HAMAP rule MF_01033
General rule information
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| Accession | MF_01033 |
| Dates | 1-OCT-2002 (Created)
1-JUN-2023 (Last updated, Version 41) |
| Name | LeuB_type1 |
| Scope(s) |
Bacteria |
| Template(s) | P30125 (LEU3_ECOLI); P37412 (LEU3_SALTY); P61495 (LEU3_THETH); Q56268 (LEU3_ACIFR); Q5SIY4 (LEU3_THET8); [ Recover all ] |
| Triggered by |
HAMAP; MF_01033 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | LEU3 |
| Protein name | RecName: Full=3-isopropylmalate dehydrogenase; EC=1.1.1.85; AltName: Full=3-IPM-DH; AltName: Full=Beta-IPM dehydrogenase; Short=IMDH; |
| Gene name | Name=leuB; |
Comments
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| FUNCTION | Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. |
| CATALYTIC ACTIVITY | Reaction=(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH; Xref=Rhea:RHEA:32271, ChEBI:CHEBI:16526, ChEBI:CHEBI:17865, ChEBI:CHEBI:35121, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.85; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit.; |
| PATHWAY | Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. |
| SUBUNIT | Homodimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily. |
Keywords
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| Cytoplasm |
| Amino-acid biosynthesis |
| Branched-chain amino acid biosynthesis |
| Leucine biosynthesis |
| Magnesium |
| Manganese |
| Metal-binding |
| NAD |
| Oxidoreductase |
Gene Ontology
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| GO:0003862; Molecular function:3-isopropylmalate dehydrogenase activity |
| GO:0009098; Biological process:leucine biosynthetic process |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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| From: LEU3_THET8 (Q5SIY4) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 74 | 87 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
G-[PYSAEKRT]-[KQHTAER]-x(0,3)-[WYV]-x(6,8)-P-E | ||||||||
| BINDING | 274 | 286 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
G-S-A-P-[DNT]-[IL]-x(4,5)-[AV]-N | ||||||||
| BINDING | 217 | 217 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 241 | 241 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 245 | 245 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 94 | 94 | /ligand="substrate" | R | ||||||||
| BINDING | 104 | 104 | /ligand="substrate" | R | ||||||||
| BINDING | 132 | 132 | /ligand="substrate" | R | ||||||||
| BINDING | 217 | 217 | /ligand="substrate" | D | ||||||||
| SITE | 139 | 139 | /note="Important for catalysis" | Y | ||||||||
| SITE | 185 | 185 | /note="Important for catalysis" | K | ||||||||
Additional information
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| Size range | 344-381 amino acids |
| Related rules |
MF_01035 |
| Fusion | Nter: None Cter: None |
| Comments | See MF_01035 for Actinomycetales leuB. Archaeal leuB are divergent and are not included in the alignment. |