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Annotation rule MF_01168
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General rule information [?]

Accession MF_01168
Dates 8-DEC-2005 (Created)
8-JUN-2021 (Last updated, Version 13)
Name AGOG
Scope
Archaea
Template Q8ZVK6 (AGOG_PYRAE)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
AGOG
Protein name
RecName: Full=N-glycosylase/DNA lyase;
AltName: Full=8-oxoguanine DNA glycosylase;
EC 3.2.2.-;
AltName: Full=AGOG;
AltName: Full=DNA-(apurinic or apyrimidinic site) lyase;
Short=AP lyase;
EC 4.2.99.18;

Comments [?]

Function DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.
Catalytic activity RHEA:66592: (DNA)-(deoxyribonucleoside 5'-phosphate)-(deoxyribose 5'-phosphate)-(deoxyribonucleoside 5'-phosphate) = (DNA)-3'-(deoxyribonucleoside 5'-phosphate)-(2,3-dehydro-2,3-deoxyribose 5'-phosphate) + H(+) + phospho-5'-(DNA)
EC 4.2.99.18
Domain Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).
Similarity Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.

Keywords [?]

DNA damage
DNA excision
DNA repair
Hydrolase
Lyase

Gene Ontology [?]

GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0000702; Molecular function: oxidized base lesion DNA N-glycosylase activity.
GO:0006284; Biological process: base-excision repair.
GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam PF09171; DUF1886; 1;
PIRSF PIRSF008955; AGOG; 1;

Features [?]

From: AGOG_PYRAE (Q8ZVK6)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     140     140       Schiff-base intermediate with DNA     K  
ACT_SITE     172     172             D  
BINDING     31     31       8-oxoguanine     Q  
BINDING     58     58       8-oxoguanine; via carbonyl oxygen     S  
BINDING     69     69       8-oxoguanine     W  
BINDING     144     144       8-oxoguanine     F  
BINDING     170     170       8-oxoguanine; via carbonyl oxygen     P  
BINDING     218     218       8-oxoguanine     D  
BINDING     222     222       8-oxoguanine     W  
REGION     125     184       Helix-hairpin-helix        

Additional information [?]

Size range 216-291 amino acids
Related rules None
Fusion None


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