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Annotation rule MF_00952 |
Accession | MF_00952 |
Dates | 22-MAR-2013 (Created) 19-NOV-2019 (Last updated, Version 9) |
Name | Topoisom_1_prok |
Scope | Bacteria
Archaea |
Templates | P06612 (TOP1_ECOLI); P9WG49 (TOP1_MYCTU): [Recover all] |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. |
Catalytic activity | Reaction=ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.; EC=5.6.2.1; |
Subunit | Monomer. |
Similarity | Belongs to the type IA topoisomerase family. |
PROSITE | PS00396; TOPOISOMERASE_I_PROK; 1; |
PS50880; TOPRIM; 1; trigger=PRU00995; | |
Pfam | PF08272; Topo_Zn_Ribbon; 0-2; |
PF01131; Topoisom_bac; 1; | |
PF01751; Toprim; 1; | |
PF01396; zf-C4_Topoisom; 0-3; | |
PRINTS | PR00417; PRTPISMRASEI; 1; |
TIGRFAMs | TIGR01051; topA_bact; 0-1; |
TIGR01057; topA_arch; 0-1; |
From: TOP1_ECOLI (P06612) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION (Optional) | 192 | 197 | Interaction with DNA | S-x-G-R-V-Q | ||||||||
ACT_SITE | 319 | 319 | O-(5'-phospho-DNA)-tyrosine intermediate | Y | ||||||||
SITE (Optional) | 33 | 33 | Interaction with DNA | H | ||||||||
SITE (Optional) | 168 | 168 | Interaction with DNA | R | ||||||||
SITE (Optional) | 169 | 169 | Interaction with DNA | R | ||||||||
SITE (Optional) | 172 | 172 | Interaction with DNA | D | ||||||||
SITE (Optional) | 177 | 177 | Interaction with DNA | Y | ||||||||
SITE (Optional) | 184 | 184 | Interaction with DNA | W | ||||||||
SITE (Optional) | 321 | 321 | Interaction with DNA | R | ||||||||
SITE (Optional) | 507 | 507 | Interaction with DNA | R |
Size range | 560-1100 amino acids |
Related rules | None |
Fusion | None |