HAMAP rule MF_01046

General rule information [?]

Accession	MF_01046
Dates	13-FEB-2003 (Created) 19-NOV-2022 (Last updated, Version 31)

Name	Deglycase_HchA

Scope

Bacteria; Staphylococcus

Bacteria; Gammaproteobacteria

Template

P31658 (HCHA_ECOLI)

Triggered by

HAMAP; MF_01046 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name

RecName:		Full=Protein/nucleic acid deglycase HchA;
		EC 3.1.2.-;
		EC 3.5.1.-;
		EC 3.5.1.124;
AltName:		Full=Maillard deglycase;

Gene name

hchA

Comments [?]

Function	Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of Schiff bases and advanced glycation endproducts (AGE). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Plays an important role in protecting cells from carbonyl stress.
Catalytic activity	RHEA:49548: H2O + N(omega)-(1-hydroxy-2-oxopropyl)-L-arginyl-[protein] = H(+) + L-arginyl-[protein] + lactate EC 3.5.1.124
	RHEA:49552: H2O + N(6)-(1-hydroxy-2-oxopropyl)-L-lysyl-[protein] = H(+) + L-lysyl-[protein] + lactate EC 3.5.1.124
	RHEA:49556: H2O + S-(1-hydroxy-2-oxopropyl)-L-cysteinyl-[protein] = H(+) + L-cysteinyl-[protein] + lactate EC 3.5.1.124
	RHEA:57188: H2O + N(omega)-(1-hydroxy-2-oxoethyl)-L-arginyl-[protein] = glycolate + H(+) + L-arginyl-[protein]
	RHEA:57192: H2O + N(6)-(1-hydroxy-2-oxoethyl)-L-lysyl-[protein] = glycolate + H(+) + L-lysyl-[protein]
	RHEA:57196: H2O + S-(1-hydroxy-2-oxoethyl)-L-cysteinyl-[protein] = glycolate + H(+) + L-cysteinyl-[protein]
	RHEA:57244: H2O + N(2)-(1-hydroxy-2-oxopropyl)-dGTP = dGTP + H(+) + lactate
	RHEA:57256: H2O + N(2)-(1-hydroxy-2-oxopropyl)-GTP = GTP + H(+) + lactate
	RHEA:57260: H2O + N(2)-(1-hydroxy-2-oxopropyl)-GDP = GDP + H(+) + lactate
	RHEA:57268: H2O + N(2)-(1-hydroxy-2-oxopropyl)-GMP = GMP + H(+) + lactate
	RHEA:57248: H2O + N(2)-(1-hydroxy-2-oxoethyl)-dGTP = dGTP + glycolate + H(+)
	RHEA:57252: H2O + N(2)-(1-hydroxy-2-oxoethyl)-GTP = glycolate + GTP + H(+)
	RHEA:57264: H2O + N(2)-(1-hydroxy-2-oxoethyl)-GDP = GDP + glycolate + H(+)
	RHEA:57304: H2O + N(2)-(1-hydroxy-2-oxoethyl)-GMP = glycolate + GMP + H(+)
	RHEA:57288: an N(2)-(1-hydroxy-2-oxopropyl)-guanosine in RNA + H2O = a guanosine in RNA + H(+) + lactate
	RHEA:57300: an N(2)-(1-hydroxy-2-oxopropyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + H(+) + lactate
	RHEA:57292: an N(2)-(1-hydroxy-2-oxoethyl)-guanosine in RNA + H2O = a guanosine in RNA + glycolate + H(+)
	RHEA:57296: an N(2)-(1-hydroxy-2-oxoethyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + glycolate + H(+)

case <OC:Enterobacterales>

Subunit

Homodimer.

end case

Subcellular location

Cytoplasm.

case <OC:Escherichia> or <OC:Shigella>

Induction

By heat shock.

end case

Similarity

Belongs to the peptidase C56 family. HchA subfamily.

Keywords [?]

Cytoplasm
DNA damage
DNA repair
Hydrolase
Stress response

case <FTGroup:1>

Metal-binding
Zinc

end case

Gene Ontology [?]

GO:0016790; Molecular function: thiolester hydrolase activity.
GO:0016811; Molecular function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides.
GO:0036524; Molecular function: protein deglycase activity.

case <FTGroup:1>

GO:0008270; Molecular function: zinc ion binding.

end case

GO:0006281; Biological process: DNA repair.
GO:0030091; Biological process: protein repair.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF01965; DJ-1_PfpI; 1;
PIRSF	PIRSF037798; Chaperone_HchA; 1;

Features [?]

From: HCHA_ECOLI (P31658)

Key From To Description Tag Condition FTGroup

ACT_SITE 185 185 Nucleophile C

BINDING (Optional) 86 86 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 [H] 1

BINDING (Optional) 91 91 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 [E] 1

BINDING (Optional) 123 123 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 [H] 1

Additional information [?]

Size range	282-292 amino acids
Related rules	None
Fusion	None

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