 |
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| Annotation rule MF_01128 |
General rule information
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| Accession | MF_01128 |
| Dates | 22-APR-2003 (Created) 19-NOV-2019 (Last updated, Version 34) |
| Name | CLC_ClcA |
| Scope | Bacteria |
| Template | P37019 (CLCA_ECOLI) |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
|
| Protein name |
|
||||
case <OC:Enterobacterales>
| Gene name |
|
else
| Gene name |
|
end case
Comments
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| Function | Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response. |
| Subunit | Homodimer. |
case not defined <Property:Membrane> or <Property:Membrane=1>
| Subcellular location | Cell membrane; Multi-pass membrane protein. |
else case <Property:Membrane=2>
| Subcellular location | Cell inner membrane; Multi-pass membrane protein. |
end case
| Similarity | Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily. |
Keywords
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case defined <Property:Membrane> and <Property:Membrane=2>
end case
Gene Ontology
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GO:0015297; Molecular function: antiporter activity.
GO:0006821; Biological process: chloride transport.
GO:0005886; Cellular component: plasma membrane.
GO:0006821; Biological process: chloride transport.
GO:0005886; Cellular component: plasma membrane.
Cross-references
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Computed features
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case not defined <Property:Membrane> or <Property:Membrane=1>
| General | Transmembrane; -; 11; trigger=yes; |
end case
Features
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case defined <Property:Membrane> and <Property:Membrane=2>
| From: CLCA_ECOLI (P37019) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| TOPO_DOM | Nter | 32 | Cytoplasmic | |||||||||
| TRANSMEM | 33 | 69 | Helical | |||||||||
| TOPO_DOM | 70 | 76 | Periplasmic | |||||||||
| TRANSMEM | 77 | 100 | Helical | |||||||||
| INTRAMEM | 109 | 116 | Helical | |||||||||
| TOPO_DOM | 117 | 123 | Cytoplasmic | |||||||||
| TRANSMEM | 124 | 141 | Helical | |||||||||
| TRANSMEM | 148 | 166 | Helical | |||||||||
| TOPO_DOM | 167 | 176 | Cytoplasmic | |||||||||
| INTRAMEM | 177 | 189 | Helical | |||||||||
| INTRAMEM | 193 | 201 | Helical | |||||||||
| TOPO_DOM | 202 | 214 | Cytoplasmic | |||||||||
| TRANSMEM | 215 | 232 | Helical | |||||||||
| TOPO_DOM | 233 | 252 | Periplasmic | |||||||||
| TRANSMEM | 253 | 281 | Helical | |||||||||
| TOPO_DOM | 282 | 287 | Cytoplasmic | |||||||||
| TRANSMEM | 288 | 309 | Helical | |||||||||
| TOPO_DOM | 310 | 329 | Periplasmic | |||||||||
| TRANSMEM | 330 | 349 | Helical | |||||||||
| TRANSMEM | 355 | 376 | Helical | |||||||||
| TOPO_DOM | 377 | 386 | Periplasmic | |||||||||
| INTRAMEM | 387 | 401 | Helical | |||||||||
| INTRAMEM | 402 | 404 | Note=Loop between two helices | |||||||||
| INTRAMEM | 405 | 416 | Helical | |||||||||
| INTRAMEM | 417 | 421 | Note=Loop between two helices | |||||||||
| TRANSMEM | 422 | 438 | Helical | |||||||||
| TOPO_DOM | 439 | Cter | Cytoplasmic | |||||||||
| MOTIF | 106 | 110 | Selectivity filter part_1 | |||||||||
| MOTIF | 146 | 150 | Selectivity filter part_2 | |||||||||
| MOTIF | 355 | 359 | Selectivity filter part_3 | |||||||||
end case
Additional information
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| Size range | 467-478 amino acids |
| Related rules | None |
| Fusion | None |
| Comments | ClcA from E.coli was originally characterized as a voltage-gated chloride channel. It has now become clear that it is, in fact, a transporter. E.coli has at least one paralog, ClcB, and maybe YfeO, for which there is no electrophysiological characterization - they may or may not be transporters too. This family of proteins with high similarity to ClcA have, therefore, been considered as transporters as well. Further characterization is needed to distinguish ion channels from transporters, as it seems that the structural boundary separating channels and transporters is not as clear-cut as generally thought. |