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Annotation rule MF_01121
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General rule information [?]

Accession MF_01121
Dates 28-OCT-2003 (Created)
19-NOV-2019 (Last updated, Version 34)
Name Sirtuin_ClassIII
Scope
Bacteria
Archaea
Templates O28597 (NPD1_ARCFU); Q9NXA8 (SIR5_HUMAN): [Recover all]
case <OC:Bacteria> or <OC:Archaea>
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
NPD
Protein name
RecName: Full=NAD-dependent protein deacylase;
EC 2.3.1.286;
AltName: Full=Regulatory protein SIR2 homolog;
Gene name
cobB

Comments [?]

case <OC:Bacteria> and <FTGroup:3>
Function NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form.
else case <OC:Archaea> and <FTGroup:3>
Function NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form. Deacetylates the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription.
else case <OC:Bacteria> and not <FTGroup:3>
Function NAD-dependent protein deacetylase which modulates the activities of several proteins which are inactive in their acetylated form.
else case <OC:Archaea> and not <FTGroup:3>
Function NAD-dependent protein deacetylase which modulates the activities of several proteins which are inactive in their acetylated form. Deacetylates the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription.
end case
Catalytic activity RHEA:43636: H2O + N(6)-acetyl-L-lysyl-[protein] + NAD(+) = 2''-O-acetyl-ADP-D-ribose + L-lysyl-[protein] + nicotinamide
EC 2.3.1.286
case <FTGroup:3>
Catalytic activity RHEA:47668: H2O + N(6)-succinyl-L-lysyl-[protein] + NAD(+) = 2''-O-succinyl-ADP-D-ribose + L-lysyl-[protein] + nicotinamide
end case
case <FTGroup:1>
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
end case
Subcellular location Cytoplasm.
case <FTGroup:3>
Domain 2 residues (#{Tyr-64} and #{Arg-67}) present in a large hydrophobic pocket are probably involved in substrate specificity. They are important for desuccinylation activity, but dispensable for deacetylation activity.
end case
Similarity Belongs to the sirtuin family. Class III subfamily.

Keywords [?]

case <FTGroup:1>
end case
case <OC:Archaea>
end case

Gene Ontology [?]

GO:0005737; Cellular component: cytoplasm.
GO:0034979; Molecular function: NAD-dependent protein deacetylase activity.
GO:0070403; Molecular function: NAD+ binding.
GO:0006476; Biological process: protein deacetylation.
case <FTGroup:3>
GO:0036055; Molecular function: protein-succinyllysine desuccinylase activity.
GO:0036048; Biological process: protein desuccinylation.
end case
case <FTGroup:1>
GO:0008270; Molecular function: zinc ion binding.
end case

Cross-references [?]

PROSITE PS50305; SIRTUIN; 1; trigger=PRU00236;
Pfam PF02146; SIR2; 1;

Features [?]

From: NPD1_ARCFU (O28597)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     20     39       NAD     G-A-G-x-S-[AK]-x-S-G-[ILV]-x-T-x(7,8)-W  
NP_BIND     98     101       NAD     Q-N-[IV]-[DE]  
NP_BIND     185     187       NAD     G-[TS]-S  
NP_BIND (Optional)     211     213       NAD     N-x(2)  
ACT_SITE     116     116       Proton acceptor     H  
METAL (Optional)     124     124       Zinc     C   1
METAL (Optional)     127     127       Zinc     C   2
METAL (Optional)     145     145       Zinc     C   1
METAL (Optional)     148     148       Zinc     C   2
BINDING (Optional)     64     64       Substrate     Y   3
BINDING (Optional)     67     67       Substrate     R   3
BINDING     229     229       NAD; via amide nitrogen        

Additional information [?]

Size range 208-262 amino acids
Related rules MF_01967 (NPD); MF_01968 (NPD)
Fusion None
Comments The gene name sir2 is used in a few prokaryotes. The gene is subject to alternative promoter usage in Salty, giving rise to 2 proteins with different N-termini. The longer protein is used in the seed alignment. Other Enterobacteriaceae probably do that too, maybe other bacteria as well.