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HAMAP rule MF_00993

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General rule information [?]

Accession MF_00993
Dates 14-FEB-2014 (Created)
1-JUN-2023 (Last updated, Version 13)
Name MqnE
Scope(s) Bacteria
Template(s) Q5SK48 (MQNE_THET8); [ Recover all ]
Triggered by HAMAP; MF_00993 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MQNE
Protein name RecName: Full=Aminodeoxyfutalosine synthase;
                 Short=AFL synthase;
                 Short=Aminofutalosine synthase;
AltName: Full=Menaquinone biosynthetic enzyme MqnE;
Gene name Name=mqnE;

Comments [?]

FUNCTIONRadical SAM enzyme that catalyzes the addition of the adenosyl radical to the double bond of 3-[(1-carboxyvinyl)oxy]benzoate, leading to aminodeoxyfutalosine (AFL), a key intermediate in the formation of menaquinone (MK, vitamin K2) from chorismate.
CATALYTIC ACTIVITY Reaction=3-[(1-carboxyvinyl)-oxy]benzoate + H2O + S-adenosyl-L- methionine = 6-amino-6-deoxyfutalosine + H(+) + hydrogencarbonate + L-methionine; Xref=Rhea:RHEA:33075, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:64286, ChEBI:CHEBI:76981; EC=;
case <FTGroup:1>
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.;
end case
PATHWAYQuinol/quinone metabolism; menaquinone biosynthesis.
SIMILARITYBelongs to the radical SAM superfamily. MqnE family.

Keywords [?]

Gene Ontology [?]

case <FTGroup:1>
GO:0005506; Molecular function:iron ion binding
end case
GO:0016765; Molecular function:transferase activity, transferring alkyl or aryl (other than methyl) groups
GO:0009234; Biological process:menaquinone biosynthetic process

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
NCBIfam TIGR03700; mena_SCO4494; 1;
NCBIfam TIGR00423; TIGR00423; 1;
PIRSF PIRSF004762; CHP00423; 1;

Features [?]

From: MQNE_THET8 (Q5SK48)
Key From To Description Tag Condition FTGroup
BINDING 69 69 /ligand="[4Fe-4S] cluster"
C 1
BINDING 73 73 /ligand="[4Fe-4S] cluster"
C 1
BINDING 76 76 /ligand="[4Fe-4S] cluster"
C 1

Additional information [?]

Size range 335-420 amino acids
Related rules None
Fusion Nter: None Cter: None

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