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HAMAP rule MF_01109

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General rule information [?]

Accession MF_01109
Dates 11-JUL-2003 (Created)
1-JUN-2023 (Last updated, Version 40)
Name OTCase
Scope
Bacteria
Archaea
Templates P04391 (OTC1_ECOLI); P9WIT9 (OTC_MYCTU); Q8DCF5 (OTC_VIBVU): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

case defined <Property:ARGININE_BS> and not <Property:ARGININE_BS>
Identifier
OTCC
Protein name
RecName: Full=Ornithine carbamoyltransferase, catabolic;
Short=OTCase;
EC 2.1.3.3;
else
Identifier
OTC
Protein name
RecName: Full=Ornithine carbamoyltransferase;
Short=OTCase;
EC 2.1.3.3;
end case
case <Property:ARGININE_BS> and <OC:Enterobacterales>
Gene name
argI
end case
case <Property:ARGININE_BS> and not <OC:Enterobacterales>
Gene name
argF
end case
case not <Property:ARGININE_BS>
Gene name
arcB
end case

Comments [?]

Catalytic activity RHEA:19513: carbamoyl phosphate + L-ornithine = H(+) + L-citrulline + phosphate
EC 2.1.3.3
case <Property:ARGININE_BS>
Pathway Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.
end case
case not <Property:ARGININE_BS>
Pathway Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 2/2.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.

Keywords [?]

case <Property:ARGININE_BS>
Amino-acid biosynthesis
Arginine biosynthesis
end case
case not <Property:ARGININE_BS>
Arginine metabolism
end case
Cytoplasm
Transferase

Gene Ontology [?]

GO:0004585; Molecular function: ornithine carbamoyltransferase activity.
case <Property:ARGININE_BS>
GO:0006526; Biological process: arginine biosynthetic process.
end case
case not <Property:ARGININE_BS>
GO:0019546; Biological process: arginine deiminase pathway.
end case
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00185; OTCace; 1;
PF02729; OTCace_N; 1;
PRINTS PR00100; AOTCASE; 1;
NCBIfam TIGR00658; Orni_carb_tr; 1;
PROSITE PS00097; CARBAMOYLTRANSFERASE; 1;

Features [?]

From: OTC1_ECOLI (P04391)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     56     59       /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228     S-[TS]-R-T  
BINDING     134     137       /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228     H-x(2)-Q  
BINDING     236     237       /ligand="L-ornithine" /ligand_id="ChEBI:CHEBI:46911     S-[MIL]  
BINDING     274     275       /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228     C-L  
BINDING     83     83       /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228     [QN]  
BINDING     107     107       /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228     R  
BINDING     168     168       /ligand="L-ornithine" /ligand_id="ChEBI:CHEBI:46911     N  
BINDING     232     232       /ligand="L-ornithine" /ligand_id="ChEBI:CHEBI:46911     D  
BINDING     320     320       /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228     [RK]  

Additional information [?]

Size range 295-354 amino acids
Related rules MF_02102 (PTC supersedes the current rule)
Fusion None
Comments OTCases can be involved in the biosynthesis of arginine and in its catabolism, by the arginine deiminase pathway. Some bacteria possess two distinct OTCases; some do not degrade arginine using this pathway and others do not synthesize arginine. As it is impossible to distinguish between anabolic and catabolic OTCases based on sequence similarity alone, if paralogs are found in a given genome, then one is probably the anabolic enzyme and the other the catabolic one, named arcB, which is usually located in the arcABC operon. E.coli K-12 carries two genes for anabolic OTCase, argF and argI; their products interact to form a family of four trimeric isoenzymes. Only argI can be found in E.coli B, E.coli W or in other enterobacteria.


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