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HAMAP rule MF_00970

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General rule information [?]

Accession MF_00970
Dates 7-AUG-2013 (Created)
1-JUN-2023 (Last updated, Version 12)
Name RNase_E
Scope(s) Bacteria
Template(s) P21513 (RNE_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00970 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RNE
Protein name RecName: Full=Ribonuclease E;
                 Short=RNase E;
Gene name Name=rne;

Comments [?]

FUNCTIONEndoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.
CATALYTIC ACTIVITY Reaction=Endonucleolytic cleavage of single-stranded RNA in A- and U- rich regions.; EC=;
case <FTGroup:2>
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 2 Zn(2+) ions per homotetramer.;
end case
case <FTGroup:1>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
end case
case <OC:Gammaproteobacteria>
SUBUNITComponent of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.
SUBUNITHomotetramer formed by a dimer of dimers.
end case
SUBCELLULAR LOCATIONCytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
SIMILARITYBelongs to the RNase E/G family. RNase E subfamily.

Keywords [?]

Gene Ontology [?]

GO:0004521; Molecular function:RNA endonuclease activity
case <FTGroup:1>
GO:0000287; Molecular function:magnesium ion binding
end case
GO:0003723; Molecular function:RNA binding
case <FTGroup:2>
GO:0008270; Molecular function:zinc ion binding
end case
GO:0006402; Biological process:mRNA catabolic process
GO:0006364; Biological process:rRNA processing
GO:0008033; Biological process:tRNA processing
GO:0005737; Cellular component:cytoplasm
GO:0009898; Cellular component:cytoplasmic side of plasma membrane

Cross-references [?]

PROSITE PS50126; S1; 1;
Pfam PF12111; PNPase_C; 0-1;
Pfam PF10150; RNase_E_G; 1;
Pfam PF00575; S1; 1;
NCBIfam TIGR00757; RNaseEG; 1;

Features [?]

From: RNE_ECOLI (P21513)
Key From To Description Tag Condition FTGroup
REGION 404 407 /note="Required for zinc-mediated homotetramerization and catalytic activity" C-x-x-C
BINDING 303 303 /ligand="Mg(2+)"
D 1
BINDING 346 346 /ligand="Mg(2+)"
D 1
BINDING 404 404 /ligand="Zn(2+)"
/ligand_note="ligand shared between dimeric partners"
C 2
BINDING 407 407 /ligand="Zn(2+)"
/ligand_note="ligand shared between dimeric partners"
C 2

Additional information [?]

Size range 560-1230 amino acids
Related rules None
Fusion Nter: None Cter: None

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