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Annotation rule MF_00985 |
Accession | MF_00985 |
Dates | 17-JAN-2014 (Created) 19-NOV-2019 (Last updated, Version 5) |
Name | 2am3keto_CoA_ligase |
Scope | Bacteria |
Template | P0AB77 (KBL_ECOLI) |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Catalyzes the cleavage of 2-amino-3-ketobutyrate to glycine and acetyl-CoA. |
Catalytic activity | RHEA:20736: acetyl-CoA + glycine = (2S)-2-amino-3-oxobutanoate + CoA
EC 2.3.1.29 |
Cofactor | pyridoxal 5'-phosphate Note: Binds 1 pyridoxal phosphate per subunit. |
Pathway | Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 2/2. |
Subunit | Homodimer. |
Similarity | Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. |
PROSITE | PS00599; AA_TRANSFER_CLASS_2; 1; |
Pfam | PF00155; Aminotran_1_2; 1; |
TIGRFAMs | TIGR01822; 2am3keto_CoA; 1; |
From: KBL_ECOLI (P0AB77) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION (Optional) | 111 | 112 | Pyridoxal phosphate binding | C-[FY] | ||||||||
REGION (Optional) | 210 | 213 | Pyridoxal phosphate binding | [DE]-[DE]-S-H | ||||||||
REGION (Optional) | 241 | 244 | Pyridoxal phosphate binding | T-[LF]-G-K | ||||||||
REGION (Optional) | 274 | 275 | Pyridoxal phosphate binding; shared with dimeric partner | S-N | ||||||||
BINDING (Optional) | 136 | 136 | Substrate | H | ||||||||
BINDING (Optional) | 185 | 185 | Pyridoxal phosphate | S | ||||||||
BINDING (Optional) | 368 | 368 | Substrate | R | ||||||||
MOD_RES | 244 | 244 | N6-(pyridoxal phosphate)lysine | K |
Size range | 378-460 amino acids |
Related rules | None |
Fusion | None |