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HAMAP rule MF_00917

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General rule information [?]

Accession MF_00917
Dates 8-FEB-2012 (Created)
1-JUN-2023 (Last updated, Version 12)
Name QueE
Scope(s) Bacteria
Template(s) O31677 (QUEE_BACSU); A0A0H3KB22 (QUEE_BURM1); [ Recover all ]
Triggered by HAMAP; MF_00917 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier QUEE
case <OC:Bacteria>
Protein name RecName: Full=7-carboxy-7-deazaguanine synthase;
                 Short=CDG synthase;
AltName: Full=Queuosine biosynthesis protein QueE;
else case <OC:Archaea>
Protein name RecName: Full=7-carboxy-7-deazaguanine synthase;
                 Short=CDG synthase;
AltName: Full=Archaeosine biosynthesis protein QueE;
end case
Gene name Name=queE;

Comments [?]

FUNCTIONCatalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7- deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.
CATALYTIC ACTIVITY Reaction=6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7- deazaguanine + NH4(+); Xref=Rhea:RHEA:27974, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:61032, ChEBI:CHEBI:61036; EC=;
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.;
COFACTOR Name=S-adenosyl-L-methionine; Xref=ChEBI:CHEBI:59789; Note=Binds 1 S-adenosyl-L-methionine per subunit.;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
PATHWAYPurine metabolism; 7-cyano-7-deazaguanine biosynthesis.
SIMILARITYBelongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family.

Keywords [?]

Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0016840; Molecular function:carbon-nitrogen lyase activity
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
GO:1904047; Molecular function:S-adenosyl-L-methionine binding
case <OCellular component:Bacteria>
GO:0008616; Biological process:queuosine biosynthetic process
end case

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
NCBIfam TIGR04508; queE_Cx14CxxC; 1;
NCBIfam TIGR03365; Bsubt_queE; 1;
NCBIfam TIGR04322; rSAM_QueE_Ecoli; 1;
NCBIfam TIGR03963; rSAM_QueE_Clost; 1;
PIRSF PIRSF000370; QueE; 1;

Features [?]

From: QUEE_BURM1 (A0A0H3KB22)
Key From To Description Tag Condition FTGroup
BINDING 12 14 /ligand="substrate" x-x-G
BINDING 48 50 /ligand="S-adenosyl-L-methionine"
BINDING 133 135 /ligand="S-adenosyl-L-methionine"
BINDING 173 176 /ligand="S-adenosyl-L-methionine"
BINDING 31 31 /ligand="[4Fe-4S] cluster"
BINDING 46 46 /ligand="[4Fe-4S] cluster"
BINDING 49 49 /ligand="[4Fe-4S] cluster"
BINDING 51 51 /ligand="Mg(2+)"
BINDING 27 27 /ligand="substrate" R
BINDING 90 90 /ligand="substrate" [TS]
BINDING 92 92 /ligand="S-adenosyl-L-methionine"
BINDING Cter Cter /ligand="substrate" P

Additional information [?]

Size range 180-290 amino acids
Related rules None
Fusion Nter: None Cter: None

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