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Annotation rule MF_01125 |
General rule information
[?]
Accession |
MF_01125 |
Dates |
31-JAN-2004 (Created) 19-NOV-2019 (Last updated, Version 27) |
Propagated annotation
[?]
Identifier, protein and gene names
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Protein name |
RecName:
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Full=Reverse gyrase;
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Includes:
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RecName:
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Full=Helicase;
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EC 3.6.4.12;
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Includes:
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RecName:
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Full=Topoisomerase;
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EC 5.6.2.2;
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Function |
Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process. It cleaves transiently a single DNA strand and remains covalently bound to the 5' DNA end through a tyrosine residue. May be involved in rewinding the DNA strands in the regions of the chromosome that have opened up to allow transcription or replication. |
Catalytic activity |
RHEA:13065: ATP + H2O = ADP + H(+) + phosphate
EC 3.6.4.12
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Reaction=ATP-dependent breakage, passage and rejoining of double-stranded DNA.; EC=5.6.2.2; |
Subunit |
Monomer. |
Domain |
Both the DNA unwinding and positive supercoiling activities require the cooperation of both domains. The cooperative action between the helicase-like and the topoisomerase domains is specific. The helicase-like domain probably does not directly unwind DNA but acts more likely by driving ATP-dependent conformational changes within the whole enzyme, functioning more like a protein motor. The 'latch' region of the N-terminal domain plays a regulatory role in the enzyme, repressing topoisomerase activity in the absence of ATP and therefore preventing the enzyme from acting as an ATP-independent relaxing enzyme; it also helps to coordinate nucleotide hydrolysis by the ATPase domain with the supercoiling activity of the topoisomerase domain. |
Miscellaneous |
This enzyme is the only unique feature of hyperthermophilic bacteria/archaea discovered so far. It appears to be essential for adaptation to life at high temperatures. |
Similarity |
In the N-terminal section; belongs to the DEAD box helicase family. DDVD subfamily. |
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In the C-terminal section; belongs to the prokaryotic type I/III topoisomerase family. |
case <FT:2> or <FT:4>
end case
GO:0005524; Molecular function: ATP binding.
GO:0003677; Molecular function: DNA binding.
GO:0003918; Molecular function: DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity.
GO:0004386; Molecular function: helicase activity.
case <FT:2> or <FT:4>
end case
GO:0006265; Biological process: DNA topological change.
GO:0006268; Biological process: DNA unwinding involved in DNA replication.
From: RGYR_PYRFU (P95479) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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ZN_FING
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9
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30
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C4-type 1
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C-x-x-C-x(10,18)-[CH]-x-x-C
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NP_BIND
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106
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113
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ATP
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x-P-x-G-x-G-K-[ST]
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ZN_FING
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722
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741
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C4-type 2
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C-x-x-C-x(10,18)-C-x-x-C
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REGION
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645
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Cter
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Topoisomerase I
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ACT_SITE
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955
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955
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For DNA cleavage activity
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Y
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BINDING
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89
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89
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ATP
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Q
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Additional information
[?]
Size range |
1054-1624 amino acids |
Related rules |
None |
Fusion |
None |
Comments |
The reverse gyrases of M. jannaschii and P. horikoshii contain inteins which were excised in the alignment |