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Annotation rule MF_01168
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General rule information [?]

Accession MF_01168
Dates 8-DEC-2005 (Created)
19-NOV-2019 (Last updated, Version 13)
Template Q8ZVK6 (AGOG_PYRAE)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=N-glycosylase/DNA lyase;
AltName: Full=8-oxoguanine DNA glycosylase;
EC 3.2.2.-;
AltName: Full=AGOG;
AltName: Full=DNA-(apurinic or apyrimidinic site) lyase;
Short=AP lyase;

Comments [?]

Function DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.
Catalytic activity Reaction=The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.; EC=;
Domain Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).
Similarity Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.

Keywords [?]

Gene Ontology [?]

GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0000702; Molecular function: oxidized base lesion DNA N-glycosylase activity.
GO:0006284; Biological process: base-excision repair.
GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam PF09171; DUF1886; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     140     140       Schiff-base intermediate with DNA     K  
ACT_SITE     172     172             D  
BINDING     31     31       8-oxoguanine     Q  
BINDING     58     58       8-oxoguanine; via carbonyl oxygen     S  
BINDING     69     69       8-oxoguanine     W  
BINDING     144     144       8-oxoguanine     F  
BINDING     170     170       8-oxoguanine; via carbonyl oxygen     P  
BINDING     218     218       8-oxoguanine     D  
BINDING     222     222       8-oxoguanine     W  
REGION     125     184       Helix-hairpin-helix        

Additional information [?]

Size range 216-291 amino acids
Related rules None
Fusion None