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HAMAP rule MF_01210

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General rule information [?]

Accession MF_01210
Dates 19-JUL-2002 (Created)
1-JUN-2023 (Last updated, Version 50)
Name CPSase_L_chain
Scope(s) Bacteria
Archaea
Template(s) P00968 (CARB_ECOLI); O50302 (CARB_GEOSE); Q9ZB63 (CARY_GEOSE); O32771 (CARB_LACLM); P77886 (CARB_LACPL); Q9RLS9 (CARY_LACPL); [ Recover all ]
Triggered by
case c? <OC:Bacteria>
HAMAP; MF_01210_B (Get profile general information and statistics)
end case
case c? <OC:Archaea>
HAMAP; MF_01210_A (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier CARB
Protein name RecName: Full=Carbamoyl-phosphate synthase large chain;
                 EC=6.3.5.5;
AltName: Full=Carbamoyl-phosphate synthetase ammonia chain;
Gene name Name=carB;

Comments [?]

CATALYTIC ACTIVITY Reaction=2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate; Xref=Rhea:RHEA:18633, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58228, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.5.5;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 4 Mg(2+) or Mn(2+) ions per subunit.
PATHWAYAmino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
PATHWAYPyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.
SUBUNITComposed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.
SIMILARITYBelongs to the CarB family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004088; Molecular function:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
GO:0006526; Biological process:arginine biosynthetic process
GO:0006221; Biological process:pyrimidine nucleotide biosynthetic process

Cross-references [?]

Pfam PF00289; CPSase_L_chain; 1;
Pfam PF02142; MGS; 1;
Pfam PF02786; CPSase_L_D2; 1;
Pfam PF02787; CPSase_L_D3; 1;
PRINTS PR00098; CPSASE; 1;
PROSITE PS00866; CPSASE_1; 1;
PROSITE PS00867; CPSASE_2; 1;
PROSITE PS50975; ATP_GRASP; 1-2;
PROSITE PS51855; MGS; 1;
NCBIfam TIGR01369; CPSaseII_lrg; 1;

Features [?]

From: CARB_ECOLI (P00968)
Key From To Description Tag Condition FTGroup
case <OC:Bacteria>
REGION Nter 403 /note="Carboxyphosphate synthetic domain"
REGION 404 553 /note="Oligomerization domain"
REGION 554 936 /note="Carbamoyl phosphate synthetic domain"
REGION 937 Cter /note="Allosteric domain"
BINDING 285 285 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"
Q
BINDING 299 299 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"
E
BINDING 299 299 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"
E
BINDING 301 301 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"
N
BINDING 829 829 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="3"
Q
BINDING 841 841 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="3"
E
BINDING 841 841 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="4"
E
BINDING 843 843 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="4"
N
end case
From: CARB_SULTO (Q970U7)
Key From To Description Tag Condition FTGroup
case <OC:Archaea>
REGION Nter 399 /note="Carboxyphosphate synthetic domain"
REGION 400 548 /note="Oligomerization domain"
REGION 549 930 /note="Carbamoyl phosphate synthetic domain"
REGION 931 Cter /note="Allosteric domain"
BINDING 282 282 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"
Q
BINDING 296 296 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"
E
BINDING 296 296 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"
E
BINDING 298 298 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"
N
BINDING 823 823 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="3"
Q
BINDING 835 835 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="3"
E
BINDING 835 835 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="4"
E
BINDING 837 837 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="4"
N
end case

Additional information [?]

Size range 1024-1076 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Bacillaceae, Geobacillus and lactobacillaceae seem to posess two members of this family: one specific for arginine biosynthesis (called CARY_ in ID, and CARB in GN) and the other specific for pyrimidine synthesis (called CARB_ in ID, and PYRAB in GN). Sequence of carB is split into two genes in AQUAE, METJA and METKA. Weird insertion in NOSS1, THEMA and ZYMMO (sequences not shown in alignment).



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