HAMAP rule MF_00970

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_00970
Accession	MF_00970
Dates	07-AUG-2013 (Created) 01-JUN-2023 (Last updated, Version 13)
Name	RNase_E
Scope(s)	Bacteria Pseudomonadota
Template(s)	P21513; [ Recover all ]
Triggered by	HAMAP; MF_00970 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	RNE
Protein name	RecName: Full=Ribonuclease E; Short=RNase E; EC=3.1.26.12;
Gene name	Name=rne;

Comments [?]

FUNCTION	Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.
CATALYTIC ACTIVITY	Reaction=Endonucleolytic cleavage of single-stranded RNA in A- and U- rich regions.; EC=3.1.26.12;
case <FTGroup:2>
COFACTOR	Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 2 Zn(2+) ions per homotetramer.;
end case
case <FTGroup:1>
COFACTOR	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
end case
case <OC:Gammaproteobacteria>
SUBUNIT	Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.
else
SUBUNIT	Homotetramer formed by a dimer of dimers.
end case
SUBCELLULAR LOCATION	Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
SIMILARITY	Belongs to the RNase E/G family. RNase E subfamily.

Keywords [?]

Gene Ontology [?]

GO:0004521; Molecular function:RNA endonuclease activity
case <FTGroup:1>
GO:0000287; Molecular function:magnesium ion binding
end case
GO:0003723; Molecular function:RNA binding
case <FTGroup:2>
GO:0008270; Molecular function:zinc ion binding
end case
GO:0006402; Biological process:mRNA catabolic process
GO:0006364; Biological process:rRNA processing
GO:0008033; Biological process:tRNA processing
GO:0005737; Cellular component:cytoplasm
GO:0009898; Cellular component:cytoplasmic side of plasma membrane

Cross-references [?]

Features [?]

From: RNE_ECOLI (P21513)

Key

From

Description

Tag

Condition

FTGroup

REGION

404

407

/note="Required for zinc-mediated homotetramerization and catalytic activity"

C-x-x-C

BINDING

303

/ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_note="catalytic"

BINDING

346

/ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_note="catalytic"

BINDING

404

/ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="ligand shared between dimeric partners"

BINDING

407

/ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="ligand shared between dimeric partners"

Additional information [?]