HAMAP rule MF_01168

General rule information [?]

Accession	MF_01168
Dates	8-DEC-2005 (Created) 19-NOV-2022 (Last updated, Version 17)

Name

AGOG

Scope

Archaea

Template

Q8ZVK6 (AGOG_PYRAE)

Triggered by

HAMAP; MF_01168 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

AGOG

Protein name

RecName:		Full=N-glycosylase/DNA lyase;
AltName:		Full=8-oxoguanine DNA glycosylase;
		EC 3.2.2.-;
AltName:		Full=AGOG;
AltName:		Full=DNA-(apurinic or apyrimidinic site) lyase;
		Short=AP lyase;
		EC 4.2.99.18;

Comments [?]

Function	DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.
Catalytic activity	RHEA:66592: 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+) EC 4.2.99.18
Domain	Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).
Similarity	Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.

Keywords [?]

DNA damage
DNA excision
DNA repair
Hydrolase
Lyase

Gene Ontology [?]

GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0000702; Molecular function: oxidized base lesion DNA N-glycosylase activity.
GO:0006284; Biological process: base-excision repair.
GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam	PF09171; DUF1886; 1;
PIRSF	PIRSF008955; AGOG; 1;

Features [?]

From: AGOG_PYRAE (Q8ZVK6)

Key From To Description Tag Condition FTGroup

ACT_SITE 140 140 Schiff-base intermediate with DNA K

ACT_SITE 172 172 D

BINDING 31 31 /ligand="8-oxoguanine" /ligand_id="ChEBI:CHEBI:52617 Q

BINDING 58 58 /ligand="8-oxoguanine" /ligand_id="ChEBI:CHEBI:52617 S

BINDING 69 69 /ligand="8-oxoguanine" /ligand_id="ChEBI:CHEBI:52617 W

BINDING 144 144 /ligand="8-oxoguanine" /ligand_id="ChEBI:CHEBI:52617 F

BINDING 170 170 /ligand="8-oxoguanine" /ligand_id="ChEBI:CHEBI:52617 P

BINDING 218 218 /ligand="8-oxoguanine" /ligand_id="ChEBI:CHEBI:52617 D

BINDING 222 222 /ligand="8-oxoguanine" /ligand_id="ChEBI:CHEBI:52617 W

REGION 125 184 Helix-hairpin-helix

Additional information [?]

Size range	216-291 amino acids
Related rules	None
Fusion	None

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