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HAMAP rule MF_01109

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General rule information [?]

Accession MF_01109
Dates 11-JUL-2003 (Created)
30-APR-2024 (Last updated, Version 41)
Name OTCase
Scope(s) Bacteria
Archaea
Template(s) P04391 (OTC1_ECOLI); P9WIT9 (OTC_MYCTU); Q8DCF5 (OTC_VIBVU); [ Recover all ]
Triggered by HAMAP; MF_01109 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case defined <Property:ARGININE_BS> and not <Property:ARGININE_BS>
Identifier OTCC
Protein name RecName: Full=Ornithine carbamoyltransferase, catabolic;
                 Short=OTCase;
                 EC=2.1.3.3;
else
Identifier OTC
Protein name RecName: Full=Ornithine carbamoyltransferase;
                 Short=OTCase;
                 EC=2.1.3.3;
end case
case <Property:ARGININE_BS> and <OC:Enterobacterales>
Gene name Name=argI;
end case
case <Property:ARGININE_BS> and not <OC:Enterobacterales>
Gene name Name=argF;
end case
case not <Property:ARGININE_BS>
Gene name Name=arcB;
end case

Comments [?]

CATALYTIC ACTIVITY Reaction=carbamoyl phosphate + L-ornithine = H(+) + L-citrulline + phosphate; Xref=Rhea:RHEA:19513, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474, ChEBI:CHEBI:46911, ChEBI:CHEBI:57743, ChEBI:CHEBI:58228; EC=2.1.3.3;
case <Property:ARGININE_BS>
PATHWAYAmino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.
end case
case not <Property:ARGININE_BS>
PATHWAYAmino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 2/2.
end case
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.

Keywords [?]

case <Property:ARGININE_BS>
Amino-acid biosynthesis
Arginine biosynthesis
end case
case not <Property:ARGININE_BS>
Arginine metabolism
end case
Cytoplasm
Transferase

Gene Ontology [?]

GO:0004585; Molecular function:ornithine carbamoyltransferase activity
case <Property:ARGININE_BS>
GO:0006526; Biological process:arginine biosynthetic process
end case
case not <Property:ARGININE_BS>
GO:0019547; Biological process:arginine catabolic process to ornithine
end case
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00185; OTCace; 1;
Pfam PF02729; OTCace_N; 1;
PRINTS PR00100; AOTCASE; 1;
NCBIfam TIGR00658; Orni_carb_tr; 1;
PROSITE PS00097; CARBAMOYLTRANSFERASE; 1;

Features [?]

From: OTC1_ECOLI (P04391)
Key From To Description Tag Condition FTGroup
BINDING 56 59 /ligand="carbamoyl phosphate"
/ligand_id="ChEBI:CHEBI:58228"
S-[TS]-R-T
BINDING 134 137 /ligand="carbamoyl phosphate"
/ligand_id="ChEBI:CHEBI:58228"
H-x(2)-Q
BINDING 236 237 /ligand="L-ornithine"
/ligand_id="ChEBI:CHEBI:46911"
S-[MIL]
BINDING 274 275 /ligand="carbamoyl phosphate"
/ligand_id="ChEBI:CHEBI:58228"
C-L
BINDING 83 83 /ligand="carbamoyl phosphate"
/ligand_id="ChEBI:CHEBI:58228"
[QN]
BINDING 107 107 /ligand="carbamoyl phosphate"
/ligand_id="ChEBI:CHEBI:58228"
R
BINDING 168 168 /ligand="L-ornithine"
/ligand_id="ChEBI:CHEBI:46911"
N
BINDING 232 232 /ligand="L-ornithine"
/ligand_id="ChEBI:CHEBI:46911"
D
BINDING 320 320 /ligand="carbamoyl phosphate"
/ligand_id="ChEBI:CHEBI:58228"
[RK]

Additional information [?]

Size range 295-354 amino acids
Related rules MF_02102
Fusion Nter: None Cter: None
Comments OTCases can be involved in the biosynthesis of arginine and in its catabolism, by the arginine deiminase pathway. Some bacteria possess two distinct OTCases; some do not degrade arginine using this pathway and others do not synthesize arginine. As it is impossible to distinguish between anabolic and catabolic OTCases based on sequence similarity alone, if paralogs are found in a given genome, then one is probably the anabolic enzyme and the other the catabolic one, named arcB, which is usually located in the arcABC operon. E.coli K-12 carries two genes for anabolic OTCase, argF and argI; their products interact to form a family of four trimeric isoenzymes. Only argI can be found in E.coli B, E.coli W or in other enterobacteria.



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