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Annotation rule MF_00863 |
General rule information
[?]
Accession |
MF_00863 |
Dates |
18-JUN-2021 (Created) 28-JUN-2022 (Last updated, Version 5) |
Propagated annotation
[?]
Identifier, protein and gene names
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Protein name |
RecName:
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Full=DNA-directed RNA polymerase subunit Rpo1N;
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EC 2.7.7.6;
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AltName:
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Full=DNA-directed RNA polymerase subunit A';
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Function |
DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain. |
Catalytic activity |
RHEA:21248: a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
EC 2.7.7.6
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case <FTGroup:3>
end case
case <FTGroup:1> and <FTGroup:2>
Cofactor |
Zn(2+) Note: Binds at least 2 Zn(2+) per subunit |
end case
Subunit |
Part of the RNA polymerase complex. |
Subcellular location |
Cytoplasm. |
Similarity |
Belongs to the RNA polymerase beta' chain family. |
case <FTGroup:3>
end case
case <FTGroup:1> and <FTGroup:2>
end case
GO:0005737; Cellular component: cytoplasm.
GO:0003677; Molecular function: DNA binding.
GO:0003899; Molecular function: DNA-directed 5'-3' RNA polymerase activity.
case <FTGroup:3>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0006351; Biological process: DNA-templated transcription.
case <FTGroup:1> and <FTGroup:2>
end case
From: RPO1N_SACSH (B8YB53) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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BINDING
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58
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58
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/ligand="Zn(2+)" /ligand_id="29105" /ligand_label="1
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C
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1
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BINDING
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61
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61
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/ligand="Zn(2+)" /ligand_id="29105" /ligand_label="1
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|
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C
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1
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BINDING
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68
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68
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/ligand="Zn(2+)" /ligand_id="29105" /ligand_label="1
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C
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1
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BINDING
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71
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71
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/ligand="Zn(2+)" /ligand_id="29105" /ligand_label="1
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H
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1
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BINDING
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98
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98
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/ligand="Zn(2+)" /ligand_id="29105" /ligand_label="2
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C
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2
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BINDING
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101
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101
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/ligand="Zn(2+)" /ligand_id="29105" /ligand_label="2
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C
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2
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BINDING
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146
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146
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/ligand="Zn(2+)" /ligand_id="29105" /ligand_label="2
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C
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2
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BINDING
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149
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149
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/ligand="Zn(2+)" /ligand_id="29105" /ligand_label="2
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C
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2
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BINDING
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456
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456
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/ligand="Mg(2+)" /ligand_id="18420
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D
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3
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BINDING
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458
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458
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/ligand="Mg(2+)" /ligand_id="18420
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D
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3
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BINDING
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460
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460
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/ligand="Mg(2+)" /ligand_id="18420
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D
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3
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Additional information
[?]
Size range |
820-1070 amino acids |
Related rules |
None |
Fusion |
None |
Comments |
Intein deleted in METJA. In some archaea (including Thaumarchaeota and Korarchaeum cryptofilum) Rpo1N and Rpo1C are fused. A third Zn(2+) is present in S.acidocaldarius; the residues which bind this Zn are conserved in other known Sulfolobales and a few other archaea. |