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Annotation rule MF_01166
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General rule information [?]

Accession MF_01166
Dates 11-NOV-2005 (Created)
19-NOV-2019 (Last updated, Version 28)
Name ArnA
Scope
Bacteria; Gammaproteobacteria
Templates P77398 (ARNA_ECOLI); O52325 (ARNA_SALTY): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ARNA
Protein name
RecName: Full=Bifunctional polymyxin resistance protein ArnA;
Includes:
RecName: Full=UDP-4-amino-4-deoxy-L-arabinose formyltransferase;
EC 2.1.2.13;
AltName: Full=ArnAFT;
AltName: Full=UDP-L-Ara4N formyltransferase;
Includes:
RecName: Full=UDP-glucuronic acid oxidase, UDP-4-keto-hexauronic acid decarboxylating;
EC 1.1.1.305;
AltName: Full=ArnADH;
AltName: Full=UDP-GlcUA decarboxylase;
AltName: Full=UDP-glucuronic acid dehydrogenase;
Gene name
arnA

Comments [?]

Function Bifunctional enzyme that catalyzes the oxidative decarboxylation of UDP-glucuronic acid (UDP-GlcUA) to UDP-4-keto-arabinose (UDP-Ara4O) and the addition of a formyl group to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N) to form UDP-L-4-formamido-arabinose (UDP-L-Ara4FN). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides.
Catalytic activity RHEA:24702: NAD(+) + UDP-alpha-D-glucuronate = CO2 + NADH + UDP-beta-L-threo-pentopyranos-4-ulose
EC 1.1.1.305
RHEA:24706: (6S)-10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinose = (6S)-5,6,7,8-tetrahydrofolate + H(+) + UDP-4-deoxy-4-formamido-beta-L-arabinose
EC 2.1.2.13
Pathway Nucleotide-sugar biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate: step 1/3.
Nucleotide-sugar biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate: step 3/3.
Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Subunit Homohexamer, formed by a dimer of trimers.
Similarity In the N-terminal section; belongs to the Fmt family. UDP-L-Ara4N formyltransferase subfamily.
In the C-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily.

Keywords [?]

Antibiotic resistance
Lipid A biosynthesis
Lipid biosynthesis
Lipid metabolism
Lipopolysaccharide biosynthesis
Multifunctional enzyme
NAD
Oxidoreductase
Transferase

Gene Ontology [?]

GO:0016616; Molecular function: oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor.
GO:0016742; Molecular function: hydroxymethyl-, formyl- and related transferase activity.
GO:0046493; Biological process: lipid A metabolic process.

Cross-references [?]

Pfam PF02911; Formyl_trans_C; 1;
PF00551; Formyl_trans_N; 1;
PF01370; Epimerase; 1;

Features [?]

From: ARNA_ECOLI (P77398)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     368     369       NAD binding     D-[IV]  
REGION     Nter     304       Formyltransferase ArnAFT        
REGION (Optional)     86     88       10-formyltetrahydrofolate binding     H-x-I  
REGION     136     140       10-formyltetrahydrofolate binding     [VIT]-x(3)-D  
REGION     314     Cter       Dehydrogenase ArnADH        
REGION     432     433       UDP-glucuronate binding     T-S  
REGION     526     535       UDP-glucuronate binding     [KRQ]-x(6)-Q-x-R  
ACT_SITE     104     104       Proton donor; for formyltransferase activity     H  
ACT_SITE     434     434       Proton acceptor; for decarboxylase activity     E  
ACT_SITE     619     619       Proton donor; for decarboxylase activity     R  
BINDING     114     114       10-formyltetrahydrofolate     R  
BINDING     347     347       NAD     D  
BINDING     393     393       UDP-glucuronate; via carbonyl oxygen     A  
BINDING     398     398       UDP-glucuronate     Y  
BINDING     460     460       UDP-glucuronate     R  
BINDING     492     492       UDP-glucuronate     N  
BINDING     613     613       UDP-glucuronate     Y  
SITE     102     102       Transition state stabilizer     N  
SITE     140     140       Raises pKa of active site His     D  

Additional information [?]

Size range 654-687 amino acids
Related rules MF_00182 (FMT)
Fusion None


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