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HAMAP rule MF_01161

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General rule information [?]

Accession MF_01161
Dates 1-MAR-2005 (Created)
1-JUN-2023 (Last updated, Version 30)
Name tRNA_Ile_lys_synt
Scope(s) Bacteria
Template(s) P52097 (TILS_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01161 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TILS
case not <OG:Chloroplast>
Protein name RecName: Full=tRNA(Ile)-lysidine synthase;
AltName: Full=tRNA(Ile)-2-lysyl-cytidine synthase;
AltName: Full=tRNA(Ile)-lysidine synthetase;
else case <OG:Chloroplast>
Protein name RecName: Full=tRNA(Ile)-lysidine synthase, chloroplastic;
AltName: Full=tRNA(Ile)-2-lysyl-cytidine synthase;
AltName: Full=tRNA(Ile)-lysidine synthetase;
end case
Gene name Name=tilS;

Comments [?]

FUNCTIONLigates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.
CATALYTIC ACTIVITY Reaction=ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP + diphosphate + H(+) + lysidine(34) in tRNA(Ile2); Xref=Rhea:RHEA:43744, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10670, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019, ChEBI:CHEBI:82748, ChEBI:CHEBI:83665, ChEBI:CHEBI:456215; EC=;
case <OC:Bacteria>
else case <OG:Chloroplast>
SUBCELLULAR LOCATIONPlastid, chloroplast.
end case
case <FT:1>
DOMAINThe N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.
end case
SIMILARITYBelongs to the tRNA(Ile)-lysidine synthase family.

Keywords [?]

case <FT:1>
end case
case <OC:Bacteria>
end case
tRNA processing

Gene Ontology [?]

case <FT:1>
GO:0005524; Molecular function:ATP binding
end case
GO:0016879; Molecular function:ligase activity, forming carbon-nitrogen bonds
GO:0006400; Biological process:tRNA modification
case <OCellular component:Bacteria>
GO:0005737; Cellular component:cytoplasm
else case <OG:Chloroplast>
GO:0009507; Cellular component:chloroplast
end case

Cross-references [?]

Pfam PF01171; ATP_bind_3; 1;
NCBIfam TIGR02433; lysidine_TilS_C; 1;
NCBIfam TIGR02432; lysidine_TilS_N; 1;

Features [?]

From: TILS_ECOLI (P52097)
Key From To Description Tag Condition FTGroup
BINDING 20 25 /ligand="ATP"

Additional information [?]

Size range 278-648 amino acids
Related rules None
Fusion Nter: None Cter: <hprT>; <CMP/dCMP deaminase zinc-binding domain>

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