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HAMAP rule MF_01033

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General rule information [?]

Accession MF_01033
Dates 1-OCT-2002 (Created)
1-JUN-2023 (Last updated, Version 41)
Name LeuB_type1
Scope(s) Bacteria
Template(s) P30125 (LEU3_ECOLI); P37412 (LEU3_SALTY); P61495 (LEU3_THETH); Q56268 (LEU3_ACIFR); Q5SIY4 (LEU3_THET8); [ Recover all ]
Triggered by HAMAP; MF_01033 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier LEU3
Protein name RecName: Full=3-isopropylmalate dehydrogenase;
AltName: Full=3-IPM-DH;
AltName: Full=Beta-IPM dehydrogenase;
Gene name Name=leuB;

Comments [?]

FUNCTIONCatalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
CATALYTIC ACTIVITY Reaction=(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH; Xref=Rhea:RHEA:32271, ChEBI:CHEBI:16526, ChEBI:CHEBI:17865, ChEBI:CHEBI:35121, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit.;
PATHWAYAmino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
SIMILARITYBelongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003862; Molecular function:3-isopropylmalate dehydrogenase activity
GO:0009098; Biological process:leucine biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00180; Iso_dh; 1;
NCBIfam TIGR00169; LeuB; 1;

Features [?]

From: LEU3_THET8 (Q5SIY4)
Key From To Description Tag Condition FTGroup
BINDING 74 87 /ligand="NAD(+)"
BINDING 274 286 /ligand="NAD(+)"
BINDING 217 217 /ligand="Mg(2+)"
D 1
BINDING 241 241 /ligand="Mg(2+)"
D 1
BINDING 245 245 /ligand="Mg(2+)"
D 1
BINDING 94 94 /ligand="substrate" R
BINDING 104 104 /ligand="substrate" R
BINDING 132 132 /ligand="substrate" R
BINDING 217 217 /ligand="substrate" D
SITE 139 139 /note="Important for catalysis" Y
SITE 185 185 /note="Important for catalysis" K

Additional information [?]

Size range 344-381 amino acids
Related rules MF_01035
Fusion Nter: None Cter: None
Comments See MF_01035 for Actinomycetales leuB. Archaeal leuB are divergent and are not included in the alignment.

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