HAMAP rule MF_01162
General rule information
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| Accession | MF_01162 |
| Dates | 11-APR-2005 (Created)
19-NOV-2019 (Last updated, Version 33) |
| Name | Aas |
| Scope(s) |
Bacteria Enterobacterales |
| Template(s) | P31119 (AAS_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01162 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | AAS |
| Protein name | RecName: Full=Bifunctional protein Aas; Includes: RecName: Full=2-acylglycerophosphoethanolamine acyltransferase; EC=2.3.1.40; AltName: Full=2-acyl-GPE acyltransferase; AltName: Full=Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferase; Includes: RecName: Full=Acyl-[acyl-carrier-protein] synthetase; EC=6.2.1.20; AltName: Full=Acyl-ACP synthetase; AltName: Full=Long-chain-fatty-acid--[acyl-carrier-protein] ligase; |
| Gene name | Name=aas; |
Comments
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| FUNCTION | Plays a role in lysophospholipid acylation. Transfers fatty acids to the 1-position via an enzyme-bound acyl-ACP intermediate in the presence of ATP and magnesium. Its physiological function is to regenerate phosphatidylethanolamine from 2-acyl-glycero-3- phosphoethanolamine (2-acyl-GPE) formed by transacylation reactions or degradation by phospholipase A1. |
| CATALYTIC ACTIVITY | Reaction=a 2-acyl-sn-glycero-3-phosphoethanolamine + a fatty acyl-[ACP] = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + holo-[ACP]; Xref=Rhea:RHEA:10304, Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:14125, ChEBI:CHEBI:64479, ChEBI:CHEBI:64612, ChEBI:CHEBI:65213, ChEBI:CHEBI:138651; EC=2.3.1.40; |
| CATALYTIC ACTIVITY | Reaction=a long-chain fatty acid + ATP + holo-[ACP] = a long-chain fatty acyl-[ACP] + AMP + diphosphate; Xref=Rhea:RHEA:45588, Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:12682, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57560, ChEBI:CHEBI:64479, ChEBI:CHEBI:133243, ChEBI:CHEBI:456215; EC=6.2.1.20; |
| case not defined <Property:Membrane> or <Property:Membrane=1> | |
| SUBCELLULAR LOCATION | Cell membrane; Multi-pass membrane protein. |
| else case <Property:Membrane=2> | |
| SUBCELLULAR LOCATION | Cell inner membrane; Multi-pass membrane protein. |
| end case | |
| SIMILARITY | In the N-terminal section; belongs to the 2-acyl-GPE acetyltransferase family. |
| SIMILARITY | In the C-terminal section; belongs to the ATP-dependent AMP-binding enzyme family. |
Keywords
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| Acyltransferase | |
| ATP-binding | |
| Nucleotide-binding | |
| case defined <Property:Membrane> and <Property:Membrane=2> | |
| Cell inner membrane | |
| end case | |
| Cell membrane | |
| Ligase | |
| Membrane | |
| Multifunctional enzyme | |
| Transferase | |
| Transmembrane | |
| Transmembrane helix | |
Gene Ontology
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| GO:0008779; Molecular function:acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase activity |
| GO:0008922; Molecular function:long-chain fatty acid [acyl-carrier-protein] ligase activity |
| GO:0005886; Cellular component:plasma membrane |
Cross-references
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| Pfam | PF01553; Acyltransferase; 1; |
| Pfam | PF00501; AMP-binding; 1; |
| PRINTS | PR00154; AMPBINDING; 1; |
| PROSITE | PS00455; AMP_BINDING; 1; |
Features
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| From: AAS_ECOLI (P31119) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| TRANSMEM | 258 | 277 | /note="Helical" | |||||||||
| TRANSMEM | 409 | 433 | /note="Helical" | |||||||||
| REGION | 15 | 138 | /note="Acyltransferase" | |||||||||
| REGION | 233 | 646 | /note="AMP-binding" | |||||||||
| ACT_SITE | 36 | 36 | H | |||||||||
Additional information
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| Size range | 718-738 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |