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Annotation rule MF_01106
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General rule information [?]

Accession MF_01106
Dates 28-MAR-2003 (Created)
19-NOV-2019 (Last updated, Version 36)
Name ArgJ
Scope
Bacteria
Archaea
Templates Q07908 (ARGJ_GEOSE); P9WPZ3 (ARGJ_MYCTU); Q9Z4S1 (ARGJ_THENN); P0DJQ5 (GNAT2_STRCL); Q9K8V3 (ARGJ_BACHD): [Recover all]
case <OC:Bacteria> or <OC:Archaea>
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ARGJ
Protein name
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ;
Includes:
RecName: Full=Glutamate N-acetyltransferase;
EC 2.3.1.35;
AltName: Full=Ornithine acetyltransferase;
Short=OATase;
AltName: Full=Ornithine transacetylase;
Includes:
RecName: Full=Amino-acid acetyltransferase;
EC 2.3.1.1;
AltName: Full=N-acetylglutamate synthase;
Short=AGSase;
Contains:
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ alpha chain;
Contains:
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ beta chain;
Gene name
argJ

Comments [?]

Function Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.
Catalytic activity RHEA:15349: L-glutamate + N(2)-acetyl-L-ornithine = L-ornithine + N-acetyl-L-glutamate
EC 2.3.1.35
RHEA:24292: acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate
EC 2.3.1.1
Pathway Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.
Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.
Subunit Heterotetramer of two alpha and two beta chains.
Subcellular location Cytoplasm.
Miscellaneous Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway.
Similarity Belongs to the ArgJ family.

Keywords [?]


Gene Ontology [?]

GO:0004042; Molecular function: acetyl-CoA:L-glutamate N-acetyltransferase activity.
GO:0004358; Molecular function: glutamate N-acetyltransferase activity.
GO:0006526; Biological process: arginine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01960; ArgJ; 1;
TIGRFAMs TIGR00120; ArgJ; 1;

Features [?]

From: ARGJ_MYCTU (P9WPZ3)
Key     From     To       Description   Tag   Condition   FTGroup
CHAIN     Nter     199       Arginine biosynthesis bifunctional protein ArgJ alpha chain        
CHAIN     200     Cter       Arginine biosynthesis bifunctional protein ArgJ beta chain        
ACT_SITE     200     200       Nucleophile     [TS]  
BINDING     166     166       Substrate     T  
BINDING     189     189       Substrate     K  
BINDING     200     200       Substrate     [TS]  
BINDING     280     280       Substrate     E  
BINDING     399     399       Substrate     N  
BINDING     404     404       Substrate     [ST]  
SITE     127     127       Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole     T  
SITE     128     128       Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole     G  
SITE     199     200       Cleavage; by autolysis     [AG]-[TS]  

Additional information [?]

Size range 379-432 amino acids
Related rules None
Fusion None