; RNA_pol_Rpb1_3; 1; | |
PF04997; RNA_pol_Rpb1_1; 1; | |
PF04998; RNA_pol_Rpb1_5; 1; | |
PF05000; RNA_pol_Rpb1_4; 1; | |
TIGRFAMs | TIGR02390; RNA_pol_rpoA1; 1; |
Keywords
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case <FTGroup:3>
end case
case <FTGroup:1> and <FTGroup:2>
end case
Gene Ontology
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GO:0005737; Cellular component: cytoplasm.
GO:0003677; Molecular function: DNA binding.
GO:0003899; Molecular function: DNA-directed 5'-3' RNA polymerase activity.
GO:0003677; Molecular function: DNA binding.
GO:0003899; Molecular function: DNA-directed 5'-3' RNA polymerase activity.
case <FTGroup:3>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0006351; Biological process: DNA-templated transcription.
case <FTGroup:1> and <FTGroup:2>
GO:0008270; Molecular function: zinc ion binding.
end case
Features
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From: RPO1N_SACSH (B8YB53) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 58 | 58 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 | C | 1 | |||||||
BINDING | 61 | 61 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 | C | 1 | |||||||
BINDING | 68 | 68 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 | C | 1 | |||||||
BINDING | 71 | 71 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 | H | 1 | |||||||
BINDING | 98 | 98 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 | C | 2 | |||||||
BINDING | 101 | 101 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 | C | 2 | |||||||
BINDING | 146 | 146 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 | C | 2 | |||||||
BINDING | 149 | 149 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 | C | 2 | |||||||
BINDING | 456 | 456 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | D | 3 | |||||||
BINDING | 458 | 458 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | D | 3 | |||||||
BINDING | 460 | 460 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | D | 3 |
Additional information
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Size range | 820-1070 amino acids |
Related rules | None |
Fusion | None |
Comments | Intein deleted in METJA. In some archaea (including Thaumarchaeota and Korarchaeum cryptofilum) Rpo1N and Rpo1C are fused. A third Zn(2+) is present in S.acidocaldarius; the residues which bind this Zn are conserved in other known Sulfolobales and a few other archaea. |