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Annotation rule MF_01033
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General rule information [?]

Accession MF_01033
Dates 1-OCT-2002 (Created)
19-NOV-2019 (Last updated, Version 37)
Name LeuB_type1
Scope
Bacteria
Templates P30125 (LEU3_ECOLI); P37412 (LEU3_SALTY); P61495 (LEU3_THETH); Q56268 (LEU3_ACIFR); Q5SIY4 (LEU3_THET8): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
LEU3
Protein name
RecName: Full=3-isopropylmalate dehydrogenase;
EC 1.1.1.85;
AltName: Full=3-IPM-DH;
AltName: Full=Beta-IPM dehydrogenase;
Short=IMDH;
Gene name
leuB

Comments [?]

Function Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity RHEA:32271: (2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH
EC 1.1.1.85
Cofactor Mg(2+)
Mn(2+)
Note: Binds 1 Mg(2+) or Mn(2+) ion per subunit.
Pathway Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Keywords [?]

Cytoplasm
Amino-acid biosynthesis
Branched-chain amino acid biosynthesis
Leucine biosynthesis
Magnesium
Manganese
Metal-binding
NAD
Oxidoreductase

Gene Ontology [?]

GO:0003862; Molecular function: 3-isopropylmalate dehydrogenase activity.
GO:0009098; Biological process: leucine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00180; Iso_dh; 1;
TIGRFAMs TIGR00169; LeuB; 1;
PROSITE PS00470; IDH_IMDH; 1;

Features [?]

From: LEU3_THET8 (Q5SIY4)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND (Optional)     74     87       NAD     G-[PYSAEKRT]-[KQHTAER]-x(0,3)-[WYV]-x(6,8)-P-E  
NP_BIND (Optional)     274     286       NAD     G-S-A-P-[DNT]-[IL]-x(4,5)-[AV]-N  
METAL     217     217       Magnesium or manganese     D   1
METAL     241     241       Magnesium or manganese     D   1
METAL     245     245       Magnesium or manganese     D   1
BINDING     94     94       Substrate     R  
BINDING     104     104       Substrate     R  
BINDING     132     132       Substrate     R  
BINDING     217     217       Substrate     D  
SITE     139     139       Important for catalysis     Y  
SITE     185     185       Important for catalysis     K  

Additional information [?]

Size range 344-381 amino acids
Related rules MF_01035 (LEU3)
Fusion None
Comments See MF_01035 for Actinomycetales leuB. Archaeal leuB are divergent and are not included in the alignment.


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