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| Annotation rule MF_01210 |
General rule information
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| Accession | MF_01210 |
| Dates | 19-JUL-2002 (Created) 19-NOV-2019 (Last updated, Version 44) |
| Name | CPSase_L_chain |
| Scope | Bacteria
Archaea |
| Templates | P00968 (CARB_ECOLI); O50302 (CARB_GEOSE); Q9ZB63 (CARY_GEOSE); O32771 (CARB_LACLM); P77886 (CARB_LACPL); Q9RLS9 (CARY_LACPL): [Recover all] |
case <OC:Bacteria>
| Triggered by |
end case
case <OC:Archaea>
| Triggered by |
end case
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Catalytic activity | RHEA:18633: 2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate
EC 6.3.5.5 |
| Pathway | Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. |
| Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. | |
| Subunit | Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. |
| Similarity | Belongs to the CarB family. |
Keywords
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Amino-acid biosynthesis
Arginine biosynthesis
Pyrimidine biosynthesis
Ligase
Repeat
ATP-binding
Nucleotide-binding
Arginine biosynthesis
Pyrimidine biosynthesis
Ligase
Repeat
ATP-binding
Nucleotide-binding
Gene Ontology
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GO:0005524; Molecular function: ATP binding.
GO:0004088; Molecular function: carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity.
GO:0006526; Biological process: arginine biosynthetic process.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.
GO:0004088; Molecular function: carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity.
GO:0006526; Biological process: arginine biosynthetic process.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.
Cross-references
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| Pfam | PF00289; CPSase_L_chain; 1; |
| PF02142; MGS; 1; | |
| PF02786; CPSase_L_D2; 1; | |
| PF02787; CPSase_L_D3; 1; | |
| PRINTS | PR00098; CPSASE; 1; |
| PROSITE | PS00866; CPSASE_1; 1; |
| PS00867; CPSASE_2; 1; | |
| PS50975; ATP_GRASP; 1-2; trigger=PRU00409; | |
| PS51855; MGS; 1; trigger=PRU01202; | |
| TIGRFAMs | TIGR01369; CPSaseII_lrg; 1; |
Features
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case <OC:Bacteria>
| From: CARB_ECOLI (P00968) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | Nter | 403 | Carboxyphosphate synthetic domain | |||||||||
| REGION | 404 | 553 | Oligomerization domain | |||||||||
| REGION | 554 | 936 | Carbamoyl phosphate synthetic domain | |||||||||
| REGION | 937 | Cter | Allosteric domain | |||||||||
end case
case <OC:Archaea>
| From: CARB_SULTO (Q970U7) | ||||||||||||
| REGION | Nter | 399 | Carboxyphosphate synthetic domain | |||||||||
| REGION | 400 | 548 | Oligomerization domain | |||||||||
| REGION | 549 | 930 | Carbamoyl phosphate synthetic domain | |||||||||
| REGION | 931 | Cter | Allosteric domain | |||||||||
end case
Additional information
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case <OC:Bacteria>
| Size range | 1020-1167 amino acids |
end case
case <OC:Archaea>
| Size range | 1024-1076 amino acids |
end case
| Related rules | None |
| Fusion | None |
| Comments | Bacillaceae, Geobacillus and lactobacillaceae seem to posess two members of this family: one specific for arginine biosynthesis (called CARY_ in ID, and CARB in GN) and the other specific for pyrimidine synthesis (called CARB_ in ID, and PYRAB in GN). Sequence of carB is split into two genes in AQUAE, METJA and METKA. Weird insertion in NOSS1, THEMA and ZYMMO (sequences not shown in alignment). |