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Annotation rule MF_00970
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General rule information [?]

Accession MF_00970
Dates 7-AUG-2013 (Created)
21-APR-2020 (Last updated, Version 7)
Name RNase_E
Scope
Bacteria; Proteobacteria
Template P21513 (RNE_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
RNE
Protein name
RecName: Full=Ribonuclease E;
Short=RNase E;
EC 3.1.26.12;
Gene name
rne

Comments [?]

Function Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.
Catalytic activity Reaction=Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.; EC=3.1.26.12;
case <FTGroup:2>
Cofactor Zn(2+)
Note: Binds 2 Zn(2+) ions per homotetramer.
end case
case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
end case
case <OC:Gammaproteobacteria>
Subunit Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.
else
Subunit Homotetramer formed by a dimer of dimers.
end case
Subcellular location Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
Similarity Belongs to the RNase E/G family. RNase E subfamily.

Keywords [?]

Cell inner membrane
Cell membrane
Cytoplasm
Endonuclease
Hydrolase
case <FTGroup:1>
Magnesium
end case
Membrane
case <FTGroup:1> or <FTGroup:2>
Metal-binding
end case
Nuclease
RNA-binding
rRNA processing
tRNA processing
rRNA-binding
tRNA-binding
case <FTGroup:2>
Zinc
end case

Gene Ontology [?]

GO:0004521; Molecular function: endoribonuclease activity.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0003723; Molecular function: RNA binding.
case <FTGroup:2>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0006402; Biological process: mRNA catabolic process.
GO:0006364; Biological process: rRNA processing.
GO:0008033; Biological process: tRNA processing.
GO:0005737; Cellular component: cytoplasm.
GO:0009898; Cellular component: cytoplasmic side of plasma membrane.

Cross-references [?]

PROSITE PS50126; S1; 1; trigger=PRU00180;
Pfam PF12111; PNPase_C; 0-1;
PF10150; RNase_E_G; 1;
PF00575; S1; 1;
TIGRFAMs TIGR00757; RNaseEG; 1;

Features [?]

From: RNE_ECOLI (P21513)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     404     407       Required for zinc-mediated homotetramerization and catalytic activity     C-x-x-C  
METAL     303     303       Magnesium; catalytic     D   1
METAL     346     346       Magnesium; catalytic     D   1
METAL     404     404       Zinc; shared with dimeric partner     C   2
METAL     407     407       Zinc; shared with dimeric partner     C   2

Additional information [?]

Size range 560-1230 amino acids
Related rules None
Fusion None


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