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Annotation rule MF_01166 |
Accession | MF_01166 |
Dates | 11-NOV-2005 (Created) 19-NOV-2019 (Last updated, Version 28) |
Name | ArnA |
Scope | Bacteria; Gammaproteobacteria |
Templates | P77398 (ARNA_ECOLI); O52325 (ARNA_SALTY): [Recover all] |
Triggered by |
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Protein name |
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Gene name |
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Function | Bifunctional enzyme that catalyzes the oxidative decarboxylation of UDP-glucuronic acid (UDP-GlcUA) to UDP-4-keto-arabinose (UDP-Ara4O) and the addition of a formyl group to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N) to form UDP-L-4-formamido-arabinose (UDP-L-Ara4FN). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides. |
Catalytic activity | RHEA:24702: NAD(+) + UDP-alpha-D-glucuronate = CO2 + NADH + UDP-beta-L-threo-pentopyranos-4-ulose
EC 1.1.1.305 |
RHEA:24706: (6S)-10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinose = (6S)-5,6,7,8-tetrahydrofolate + H(+) + UDP-4-deoxy-4-formamido-beta-L-arabinose
EC 2.1.2.13 |
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Pathway | Nucleotide-sugar biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate: step 1/3. |
Nucleotide-sugar biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate: step 3/3. | |
Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. | |
Subunit | Homohexamer, formed by a dimer of trimers. |
Similarity | In the N-terminal section; belongs to the Fmt family. UDP-L-Ara4N formyltransferase subfamily. |
In the C-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily. |
From: ARNA_ECOLI (P77398) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
NP_BIND | 368 | 369 | NAD binding | D-[IV] | ||||||||
REGION | Nter | 304 | Formyltransferase ArnAFT | |||||||||
REGION (Optional) | 86 | 88 | 10-formyltetrahydrofolate binding | H-x-I | ||||||||
REGION | 136 | 140 | 10-formyltetrahydrofolate binding | [VIT]-x(3)-D | ||||||||
REGION | 314 | Cter | Dehydrogenase ArnADH | |||||||||
REGION | 432 | 433 | UDP-glucuronate binding | T-S | ||||||||
REGION | 526 | 535 | UDP-glucuronate binding | [KRQ]-x(6)-Q-x-R | ||||||||
ACT_SITE | 104 | 104 | Proton donor; for formyltransferase activity | H | ||||||||
ACT_SITE | 434 | 434 | Proton acceptor; for decarboxylase activity | E | ||||||||
ACT_SITE | 619 | 619 | Proton donor; for decarboxylase activity | R | ||||||||
BINDING | 114 | 114 | 10-formyltetrahydrofolate | R | ||||||||
BINDING | 347 | 347 | NAD | D | ||||||||
BINDING | 393 | 393 | UDP-glucuronate; via carbonyl oxygen | A | ||||||||
BINDING | 398 | 398 | UDP-glucuronate | Y | ||||||||
BINDING | 460 | 460 | UDP-glucuronate | R | ||||||||
BINDING | 492 | 492 | UDP-glucuronate | N | ||||||||
BINDING | 613 | 613 | UDP-glucuronate | Y | ||||||||
SITE | 102 | 102 | Transition state stabilizer | N | ||||||||
SITE | 140 | 140 | Raises pKa of active site His | D |
Size range | 654-687 amino acids |
Related rules | MF_00182 (FMT) |
Fusion | None |