HAMAP rule MF_01057
General rule information
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| Accession | MF_01057 |
| Dates | 12-JUN-2003 (Created)
13-OCT-2023 (Last updated, Version 31) |
| Name | tRNA_methyltr_TrmB |
| Scope(s) |
Bacteria |
| Template(s) | P0A8I5 (TRMB_ECOLI); O34522 (TRMB_BACSU); P67506 (TRMB_STRPN); O66479 (TRMB_AQUAE); [ Recover all ] |
| Triggered by |
HAMAP; MF_01057 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TRMB |
| Protein name | RecName: Full=tRNA (guanine-N(7)-)-methyltransferase; EC=2.1.1.33; AltName: Full=tRNA (guanine(46)-N(7))-methyltransferase; AltName: Full=tRNA(m7G46)-methyltransferase; |
| Gene name | Name=trmB; |
Comments
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| FUNCTION | Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. |
| CATALYTIC ACTIVITY | Reaction=guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)- methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:42708, Rhea:RHEA-COMP:10188, Rhea:RHEA-COMP:10189, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269, ChEBI:CHEBI:74480; EC=2.1.1.33; |
| PATHWAY | tRNA modification; N(7)-methylguanine-tRNA biosynthesis. |
| case <OC:Enterobacterales> | |
| SUBUNIT | Monomer. |
| end case | |
| SIMILARITY | Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family. |
Keywords
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Gene Ontology
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| GO:0008176; Molecular function:tRNA (guanine(46)-N7)-methyltransferase activity |
| GO:0006400; Biological process:tRNA modification |
Cross-references
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Features
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| From: TRMB_ECOLI (P0A8I5) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | 150 | 155 | /note="Interaction with RNA" | [KR]-[KH]-[EN]-K-R-R | ||||||||
| BINDING | 217 | 220 | /ligand="substrate" | T-x-[YF]-E | ||||||||
| BINDING | 69 | 69 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[DE] | ||||||||
| BINDING | 94 | 94 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[DE] | ||||||||
| BINDING | 121 | 121 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[DEN] | ||||||||
| BINDING | 144 | 144 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[DN] | ||||||||
| BINDING | 148 | 148 | /ligand="substrate" | K | ||||||||
| BINDING | 180 | 180 | /ligand="substrate" | D | ||||||||
Additional information
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| Size range | 185-400 amino acids |
| Related rules |
None |
| Fusion | Nter: hemK; kdtA Cter: None |
| Comments | Fused to hemK in RICBR, RICCN, RICFE, RICPR and RICTY, and fused to kdtA in FUSNN; Unknown N-terminal domain in METPP; sequences not included in alignment and not taken into account in size range |