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| Annotation rule MF_00952 |
General rule information
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| Accession | MF_00952 |
| Dates | 22-MAR-2013 (Created) 19-NOV-2019 (Last updated, Version 9) |
| Name | Topoisom_1_prok |
| Scope | Bacteria
Archaea |
| Templates | P06612 (TOP1_ECOLI); P9WG49 (TOP1_MYCTU): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. |
| Catalytic activity | Reaction=ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.; EC=5.6.2.1; |
| Subunit | Monomer. |
| Similarity | Belongs to the type IA topoisomerase family. |
Keywords
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Gene Ontology
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GO:0003917; Molecular function: DNA topoisomerase type I (single strand cut, ATP-independent) activity.
GO:0006265; Biological process: DNA topological change.
GO:0006265; Biological process: DNA topological change.
Cross-references
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| PROSITE | PS00396; TOPOISOMERASE_I_PROK; 1; |
| PS50880; TOPRIM; 1; trigger=PRU00995; | |
| Pfam | PF08272; Topo_Zn_Ribbon; 0-2; |
| PF01131; Topoisom_bac; 1; | |
| PF01751; Toprim; 1; | |
| PF01396; zf-C4_Topoisom; 0-3; | |
| PRINTS | PR00417; PRTPISMRASEI; 1; |
| TIGRFAMs | TIGR01051; topA_bact; 0-1; |
| TIGR01057; topA_arch; 0-1; |
Features
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| From: TOP1_ECOLI (P06612) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION (Optional) | 192 | 197 | Interaction with DNA | S-x-G-R-V-Q | ||||||||
| ACT_SITE | 319 | 319 | O-(5'-phospho-DNA)-tyrosine intermediate | Y | ||||||||
| SITE (Optional) | 33 | 33 | Interaction with DNA | H | ||||||||
| SITE (Optional) | 168 | 168 | Interaction with DNA | R | ||||||||
| SITE (Optional) | 169 | 169 | Interaction with DNA | R | ||||||||
| SITE (Optional) | 172 | 172 | Interaction with DNA | D | ||||||||
| SITE (Optional) | 177 | 177 | Interaction with DNA | Y | ||||||||
| SITE (Optional) | 184 | 184 | Interaction with DNA | W | ||||||||
| SITE (Optional) | 321 | 321 | Interaction with DNA | R | ||||||||
| SITE (Optional) | 507 | 507 | Interaction with DNA | R | ||||||||
Additional information
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| Size range | 560-1100 amino acids |
| Related rules | None |
| Fusion | None |