HAMAP rule MF_00985
General rule information
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| Accession | MF_00985 |
| Dates | 17-JAN-2014 (Created)
1-JUN-2023 (Last updated, Version 10) |
| Name | 2am3keto_CoA_ligase |
| Scope(s) |
Bacteria |
| Template(s) | P0AB77 (KBL_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00985 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | KBL |
| Protein name | RecName: Full=2-amino-3-ketobutyrate coenzyme A ligase; Short=AKB ligase; EC=2.3.1.29; AltName: Full=Glycine acetyltransferase; |
| Gene name | Name=kbl; |
Comments
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| FUNCTION | Catalyzes the cleavage of 2-amino-3-ketobutyrate to glycine and acetyl-CoA. |
| CATALYTIC ACTIVITY | Reaction=acetyl-CoA + glycine = (2S)-2-amino-3-oxobutanoate + CoA; Xref=Rhea:RHEA:20736, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:57305, ChEBI:CHEBI:78948; EC=2.3.1.29; |
| COFACTOR | Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; Note=Binds 1 pyridoxal phosphate per subunit.; |
| PATHWAY | Amino-acid degradation; L-threonine degradation via oxydo- reductase pathway; glycine from L-threonine: step 2/2. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. |
Keywords
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Gene Ontology
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| GO:0008890; Molecular function:glycine C-acetyltransferase activity |
| GO:0030170; Molecular function:pyridoxal phosphate binding |
| GO:0019518; Biological process:L-threonine catabolic process to glycine |
Cross-references
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| PROSITE | PS00599; AA_TRANSFER_CLASS_2; 1; |
| Pfam | PF00155; Aminotran_1_2; 1; |
| NCBIfam | TIGR01822; 2am3keto_CoA; 1; |
Features
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| From: KBL_ECOLI (P0AB77) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 111 | 112 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
C-[FY] | ||||||||
| BINDING | 210 | 213 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
[DE]-[DE]-S-H | ||||||||
| BINDING | 241 | 244 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
T-[LF]-G-K | ||||||||
| BINDING | 274 | 275 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners" |
S-N | ||||||||
| BINDING | 136 | 136 | /ligand="substrate" | H | ||||||||
| BINDING | 185 | 185 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
S | ||||||||
| BINDING | 368 | 368 | /ligand="substrate" | R | ||||||||
| MOD_RES | 244 | 244 | /note="N6-(pyridoxal phosphate)lysine" | K | ||||||||
Additional information
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| Size range | 378-460 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |