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Annotation rule MF_01152 |
General rule information
[?]
Accession |
MF_01152 |
Dates |
12-APR-2005 (Created) 19-NOV-2019 (Last updated, Version 21) |
Scope |
Bacteria
Archaea; Euryarchaeota |
Propagated annotation
[?]
Identifier, protein and gene names
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Protein name |
RecName:
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Full=Chaperone protein DnaJ;
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Function |
Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins. |
case <FTGroup:2> or <FTGroup:3>
Cofactor |
Zn(2+) Note: Binds 2 Zn(2+) ions per monomer. |
end case
Subunit |
Homodimer. |
Subcellular location |
Cytoplasm. |
Domain |
The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity. |
Similarity |
Belongs to the DnaJ family. |
case <FTGroup:2> or <FTGroup:3>
end case
case <FTGroup:2> or <FTGroup:3>
end case
case <Feature:PS51188>
end case
GO:0051082; Molecular function: unfolded protein binding.
case <FTGroup:2> or <FTGroup:3>
end case
From: DNAJ_ECOLI (P08622) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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REPEAT
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144
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151
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CXXCXGXG motif
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C-x(2)-C-x-G-x-G
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1
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REPEAT
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161
|
|
168
|
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CXXCXGXG motif
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|
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C-x(2)-C-x-G-x-G
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1
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REPEAT
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183
|
|
190
|
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CXXCXGXG motif
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C-x(2)-C-x-G-x-G
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1
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REPEAT
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197
|
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204
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CXXCXGXG motif
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C-x(2)-C-x-G-x-G
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1
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COMPBIAS
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77
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114
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Gly-rich
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METAL
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144
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144
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Zinc 1
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C
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2
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METAL
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147
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147
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Zinc 1
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|
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C
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2
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METAL
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161
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161
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Zinc 2
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C
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3
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METAL
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164
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164
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Zinc 2
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|
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C
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3
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METAL
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183
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183
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Zinc 2
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|
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C
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3
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METAL
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186
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186
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Zinc 2
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|
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C
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3
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METAL
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197
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197
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Zinc 1
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|
|
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C
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2
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METAL
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200
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|
200
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Zinc 1
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C
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2
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Additional information
[?]
Size range |
347-403 amino acids |
Related rules |
None |
Fusion |
None |