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HAMAP rule MF_01168

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General rule information [?]

Accession MF_01168
Dates 8-DEC-2005 (Created)
19-NOV-2022 (Last updated, Version 17)
Name AGOG
Scope(s) Archaea
Template(s) Q8ZVK6 (AGOG_PYRAE); [ Recover all ]
Triggered by HAMAP; MF_01168 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier AGOG
Protein name RecName: Full=N-glycosylase/DNA lyase;
AltName: Full=8-oxoguanine DNA glycosylase;
                 EC=3.2.2.-;
AltName: Full=AGOG;
AltName: Full=DNA-(apurinic or apyrimidinic site) lyase;
                 Short=AP lyase;
                 EC=4.2.99.18;

Comments [?]

FUNCTIONDNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double- stranded DNA substrates.
CATALYTIC ACTIVITY Reaction=2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho- 2'-deoxyribonucleoside-DNA + H(+); Xref=Rhea:RHEA:66592, Rhea:RHEA- COMP:13180, Rhea:RHEA-COMP:16897, Rhea:RHEA-COMP:17067, ChEBI:CHEBI:15378, ChEBI:CHEBI:136412, ChEBI:CHEBI:157695, ChEBI:CHEBI:167181; EC=4.2.99.18;
DOMAINContains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix- hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).
SIMILARITYBelongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.

Keywords [?]


Gene Ontology [?]

GO:0003906; Molecular function:DNA-(apurinic or apyrimidinic site) endonuclease activity
GO:0000702; Molecular function:oxidized base lesion DNA N-glycosylase activity
GO:0006284; Biological process:base-excision repair
GO:0006281; Biological process:DNA repair

Cross-references [?]

Pfam PF09171; DUF1886; 1;
PIRSF PIRSF008955; AGOG; 1;

Features [?]

From: AGOG_PYRAE (Q8ZVK6)
Key From To Description Tag Condition FTGroup
ACT_SITE 140 140 /note="Schiff-base intermediate with DNA" K
ACT_SITE 172 172 D
BINDING 31 31 /ligand="8-oxoguanine"
/ligand_id="ChEBI:CHEBI:52617"
Q
BINDING 58 58 /ligand="8-oxoguanine"
/ligand_id="ChEBI:CHEBI:52617"
S
BINDING 69 69 /ligand="8-oxoguanine"
/ligand_id="ChEBI:CHEBI:52617"
W
BINDING 144 144 /ligand="8-oxoguanine"
/ligand_id="ChEBI:CHEBI:52617"
F
BINDING 170 170 /ligand="8-oxoguanine"
/ligand_id="ChEBI:CHEBI:52617"
P
BINDING 218 218 /ligand="8-oxoguanine"
/ligand_id="ChEBI:CHEBI:52617"
D
BINDING 222 222 /ligand="8-oxoguanine"
/ligand_id="ChEBI:CHEBI:52617"
W
REGION 125 184 /note="Helix-hairpin-helix"

Additional information [?]

Size range 216-291 amino acids
Related rules None
Fusion Nter: None Cter: None



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