HAMAP rule MF_00917
General rule information
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Accession | MF_00917 |
Dates | 8-FEB-2012 (Created)
1-JUN-2023 (Last updated, Version 12) |
Name | QueE |
Scope(s) |
Bacteria Archaea |
Template(s) | O31677 (QUEE_BACSU); A0A0H3KB22 (QUEE_BURM1); [ Recover all ] |
Triggered by |
HAMAP; MF_00917 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | QUEE |
case <OC:Bacteria> | |
Protein name | RecName: Full=7-carboxy-7-deazaguanine synthase; Short=CDG synthase; EC=4.3.99.3; AltName: Full=Queuosine biosynthesis protein QueE; |
else case <OC:Archaea> | |
Protein name | RecName: Full=7-carboxy-7-deazaguanine synthase; Short=CDG synthase; EC=4.3.99.3; AltName: Full=Archaeosine biosynthesis protein QueE; |
end case | |
Gene name | Name=queE; |
Comments
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FUNCTION | Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7- deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds. |
CATALYTIC ACTIVITY | Reaction=6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7- deazaguanine + NH4(+); Xref=Rhea:RHEA:27974, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:61032, ChEBI:CHEBI:61036; EC=4.3.99.3; |
COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; |
COFACTOR | Name=S-adenosyl-L-methionine; Xref=ChEBI:CHEBI:59789; Note=Binds 1 S-adenosyl-L-methionine per subunit.; |
COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
PATHWAY | Purine metabolism; 7-cyano-7-deazaguanine biosynthesis. |
SUBUNIT | Homodimer. |
SIMILARITY | Belongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family. |
Keywords
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4Fe-4S | |
Iron | |
Iron-sulfur | |
Lyase | |
Magnesium | |
Metal-binding | |
case <OC:Bacteria> | |
Queuosine biosynthesis | |
end case | |
S-adenosyl-L-methionine |
Gene Ontology
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GO:0000287; Molecular function:magnesium ion binding | |
GO:0016840; Molecular function:carbon-nitrogen lyase activity | |
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding | |
GO:1904047; Molecular function:S-adenosyl-L-methionine binding | |
case <OCellular component:Bacteria> | |
GO:0008616; Biological process:queuosine biosynthetic process | |
end case |
Cross-references
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Pfam | PF04055; Radical_SAM; 1; |
NCBIfam | TIGR04508; queE_Cx14CxxC; 1; |
NCBIfam | TIGR03365; Bsubt_queE; 1; |
NCBIfam | TIGR04322; rSAM_QueE_Ecoli; 1; |
NCBIfam | TIGR03963; rSAM_QueE_Clost; 1; |
PIRSF | PIRSF000370; QueE; 1; |
Features
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From: QUEE_BURM1 (A0A0H3KB22) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 12 | 14 | /ligand="substrate" | x-x-G | ||||||||
BINDING | 48 | 50 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[FYW]-x-D | ||||||||
BINDING | 133 | 135 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
S-x-K | ||||||||
BINDING | 173 | 176 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
Q-x-x-D | ||||||||
BINDING | 31 | 31 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
BINDING | 46 | 46 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
BINDING | 49 | 49 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
BINDING | 51 | 51 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[TS] | ||||||||
BINDING | 27 | 27 | /ligand="substrate" | R | ||||||||
BINDING | 90 | 90 | /ligand="substrate" | [TS] | ||||||||
BINDING | 92 | 92 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
G | ||||||||
BINDING | Cter | Cter | /ligand="substrate" | P |
Additional information
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Size range | 180-290 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |