HAMAP rule MF_00993

General rule information [?]

Accession	MF_00993
Dates	14-FEB-2014 (Created) 19-NOV-2022 (Last updated, Version 12)

Name

MqnE

Scope

Bacteria

Template

Q5SK48 (MQNE_THET8)

Triggered by

HAMAP; MF_00993 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

MQNE

Protein name

RecName:		Full=Aminodeoxyfutalosine synthase;
		Short=AFL synthase;
		Short=Aminofutalosine synthase;
		EC 2.5.1.120;
AltName:		Full=Menaquinone biosynthetic enzyme MqnE;

Gene name

mqnE

Comments [?]

Function	Radical SAM enzyme that catalyzes the addition of the adenosyl radical to the double bond of 3-[(1-carboxyvinyl)oxy]benzoate, leading to aminodeoxyfutalosine (AFL), a key intermediate in the formation of menaquinone (MK, vitamin K2) from chorismate.
Catalytic activity	RHEA:33075: 3-[(1-carboxyvinyl)-oxy]benzoate + H2O + S-adenosyl-L-methionine = 6-amino-6-deoxyfutalosine + H(+) + hydrogencarbonate + L-methionine EC 2.5.1.120

case <FTGroup:1>

Cofactor

[4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

end case

Pathway	Quinol/quinone metabolism; menaquinone biosynthesis.
Similarity	Belongs to the radical SAM superfamily. MqnE family.

Keywords [?]

case <FTGroup:1>

4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine

end case

Menaquinone biosynthesis
Transferase

Gene Ontology [?]

case <FTGroup:1>

GO:0005506; Molecular function: iron ion binding.

end case

GO:0016765; Molecular function: transferase activity, transferring alkyl or aryl (other than methyl) groups.
GO:0009234; Biological process: menaquinone biosynthetic process.

Cross-references [?]

Pfam	PF04055; Radical_SAM; 1;
TIGRFAMs	TIGR03700; mena_SCO4494; 1;
	TIGR00423; TIGR00423; 1;
PIRSF	PIRSF004762; CHP00423; 1;
PROSITE	PS51918; RADICAL_SAM; 1; trigger=PRU01266;

Features [?]

From: MQNE_THET8 (Q5SK48)

Key From To Description Tag Condition FTGroup

BINDING 69 69 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet C 1

BINDING 73 73 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet C 1

BINDING 76 76 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet C 1

Additional information [?]

Size range	335-420 amino acids
Related rules	None
Fusion	None

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