HAMAP rule MF_00943

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_00943
Accession	MF_00943
Dates	13-FEB-2013 (Created) 14-MAY-2022 (Last updated, Version 7)
Name	Maleate_isomerase
Scope(s)	Bacteria
Template(s)	Q5YXQ1 (MAIA_NOCFA); O24766 (MAIA_ALCFA); Q9WX57 (MAIA_GEOSE); Q88FY4 (MAIA_PSEPK); [ Recover all ]
Triggered by	HAMAP; MF_00943 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	MAIA
Protein name	RecName: Full=Maleate isomerase; EC=5.2.1.1; AltName: Full=Maleate cis-trans isomerase;
Gene name	Name=maiA;

Comments [?]

FUNCTION	Catalyzes cis-trans isomerization of the C2-C3 double bond in maleate to yield fumarate.
CATALYTIC ACTIVITY	Reaction=maleate = fumarate; Xref=Rhea:RHEA:13169, ChEBI:CHEBI:29806, ChEBI:CHEBI:30780; EC=5.2.1.1;
SUBUNIT	Homodimer.
MISCELLANEOUS	Reaction is initiated by nucleophilic attack of cysteine at the double bond, yielding a covalent succinylcysteine-like intermediate.
SIMILARITY	Belongs to the maleate isomerase family.

Keywords [?]

Gene Ontology [?]

GO:0050076; Molecular function:maleate isomerase activity

Cross-references [?]

Pfam	PF17645; Amdase; 1;
PIRSF	PIRSF015736; MI; 1;

Features [?]

From: MAIA_NOCFA (Q5YXQ1)

Key

From

Description

Tag

Condition

FTGroup

BINDING

/ligand="substrate"

C-x-V

BINDING

195

196

/ligand="substrate"

V-Q

ACT_SITE

/note="Nucleophile"

ACT_SITE

194

/note="Proton donor"

BINDING

/ligand="substrate"

BINDING

133

/ligand="substrate"

BINDING

163

/ligand="substrate"

MOD_RES

/note="S-(2-succinyl)cysteine"

Additional information [?]