HAMAP rule MF_00974
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00974 |
| Accession | MF_00974 |
| Dates | 16-OCT-2013 (Created)
21-MAR-2025 (Last updated, Version 10) |
| Name | DNA_primase_DnaG |
| Scope(s) |
Bacteria |
| Template(s) | P0ABS5; O67465; Q9X4D0; [ Recover all ] |
| Triggered by |
HAMAP; MF_00974 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | DNAG |
| Protein name | RecName: Full=DNA primase; EC=2.7.7.101; |
| Gene name | Name=dnaG; |
Comments
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| FUNCTION | RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. |
| CATALYTIC ACTIVITY | Reaction=ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.; EC=2.7.7.101; |
| SUBUNIT | Monomer. Interacts with DnaB. |
| case <FTTag:zinc> | |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per monomer.; |
| DOMAIN | Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB- binding domain. |
| end case | |
| SIMILARITY | Belongs to the DnaG primase family. |
Keywords
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| DNA replication | |
| DNA-binding | |
| DNA-directed RNA polymerase | |
| Nucleotidyltransferase | |
| Primosome | |
| Transcription | |
| Transferase | |
| case <FTTag:zinc> | |
| Metal-binding | |
| Zinc | |
| Zinc-finger | |
| end case | |
Gene Ontology
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| GO:0003899; Molecular function:DNA-directed RNA polymerase activity | |
| case <FTTag:zinc> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
| GO:0006269; Biological process:DNA replication, synthesis of primer | |
Cross-references
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| PROSITE | PS50880; TOPRIM; 1; |
| Pfam | PF10410; DnaB_bind; 1; |
| Pfam | PF08278; DnaG_DnaB_bind; 1; |
| Pfam | PF13662; Toprim_4; 1; |
| Pfam | PF08275; Toprim_N; 1; |
| Pfam | PF01807; zf-CHC2; 1; |
| Pfam | PF01751; Toprim; 1; |
| NCBIfam | TIGR01391; dnaG; 1; |
| PIRSF | PIRSF002811; DnaG; 1; |
Features
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| From: DNAG_ECOLI (P0ABS5) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ZN_FING | 40 | 64 | /note="CHC2-type" | zinc | C-x(2)-H-x(17,18)-C-x(2,3)-C | |||||||
Additional information
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| Size range | 410-800 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |