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HAMAP rule MF_01046

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General rule information [?]

Accession MF_01046
Dates 13-FEB-2003 (Created)
19-NOV-2022 (Last updated, Version 31)
Name Deglycase_HchA
Bacteria; Staphylococcus
Bacteria; Gammaproteobacteria
Template P31658 (HCHA_ECOLI)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Protein/nucleic acid deglycase HchA;
EC 3.1.2.-;
EC 3.5.1.-;
AltName: Full=Maillard deglycase;
Gene name

Comments [?]

Function Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of Schiff bases and advanced glycation endproducts (AGE). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Plays an important role in protecting cells from carbonyl stress.
Catalytic activity RHEA:49548: H2O + N(omega)-(1-hydroxy-2-oxopropyl)-L-arginyl-[protein] = H(+) + L-arginyl-[protein] + lactate
RHEA:49552: H2O + N(6)-(1-hydroxy-2-oxopropyl)-L-lysyl-[protein] = H(+) + L-lysyl-[protein] + lactate
RHEA:49556: H2O + S-(1-hydroxy-2-oxopropyl)-L-cysteinyl-[protein] = H(+) + L-cysteinyl-[protein] + lactate
RHEA:57188: H2O + N(omega)-(1-hydroxy-2-oxoethyl)-L-arginyl-[protein] = glycolate + H(+) + L-arginyl-[protein]
RHEA:57192: H2O + N(6)-(1-hydroxy-2-oxoethyl)-L-lysyl-[protein] = glycolate + H(+) + L-lysyl-[protein]
RHEA:57196: H2O + S-(1-hydroxy-2-oxoethyl)-L-cysteinyl-[protein] = glycolate + H(+) + L-cysteinyl-[protein]
RHEA:57244: H2O + N(2)-(1-hydroxy-2-oxopropyl)-dGTP = dGTP + H(+) + lactate
RHEA:57256: H2O + N(2)-(1-hydroxy-2-oxopropyl)-GTP = GTP + H(+) + lactate
RHEA:57260: H2O + N(2)-(1-hydroxy-2-oxopropyl)-GDP = GDP + H(+) + lactate
RHEA:57268: H2O + N(2)-(1-hydroxy-2-oxopropyl)-GMP = GMP + H(+) + lactate
RHEA:57248: H2O + N(2)-(1-hydroxy-2-oxoethyl)-dGTP = dGTP + glycolate + H(+)
RHEA:57252: H2O + N(2)-(1-hydroxy-2-oxoethyl)-GTP = glycolate + GTP + H(+)
RHEA:57264: H2O + N(2)-(1-hydroxy-2-oxoethyl)-GDP = GDP + glycolate + H(+)
RHEA:57304: H2O + N(2)-(1-hydroxy-2-oxoethyl)-GMP = glycolate + GMP + H(+)
RHEA:57288: an N(2)-(1-hydroxy-2-oxopropyl)-guanosine in RNA + H2O = a guanosine in RNA + H(+) + lactate
RHEA:57300: an N(2)-(1-hydroxy-2-oxopropyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + H(+) + lactate
RHEA:57292: an N(2)-(1-hydroxy-2-oxoethyl)-guanosine in RNA + H2O = a guanosine in RNA + glycolate + H(+)
RHEA:57296: an N(2)-(1-hydroxy-2-oxoethyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + glycolate + H(+)
case <OC:Enterobacterales>
Subunit Homodimer.
end case
Subcellular location Cytoplasm.
case <OC:Escherichia> or <OC:Shigella>
Induction By heat shock.
end case
Similarity Belongs to the peptidase C56 family. HchA subfamily.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

GO:0016790; Molecular function: thiolester hydrolase activity.
GO:0016811; Molecular function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides.
GO:0036524; Molecular function: protein deglycase activity.
case <FTGroup:1>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0006281; Biological process: DNA repair.
GO:0030091; Biological process: protein repair.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01965; DJ-1_PfpI; 1;
PIRSF PIRSF037798; Chaperone_HchA; 1;

Features [?]

From: HCHA_ECOLI (P31658)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     185     185       Nucleophile     C  
BINDING (Optional)     86     86       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     [H]   1
BINDING (Optional)     91     91       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     [E]   1
BINDING (Optional)     123     123       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     [H]   1

Additional information [?]

Size range 282-292 amino acids
Related rules None
Fusion None