HAMAP rule MF_01032
General rule information
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Accession | MF_01032 |
Dates | 10-SEP-2002 (Created) 1-JUN-2023 (Last updated, Version 32) |
Name | LeuD_type2 |
Scope | Bacteria
Archaea |
Templates | P04787 (LEUD1_SALTY); Q58667 (HACB_METJA): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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case <FTTag:HACNmotif>
Identifier |
|
Protein name |
|
Gene name |
|
else
Identifier |
|
Protein name |
|
Gene name |
|
end case
Comments
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case <FTTag:HACNmotif>
Function | Hydro-lyase with broad substrate specificity for cis-unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4-tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4-tricarboxylate), and its hydration to homoisocitrate ((1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate). Is also able to hydrate the analogous longer chain substrates cis-homo(2)-aconitate, cis-homo(3)-aconitate. All these reactions are part of the biosynthesis pathway of coenzyme B. |
Catalytic activity | RHEA:32303: (2R)-homocitrate = (2R,3S)-homoisocitrate
EC 4.2.1.114 |
Pathway | Organic acid metabolism; 2-oxosuberate biosynthesis. |
Subunit | Heterotetramer of 2 HacA and 2 HacB proteins. |
else
Function | Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate. |
Catalytic activity | RHEA:32287: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate
EC 4.2.1.33 |
Pathway | Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. |
Subunit | Heterodimer of LeuC and LeuD. |
end case
Similarity | Belongs to the LeuD family. LeuD type 2 subfamily. |
Keywords
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case not <FTTag:HACNmotif>
end case
Gene Ontology
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case <FTTag:HACNmotif>
GO:0004409; Molecular function: homoaconitate hydratase activity.
GO:0019298; Biological process: coenzyme B biosynthetic process.
GO:0019298; Biological process: coenzyme B biosynthetic process.
else
GO:0003861; Molecular function: 3-isopropylmalate dehydratase activity.
GO:0009098; Biological process: leucine biosynthetic process.
GO:0009098; Biological process: leucine biosynthetic process.
end case
Cross-references
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Features
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case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri>
From: HACB_METJA (Q58667) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
MOTIF (Optional) | 24 | 27 | YLRT | HACNmotif | Y-L-R-T | |||||||
SITE (Optional) | 26 | 26 | Critical for substrate specificity | R |
end case
Additional information
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Size range | 161-208 amino acids |
Related rules | MF_01031 (LEUD) |
Fusion | None |
Comments | The family member in THET2 is shown to function as a homoaconitase (for lysine biosynthesis), as well as one of the 2 copies found in METJA that catalyzes both the dehydration of (R)-homocitrate and the hydration of cis-homoaconitate for coenzyme B biosynthesis. The other copy in METJA functions both in leucine and isoleucine biosynthesis, since it catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate and between 2-methylmalate and 3-methylmalate. In Archaea, a motif that indicates the protein specificity has been determined (see PubMed=20170198): the presence of the YLRT motif likely indicates HACN activity, while proteins with the YLV(Y/I/M) sequence are IPMIs enzymes. |