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HAMAP rule MF_01057

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General rule information [?]

Accession MF_01057
Dates 12-JUN-2003 (Created)
13-OCT-2023 (Last updated, Version 31)
Name tRNA_methyltr_TrmB
Scope(s) Bacteria
Template(s) P0A8I5 (TRMB_ECOLI); O34522 (TRMB_BACSU); P67506 (TRMB_STRPN); O66479 (TRMB_AQUAE); [ Recover all ]
Triggered by HAMAP; MF_01057 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TRMB
Protein name RecName: Full=tRNA (guanine-N(7)-)-methyltransferase;
AltName: Full=tRNA (guanine(46)-N(7))-methyltransferase;
AltName: Full=tRNA(m7G46)-methyltransferase;
Gene name Name=trmB;

Comments [?]

FUNCTIONCatalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.
CATALYTIC ACTIVITY Reaction=guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)- methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:42708, Rhea:RHEA-COMP:10188, Rhea:RHEA-COMP:10189, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269, ChEBI:CHEBI:74480; EC=;
PATHWAYtRNA modification; N(7)-methylguanine-tRNA biosynthesis.
case <OC:Enterobacterales>
end case
SIMILARITYBelongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Keywords [?]

Gene Ontology [?]

GO:0008176; Molecular function:tRNA (guanine(46)-N7)-methyltransferase activity
GO:0006400; Biological process:tRNA modification

Cross-references [?]

Pfam PF02390; Methyltransf_4; 1;
NCBIfam TIGR00091; TIGR00091; 1;

Features [?]

Key From To Description Tag Condition FTGroup
REGION 150 155 /note="Interaction with RNA" [KR]-[KH]-[EN]-K-R-R
BINDING 217 220 /ligand="substrate" T-x-[YF]-E
BINDING 69 69 /ligand="S-adenosyl-L-methionine"
BINDING 94 94 /ligand="S-adenosyl-L-methionine"
BINDING 121 121 /ligand="S-adenosyl-L-methionine"
BINDING 144 144 /ligand="S-adenosyl-L-methionine"
BINDING 148 148 /ligand="substrate" K
BINDING 180 180 /ligand="substrate" D

Additional information [?]

Size range 185-400 amino acids
Related rules None
Fusion Nter: hemK; kdtA Cter: None
Comments Fused to hemK in RICBR, RICCN, RICFE, RICPR and RICTY, and fused to kdtA in FUSNN; Unknown N-terminal domain in METPP; sequences not included in alignment and not taken into account in size range

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