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HAMAP rule MF_00985

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General rule information [?]

Accession MF_00985
Dates 17-JAN-2014 (Created)
1-JUN-2023 (Last updated, Version 10)
Name 2am3keto_CoA_ligase
Scope(s) Bacteria
Template(s) P0AB77 (KBL_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00985 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier KBL
Protein name RecName: Full=2-amino-3-ketobutyrate coenzyme A ligase;
                 Short=AKB ligase;
AltName: Full=Glycine acetyltransferase;
Gene name Name=kbl;

Comments [?]

FUNCTIONCatalyzes the cleavage of 2-amino-3-ketobutyrate to glycine and acetyl-CoA.
CATALYTIC ACTIVITY Reaction=acetyl-CoA + glycine = (2S)-2-amino-3-oxobutanoate + CoA; Xref=Rhea:RHEA:20736, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:57305, ChEBI:CHEBI:78948; EC=;
COFACTOR Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; Note=Binds 1 pyridoxal phosphate per subunit.;
PATHWAYAmino-acid degradation; L-threonine degradation via oxydo- reductase pathway; glycine from L-threonine: step 2/2.
SIMILARITYBelongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Keywords [?]

Gene Ontology [?]

GO:0008890; Molecular function:glycine C-acetyltransferase activity
GO:0030170; Molecular function:pyridoxal phosphate binding
GO:0019518; Biological process:L-threonine catabolic process to glycine

Cross-references [?]

Pfam PF00155; Aminotran_1_2; 1;
NCBIfam TIGR01822; 2am3keto_CoA; 1;

Features [?]

From: KBL_ECOLI (P0AB77)
Key From To Description Tag Condition FTGroup
BINDING 111 112 /ligand="pyridoxal 5'-phosphate"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
BINDING 210 213 /ligand="pyridoxal 5'-phosphate"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
BINDING 241 244 /ligand="pyridoxal 5'-phosphate"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
BINDING 274 275 /ligand="pyridoxal 5'-phosphate"
/ligand_note="ligand shared between dimeric partners"
BINDING 136 136 /ligand="substrate" H
BINDING 185 185 /ligand="pyridoxal 5'-phosphate"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
BINDING 368 368 /ligand="substrate" R
MOD_RES 244 244 /note="N6-(pyridoxal phosphate)lysine" K

Additional information [?]

Size range 378-460 amino acids
Related rules None
Fusion Nter: None Cter: None

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