HAMAP rule MF_01046
General rule information
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Accession | MF_01046 |
Dates | 13-FEB-2003 (Created) 19-NOV-2022 (Last updated, Version 31) |
Name | Deglycase_HchA |
Scope | Bacteria; Staphylococcus
Bacteria; Gammaproteobacteria |
Template | P31658 (HCHA_ECOLI) |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Protein name |
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Gene name |
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Comments
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Function | Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of Schiff bases and advanced glycation endproducts (AGE). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Plays an important role in protecting cells from carbonyl stress. |
Catalytic activity | RHEA:49548: H2O + N(omega)-(1-hydroxy-2-oxopropyl)-L-arginyl-[protein] = H(+) + L-arginyl-[protein] + lactate
EC 3.5.1.124 |
RHEA:49552: H2O + N(6)-(1-hydroxy-2-oxopropyl)-L-lysyl-[protein] = H(+) + L-lysyl-[protein] + lactate
EC 3.5.1.124 |
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RHEA:49556: H2O + S-(1-hydroxy-2-oxopropyl)-L-cysteinyl-[protein] = H(+) + L-cysteinyl-[protein] + lactate
EC 3.5.1.124 |
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RHEA:57188: H2O + N(omega)-(1-hydroxy-2-oxoethyl)-L-arginyl-[protein] = glycolate + H(+) + L-arginyl-[protein] | |
RHEA:57192: H2O + N(6)-(1-hydroxy-2-oxoethyl)-L-lysyl-[protein] = glycolate + H(+) + L-lysyl-[protein] | |
RHEA:57196: H2O + S-(1-hydroxy-2-oxoethyl)-L-cysteinyl-[protein] = glycolate + H(+) + L-cysteinyl-[protein] | |
RHEA:57244: H2O + N(2)-(1-hydroxy-2-oxopropyl)-dGTP = dGTP + H(+) + lactate | |
RHEA:57256: H2O + N(2)-(1-hydroxy-2-oxopropyl)-GTP = GTP + H(+) + lactate | |
RHEA:57260: H2O + N(2)-(1-hydroxy-2-oxopropyl)-GDP = GDP + H(+) + lactate | |
RHEA:57268: H2O + N(2)-(1-hydroxy-2-oxopropyl)-GMP = GMP + H(+) + lactate | |
RHEA:57248: H2O + N(2)-(1-hydroxy-2-oxoethyl)-dGTP = dGTP + glycolate + H(+) | |
RHEA:57252: H2O + N(2)-(1-hydroxy-2-oxoethyl)-GTP = glycolate + GTP + H(+) | |
RHEA:57264: H2O + N(2)-(1-hydroxy-2-oxoethyl)-GDP = GDP + glycolate + H(+) | |
RHEA:57304: H2O + N(2)-(1-hydroxy-2-oxoethyl)-GMP = glycolate + GMP + H(+) | |
RHEA:57288: an N(2)-(1-hydroxy-2-oxopropyl)-guanosine in RNA + H2O = a guanosine in RNA + H(+) + lactate | |
RHEA:57300: an N(2)-(1-hydroxy-2-oxopropyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + H(+) + lactate | |
RHEA:57292: an N(2)-(1-hydroxy-2-oxoethyl)-guanosine in RNA + H2O = a guanosine in RNA + glycolate + H(+) | |
RHEA:57296: an N(2)-(1-hydroxy-2-oxoethyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + glycolate + H(+) |
case <OC:Enterobacterales>
Subunit | Homodimer. |
end case
Subcellular location | Cytoplasm. |
case <OC:Escherichia> or <OC:Shigella>
Induction | By heat shock. |
end case
Similarity | Belongs to the peptidase C56 family. HchA subfamily. |
Keywords
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case <FTGroup:1>
end case
Gene Ontology
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GO:0016790; Molecular function: thiolester hydrolase activity.
GO:0016811; Molecular function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides.
GO:0036524; Molecular function: protein deglycase activity.
GO:0016811; Molecular function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides.
GO:0036524; Molecular function: protein deglycase activity.
case <FTGroup:1>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0006281; Biological process: DNA repair.
GO:0030091; Biological process: protein repair.
GO:0005737; Cellular component: cytoplasm.
GO:0030091; Biological process: protein repair.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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Pfam | PF01965; DJ-1_PfpI; 1; |
PIRSF | PIRSF037798; Chaperone_HchA; 1; |
Features
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From: HCHA_ECOLI (P31658) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 185 | 185 | Nucleophile | C | ||||||||
BINDING (Optional) | 86 | 86 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | [H] | 1 | |||||||
BINDING (Optional) | 91 | 91 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | [E] | 1 | |||||||
BINDING (Optional) | 123 | 123 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | [H] | 1 |
Additional information
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Size range | 282-292 amino acids |
Related rules | None |
Fusion | None |