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Annotation rule MF_00970 |
General rule information
[?]
Accession |
MF_00970 |
Dates |
7-AUG-2013 (Created) 21-APR-2020 (Last updated, Version 7) |
Scope |
Bacteria; Proteobacteria |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Function |
Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs. |
Catalytic activity |
Reaction=Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.; EC=3.1.26.12; |
case <FTGroup:2>
Cofactor |
Zn(2+) Note: Binds 2 Zn(2+) ions per homotetramer. |
end case
case <FTGroup:1>
Cofactor |
Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
end case
case <OC:Gammaproteobacteria>
Subunit |
Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers. |
else
Subunit |
Homotetramer formed by a dimer of dimers. |
end case
Subcellular location |
Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. |
Similarity |
Belongs to the RNase E/G family. RNase E subfamily. |
case <FTGroup:1>
end case
case <FTGroup:1> or <FTGroup:2>
end case
case <FTGroup:2>
end case
GO:0004521; Molecular function: endoribonuclease activity.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
case <FTGroup:2>
end case
GO:0006402; Biological process: mRNA catabolic process.
GO:0006364; Biological process: rRNA processing.
GO:0008033; Biological process: tRNA processing.
GO:0005737; Cellular component: cytoplasm.
GO:0009898; Cellular component: cytoplasmic side of plasma membrane.
From: RNE_ECOLI (P21513) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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REGION
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404
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407
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|
Required for zinc-mediated homotetramerization and catalytic activity
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C-x-x-C
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METAL
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303
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303
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Magnesium; catalytic
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D
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1
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METAL
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346
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346
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Magnesium; catalytic
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D
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1
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METAL
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404
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404
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Zinc; shared with dimeric partner
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C
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2
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METAL
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407
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407
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Zinc; shared with dimeric partner
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|
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C
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2
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Additional information
[?]
Size range |
560-1230 amino acids |
Related rules |
None |
Fusion |
None |