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HAMAP rule MF_01152

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General rule information [?]

Accession MF_01152
Dates 12-APR-2005 (Created)
1-JUN-2023 (Last updated, Version 25)
Name DnaJ
Scope
Bacteria
Archaea; Euryarchaeota
Template P08622 (DNAJ_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
DNAJ
Protein name
RecName: Full=Chaperone protein DnaJ;
Gene name
dnaJ

Comments [?]

Function Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.
case <FTGroup:2> or <FTGroup:3>
Cofactor Zn(2+)
Note: Binds 2 Zn(2+) ions per monomer.
end case
Subunit Homodimer.
Subcellular location Cytoplasm.
Domain The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.
Similarity Belongs to the DnaJ family.

Keywords [?]

Cytoplasm
Chaperone
DNA replication
Stress response
case <FTGroup:2> or <FTGroup:3>
Metal-binding
end case
Repeat
case <FTGroup:2> or <FTGroup:3>
Zinc
end case
case <Feature:PS51188>
Zinc-finger
end case

Gene Ontology [?]

GO:0051082; Molecular function: unfolded protein binding.
case <FTGroup:2> or <FTGroup:3>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00226; DnaJ; 1;
PF01556; DnaJ_C; 1;
PF00684; DnaJ_CXXCXGXG; 1;
PRINTS PR00625; JDOMAIN; 1;
PROSITE PS00636; DNAJ_1; 1;
PS50076; DNAJ_2; 1; trigger=PRU00286;
PS51188; ZF_CR; 1; trigger=PRU00546;
NCBIfam TIGR02349; DnaJ_bact; 1;

Features [?]

From: DNAJ_ECOLI (P08622)
Key     From     To       Description   Tag   Condition   FTGroup
REPEAT     144     151       CXXCXGXG motif     C-x(2)-C-x-G-x-G   1
REPEAT     161     168       CXXCXGXG motif     C-x(2)-C-x-G-x-G   1
REPEAT     183     190       CXXCXGXG motif     C-x(2)-C-x-G-x-G   1
REPEAT     197     204       CXXCXGXG motif     C-x(2)-C-x-G-x-G   1
BINDING     144     144       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     C   2
BINDING     147     147       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     C   2
BINDING     161     161       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     C   3
BINDING     164     164       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     C   3
BINDING     183     183       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     C   3
BINDING     186     186       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     C   3
BINDING     197     197       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     C   2
BINDING     200     200       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     C   2

Additional information [?]

Size range 347-403 amino acids
Related rules None
Fusion None


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