HAMAP rule MF_01161
General rule information
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| Accession | MF_01161 |
| Dates | 1-MAR-2005 (Created)
3-SEP-2024 (Last updated, Version 31) |
| Name | tRNA_Ile_lys_synt |
| Scope(s) |
Bacteria Plastid |
| Template(s) | P52097 (TILS_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01161 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TILS |
| case not <OG:Chloroplast> | |
| Protein name | RecName: Full=tRNA(Ile)-lysidine synthase; EC=6.3.4.19; AltName: Full=tRNA(Ile)-2-lysyl-cytidine synthase; AltName: Full=tRNA(Ile)-lysidine synthetase; |
| else case <OG:Chloroplast> | |
| Protein name | RecName: Full=tRNA(Ile)-lysidine synthase, chloroplastic; EC=6.3.4.19; AltName: Full=tRNA(Ile)-2-lysyl-cytidine synthase; AltName: Full=tRNA(Ile)-lysidine synthetase; |
| end case | |
| Gene name | Name=tilS; |
Comments
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| FUNCTION | Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. |
| CATALYTIC ACTIVITY | Reaction=cytidine(34) in tRNA(Ile2) + L-lysine + ATP = lysidine(34) in tRNA(Ile2) + AMP + diphosphate + H(+); Xref=Rhea:RHEA:43744, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10670, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019, ChEBI:CHEBI:82748, ChEBI:CHEBI:83665, ChEBI:CHEBI:456215; EC=6.3.4.19; |
| case <OC:Bacteria> | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| else case <OG:Chloroplast> | |
| SUBCELLULAR LOCATION | Plastid, chloroplast. |
| end case | |
| case <FT:1> | |
| DOMAIN | The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. |
| end case | |
| SIMILARITY | Belongs to the tRNA(Ile)-lysidine synthase family. |
Keywords
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| case <FT:1> | |
| ATP-binding | |
| Nucleotide-binding | |
| end case | |
| case <OC:Bacteria> | |
| Cytoplasm | |
| end case | |
| Ligase | |
| tRNA processing | |
Gene Ontology
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| case <FT:1> | |
| GO:0005524; Molecular function:ATP binding | |
| end case | |
| GO:0016879; Molecular function:ligase activity, forming carbon-nitrogen bonds | |
| GO:0006400; Biological process:tRNA modification | |
| case <OCellular component:Bacteria> | |
| GO:0005737; Cellular component:cytoplasm | |
| else case <OG:Chloroplast> | |
| GO:0009507; Cellular component:chloroplast | |
| end case | |
Cross-references
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| Pfam | PF01171; ATP_bind_3; 1; |
| NCBIfam | TIGR02433; lysidine_TilS_C; 1; |
| NCBIfam | TIGR02432; lysidine_TilS_N; 1; |
Features
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| From: TILS_ECOLI (P52097) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 20 | 25 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
S-G-G-x-D-S | ||||||||
Additional information
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| Size range | 278-648 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: <hprT>; <CMP/dCMP deaminase zinc-binding domain> |