HAMAP rule MF_01168
General rule information
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Accession | MF_01168 |
Dates | 8-DEC-2005 (Created) 19-NOV-2022 (Last updated, Version 17) |
Name | AGOG |
Scope | Archaea |
Template | Q8ZVK6 (AGOG_PYRAE) |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Comments
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Function | DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates. |
Catalytic activity | RHEA:66592: 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)
EC 4.2.99.18 |
Domain | Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif). |
Similarity | Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family. |
Keywords
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Gene Ontology
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GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0000702; Molecular function: oxidized base lesion DNA N-glycosylase activity.
GO:0006284; Biological process: base-excision repair.
GO:0006281; Biological process: DNA repair.
GO:0000702; Molecular function: oxidized base lesion DNA N-glycosylase activity.
GO:0006284; Biological process: base-excision repair.
GO:0006281; Biological process: DNA repair.
Cross-references
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Pfam | PF09171; DUF1886; 1; |
PIRSF | PIRSF008955; AGOG; 1; |
Features
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From: AGOG_PYRAE (Q8ZVK6) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 140 | 140 | Schiff-base intermediate with DNA | K | ||||||||
ACT_SITE | 172 | 172 | D | |||||||||
BINDING | 31 | 31 | /ligand="8-oxoguanine" /ligand_id="ChEBI:CHEBI:52617 | Q | ||||||||
BINDING | 58 | 58 | /ligand="8-oxoguanine" /ligand_id="ChEBI:CHEBI:52617 | S | ||||||||
BINDING | 69 | 69 | /ligand="8-oxoguanine" /ligand_id="ChEBI:CHEBI:52617 | W | ||||||||
BINDING | 144 | 144 | /ligand="8-oxoguanine" /ligand_id="ChEBI:CHEBI:52617 | F | ||||||||
BINDING | 170 | 170 | /ligand="8-oxoguanine" /ligand_id="ChEBI:CHEBI:52617 | P | ||||||||
BINDING | 218 | 218 | /ligand="8-oxoguanine" /ligand_id="ChEBI:CHEBI:52617 | D | ||||||||
BINDING | 222 | 222 | /ligand="8-oxoguanine" /ligand_id="ChEBI:CHEBI:52617 | W | ||||||||
REGION | 125 | 184 | Helix-hairpin-helix |
Additional information
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Size range | 216-291 amino acids |
Related rules | None |
Fusion | None |