HAMAP rule MF_00985
General rule information
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Accession | MF_00985 |
Dates | 17-JAN-2014 (Created) 1-JUN-2023 (Last updated, Version 10) |
Name | 2am3keto_CoA_ligase |
Scope | Bacteria |
Template | P0AB77 (KBL_ECOLI) |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Catalyzes the cleavage of 2-amino-3-ketobutyrate to glycine and acetyl-CoA. |
Catalytic activity | RHEA:20736: acetyl-CoA + glycine = (2S)-2-amino-3-oxobutanoate + CoA
EC 2.3.1.29 |
Cofactor | pyridoxal 5'-phosphate Note: Binds 1 pyridoxal phosphate per subunit. |
Pathway | Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 2/2. |
Subunit | Homodimer. |
Similarity | Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. |
Keywords
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Gene Ontology
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GO:0008890; Molecular function: glycine C-acetyltransferase activity.
GO:0030170; Molecular function: pyridoxal phosphate binding.
GO:0019518; Biological process: L-threonine catabolic process to glycine.
GO:0030170; Molecular function: pyridoxal phosphate binding.
GO:0019518; Biological process: L-threonine catabolic process to glycine.
Cross-references
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PROSITE | PS00599; AA_TRANSFER_CLASS_2; 1; |
Pfam | PF00155; Aminotran_1_2; 1; |
NCBIfam | TIGR01822; 2am3keto_CoA; 1; |
Features
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From: KBL_ECOLI (P0AB77) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING (Optional) | 111 | 112 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners" /note="in other chain | C-[FY] | ||||||||
BINDING (Optional) | 210 | 213 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners" /note="in other chain | [DE]-[DE]-S-H | ||||||||
BINDING (Optional) | 241 | 244 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners" /note="in other chain | T-[LF]-G-K | ||||||||
BINDING (Optional) | 274 | 275 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners | S-N | ||||||||
BINDING (Optional) | 136 | 136 | /ligand="substrate | H | ||||||||
BINDING (Optional) | 185 | 185 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners" /note="in other chain | S | ||||||||
BINDING (Optional) | 368 | 368 | /ligand="substrate | R | ||||||||
MOD_RES | 244 | 244 | N6-(pyridoxal phosphate)lysine | K |
Additional information
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Size range | 378-460 amino acids |
Related rules | None |
Fusion | None |