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Annotation rule MF_01168 |
Accession | MF_01168 |
Dates | 8-DEC-2005 (Created) 19-NOV-2019 (Last updated, Version 13) |
Name | AGOG |
Scope | Archaea |
Template | Q8ZVK6 (AGOG_PYRAE) |
Triggered by |
Identifier |
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Protein name |
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Function | DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates. |
Catalytic activity | Reaction=The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.; EC=4.2.99.18; |
Domain | Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif). |
Similarity | Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family. |
Pfam | PF09171; DUF1886; 1; |
PIRSF | PIRSF008955; AGOG; 1; |
From: AGOG_PYRAE (Q8ZVK6) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 140 | 140 | Schiff-base intermediate with DNA | K | ||||||||
ACT_SITE | 172 | 172 | D | |||||||||
BINDING | 31 | 31 | 8-oxoguanine | Q | ||||||||
BINDING | 58 | 58 | 8-oxoguanine; via carbonyl oxygen | S | ||||||||
BINDING | 69 | 69 | 8-oxoguanine | W | ||||||||
BINDING | 144 | 144 | 8-oxoguanine | F | ||||||||
BINDING | 170 | 170 | 8-oxoguanine; via carbonyl oxygen | P | ||||||||
BINDING | 218 | 218 | 8-oxoguanine | D | ||||||||
BINDING | 222 | 222 | 8-oxoguanine | W | ||||||||
REGION | 125 | 184 | Helix-hairpin-helix |
Size range | 216-291 amino acids |
Related rules | None |
Fusion | None |