HAMAP rule MF_01168

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_01168
Accession	MF_01168
Dates	08-DEC-2005 (Created) 30-JAN-2024 (Last updated, Version 19)
Name	AGOG
Scope(s)	Archaea
Template(s)	Q8ZVK6; [ Recover all ]
Triggered by	HAMAP; MF_01168 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	AGOG
Protein name	RecName: Full=N-glycosylase/DNA lyase; AltName: Full=8-oxoguanine DNA glycosylase; EC=3.2.2.-; AltName: Full=AGOG; AltName: Full=DNA-(apurinic or apyrimidinic site) lyase; Short=AP lyase; EC=4.2.99.18;

Comments [?]

FUNCTION	DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double- stranded DNA substrates.
CATALYTIC ACTIVITY	Reaction=2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'- deoxyribonucleoside-DNA + H(+); Xref=Rhea:RHEA:66592, Rhea:RHEA- COMP:13180, Rhea:RHEA-COMP:16897, Rhea:RHEA-COMP:17067, ChEBI:CHEBI:15378, ChEBI:CHEBI:136412, ChEBI:CHEBI:157695, ChEBI:CHEBI:167181; EC=4.2.99.18;
DOMAIN	Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix- hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).
SIMILARITY	Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.

Keywords [?]

Gene Ontology [?]

GO:0003906; Molecular function:DNA-(apurinic or apyrimidinic site) endonuclease activity

GO:0000702; Molecular function:oxidized base lesion DNA N-glycosylase activity

GO:0006284; Biological process:base-excision repair

GO:0006281; Biological process:DNA repair

Cross-references [?]

Pfam	PF09171; DUF1886; 1;
PIRSF	PIRSF008955; AGOG; 1;

Features [?]

From: AGOG_PYRAE (Q8ZVK6)

Key

From

Description

Tag

Condition

FTGroup

ACT_SITE

140

/note="Schiff-base intermediate with DNA"

ACT_SITE

172

BINDING