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HAMAP rule MF_00993

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General rule information [?]

Accession MF_00993
Dates 14-FEB-2014 (Created)
19-NOV-2022 (Last updated, Version 12)
Name MqnE
Template Q5SK48 (MQNE_THET8)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Aminodeoxyfutalosine synthase;
Short=AFL synthase;
Short=Aminofutalosine synthase;
AltName: Full=Menaquinone biosynthetic enzyme MqnE;
Gene name

Comments [?]

Function Radical SAM enzyme that catalyzes the addition of the adenosyl radical to the double bond of 3-[(1-carboxyvinyl)oxy]benzoate, leading to aminodeoxyfutalosine (AFL), a key intermediate in the formation of menaquinone (MK, vitamin K2) from chorismate.
Catalytic activity RHEA:33075: 3-[(1-carboxyvinyl)-oxy]benzoate + H2O + S-adenosyl-L-methionine = 6-amino-6-deoxyfutalosine + H(+) + hydrogencarbonate + L-methionine
case <FTGroup:1>
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
end case
Pathway Quinol/quinone metabolism; menaquinone biosynthesis.
Similarity Belongs to the radical SAM superfamily. MqnE family.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0005506; Molecular function: iron ion binding.
end case
GO:0016765; Molecular function: transferase activity, transferring alkyl or aryl (other than methyl) groups.
GO:0009234; Biological process: menaquinone biosynthetic process.

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
TIGRFAMs TIGR03700; mena_SCO4494; 1;
TIGR00423; TIGR00423; 1;
PIRSF PIRSF004762; CHP00423; 1;
PROSITE PS51918; RADICAL_SAM; 1; trigger=PRU01266;

Features [?]

From: MQNE_THET8 (Q5SK48)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     69     69       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet     C   1
BINDING     73     73       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet     C   1
BINDING     76     76       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet     C   1

Additional information [?]

Size range 335-420 amino acids
Related rules None
Fusion None