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Annotation rule MF_00917
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General rule information [?]

Accession MF_00917
Dates 8-FEB-2012 (Created)
19-NOV-2019 (Last updated, Version 8)
Name QueE
Templates O31677 (QUEE_BACSU); A0A0H3KB22 (QUEE_BURM1): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Bacteria>
Protein name
RecName: Full=7-carboxy-7-deazaguanine synthase;
Short=CDG synthase;
AltName: Full=Queuosine biosynthesis protein QueE;
else case <OC:Archaea>
Protein name
RecName: Full=7-carboxy-7-deazaguanine synthase;
Short=CDG synthase;
AltName: Full=Archaeosine biosynthesis protein QueE;
end case
Gene name

Comments [?]

Function Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.
Catalytic activity RHEA:27974: 6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7-deazaguanine + NH4(+)
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Note: Binds 1 S-adenosyl-L-methionine per subunit.
Pathway Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.
Subunit Homodimer.
Similarity Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.

Keywords [?]

case <OC:Bacteria>
end case

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0016840; Molecular function: carbon-nitrogen lyase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:1904047; Molecular function: S-adenosyl-L-methionine binding.
case <OC:Bacteria>
GO:0008616; Biological process: queuosine biosynthetic process.
end case

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
TIGRFAMs TIGR04508; queE_Cx14CxxC; 1;
TIGR03365; Bsubt_queE; 1;
TIGR04322; rSAM_QueE_Ecoli; 1;
TIGR03963; rSAM_QueE_Clost; 1;
PIRSF PIRSF000370; QueE; 1;

Features [?]

From: QUEE_BURM1 (A0A0H3KB22)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     12     14       Substrate binding     x-x-G  
REGION     48     50       S-adenosyl-L-methionine binding     [FYW]-x-D  
REGION (Optional)     133     135       S-adenosyl-L-methionine binding     S-x-K  
REGION (Optional)     173     176       S-adenosyl-L-methionine binding     Q-x-x-D  
METAL     31     31       Iron-sulfur (4Fe-4S-S-AdoMet)     C  
METAL     46     46       Iron-sulfur (4Fe-4S-S-AdoMet)     C  
METAL     49     49       Iron-sulfur (4Fe-4S-S-AdoMet)     C  
METAL (Optional)     51     51       Magnesium     [TS]  
BINDING     27     27       Substrate     R  
BINDING     90     90       Substrate     [TS]  
BINDING     92     92       S-adenosyl-L-methionine; via carbonyl oxygen     G  
BINDING (Optional)     Cter     Cter       Substrate; via carboxylate     P  

Additional information [?]

Size range 180-290 amino acids
Related rules None
Fusion None