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Annotation rule MF_01035 |
Accession | MF_01035 |
Dates | 1-OCT-2002 (Created) 19-NOV-2019 (Last updated, Version 31) |
Name | LeuB_type2 |
Scope | Bacteria; Actinobacteria |
Templates | P30125 (LEU3_ECOLI); P37412 (LEU3_SALTY); P61495 (LEU3_THETH); Q56268 (LEU3_ACIFR); Q5SIY4 (LEU3_THET8): [Recover all] |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. |
Catalytic activity | RHEA:32271: (2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH
EC 1.1.1.85 |
Cofactor | Mg(2+) Mn(2+) Note: Binds 1 Mg(2+) or Mn(2+) ion per subunit. |
Pathway | Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. |
Subunit | Homodimer. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily. |
From: LEU3_MYCTU (P9WKK9) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
NP_BIND | 271 | 283 | NAD | G-S-A-P-D-I-x(5)-A-[DN] | ||||||||
METAL | 211 | 211 | Magnesium or manganese | D | 1 | |||||||
METAL | 235 | 235 | Magnesium or manganese | D | 1 | |||||||
METAL | 239 | 239 | Magnesium or manganese | D | 1 | |||||||
BINDING | 87 | 87 | Substrate | R | ||||||||
BINDING | 97 | 97 | Substrate | R | ||||||||
BINDING | 121 | 121 | Substrate | R | ||||||||
BINDING | 211 | 211 | Substrate | D | ||||||||
SITE | 128 | 128 | Important for catalysis | Y | ||||||||
SITE | 178 | 178 | Important for catalysis | K |
Size range | 335-357 amino acids |
Related rules | MF_01033 (LEU3) |
Fusion | None |
Comments | See MF_01033 for other leuB |