HAMAP rule MF_01057

General rule information [?]

Accession	MF_01057
Dates	12-JUN-2003 (Created) 1-JUN-2023 (Last updated, Version 30)

Name	tRNA_methyltr_TrmB

Scope

Bacteria

Templates

P0A8I5 (TRMB_ECOLI); O34522 (TRMB_BACSU); P67506 (TRMB_STRPN); O66479 (TRMB_AQUAE): [Recover all]

Triggered by

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

TRMB

Protein name

RecName:		Full=tRNA (guanine-N(7)-)-methyltransferase;
		EC 2.1.1.33;
AltName:		Full=tRNA (guanine(46)-N(7))-methyltransferase;
AltName:		Full=tRNA(m7G46)-methyltransferase;

Gene name

trmB

Comments [?]

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.
Catalytic activity	RHEA:42708: guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine EC 2.1.1.33
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.

case <OC:Enterobacterales>

Subunit

Monomer.

end case

Similarity

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Keywords [?]

Gene Ontology [?]

GO:0008176; Molecular function: tRNA (guanine-N7-)-methyltransferase activity.
GO:0006400; Biological process: tRNA modification.

Cross-references [?]

Features [?]

From: TRMB_ECOLI (P0A8I5)

Key From To Description Tag Condition FTGroup

REGION (Optional) 150 155 Interaction with RNA [KR]-[KH]-[EN]-K-R-R

BINDING (Optional) 217 220 /ligand="substrate T-x-[YF]-E

BINDING (Optional) 69 69 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 [DE]

BINDING (Optional) 94 94 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 [DE]

BINDING (Optional) 121 121 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 [DEN]

BINDING (Optional) 144 144 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 [DN]

BINDING (Optional) 148 148 /ligand="substrate K

BINDING (Optional) 180 180 /ligand="substrate D

Additional information [?]

Size range	185-400 amino acids
Related rules	None
Fusion	Nter: hemK, kdtA; Cter: None
Comments	Fused to hemK in RICBR, RICCN, RICFE, RICPR and RICTY, and fused to kdtA in FUSNN; Unknown N-terminal domain in METPP; sequences not included in alignment and not taken into account in size range