HAMAP rule MF_01133
General rule information
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| Accession | MF_01133 |
| Dates | 25-SEP-2003 (Created)
1-JUN-2023 (Last updated, Version 35) |
| Name | RuBisCO_L_type3 |
| Scope(s) |
Archaea |
| Template(s) | O93627 (RBL_THEKO); Q58632 (RBL_METJA); O28635 (RBL_ARCFU); Q8THG2 (RBL_METAC); [ Recover all ] |
| Triggered by |
HAMAP; MF_01133 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RBL |
| Protein name | RecName: Full=Ribulose bisphosphate carboxylase; Short=RuBisCO; EC=4.1.1.39; |
| Gene name | Name=rbcL; |
Comments
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| FUNCTION | Catalyzes the addition of molecular CO(2) and H(2)O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3- phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase. |
| CATALYTIC ACTIVITY | Reaction=2 (2R)-3-phosphoglycerate + 2 H(+) = CO2 + D-ribulose 1,5- bisphosphate + H2O; Xref=Rhea:RHEA:23124, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57870, ChEBI:CHEBI:58272; EC=4.1.1.39; |
| CATALYTIC ACTIVITY | Reaction=D-ribulose 1,5-bisphosphate + O2 = (2R)-3-phosphoglycerate + 2-phosphoglycolate + 2 H(+); Xref=Rhea:RHEA:36631, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:57870, ChEBI:CHEBI:58033, ChEBI:CHEBI:58272; |
| case <FTGroup:1> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
| end case | |
| SUBUNIT | Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits. |
| MISCELLANEOUS | Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O(2) to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains. |
| SIMILARITY | Belongs to the RuBisCO large chain family. Type III subfamily. |
Keywords
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| Carbon dioxide fixation | |
| Lyase | |
| Oxidoreductase | |
| case <FTGroup:1> | |
| Magnesium | |
| Metal-binding | |
| end case | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
| GO:0016984; Molecular function:ribulose-bisphosphate carboxylase activity | |
| GO:0006196; Biological process:AMP catabolic process | |
Cross-references
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| Pfam | PF00016; RuBisCO_large; 1; |
| Pfam | PF02788; RuBisCO_large_N; 1; |
| NCBIfam | TIGR03326; rubisco_III; 1; |
Features
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| From: RBL_THEKO (O93627) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 367 | 369 | /ligand="substrate" | S-x-G | ||||||||
| BINDING | 389 | 392 | /ligand="substrate" | Q-x-G-G | ||||||||
| ACT_SITE | 163 | 163 | /note="Proton acceptor" | K | ||||||||
| ACT_SITE | 281 | 281 | /note="Proton acceptor" | H | ||||||||
| BINDING | 189 | 189 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /note="via carbamate group" |
K | 1 | |||||||
| BINDING | 191 | 191 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 192 | 192 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | 1 | |||||||
| BINDING | 165 | 165 | /ligand="substrate" | K | ||||||||
| BINDING | 282 | 282 | /ligand="substrate" | R | ||||||||
| BINDING | 314 | 314 | /ligand="substrate" | H | ||||||||
| SITE | 322 | 322 | /note="Transition state stabilizer" | K | ||||||||
| MOD_RES | 189 | 189 | /note="N6-carboxylysine" | K | ||||||||
Additional information
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| Size range | 410-450 amino acids |
| Related rules |
MF_01338 MF_01339 |
| Fusion | Nter: None Cter: None |