HAMAP rule MF_01210
General rule information
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| Accession | MF_01210 |
| Dates | 19-JUL-2002 (Created)
1-JUN-2023 (Last updated, Version 50) |
| Name | CPSase_L_chain |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P00968 (CARB_ECOLI); O50302 (CARB_GEOSE); Q9ZB63 (CARY_GEOSE); O32771 (CARB_LACLM); P77886 (CARB_LACPL); Q9RLS9 (CARY_LACPL); [ Recover all ] |
| Triggered by |
case c? <OC:Bacteria>
HAMAP; MF_01210_B (Get profile general information and statistics) end case
case c? <OC:Archaea>
HAMAP; MF_01210_A (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| Identifier | CARB |
| Protein name | RecName: Full=Carbamoyl-phosphate synthase large chain; EC=6.3.5.5; AltName: Full=Carbamoyl-phosphate synthetase ammonia chain; |
| Gene name | Name=carB; |
Comments
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| CATALYTIC ACTIVITY | Reaction=2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate; Xref=Rhea:RHEA:18633, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58228, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.5.5; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 4 Mg(2+) or Mn(2+) ions per subunit. |
| PATHWAY | Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. |
| PATHWAY | Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. |
| SUBUNIT | Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. |
| SIMILARITY | Belongs to the CarB family. |
Keywords
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| Amino-acid biosynthesis |
| Arginine biosynthesis |
| Pyrimidine biosynthesis |
| Ligase |
| Repeat |
| ATP-binding |
| Nucleotide-binding |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0004088; Molecular function:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity |
| GO:0006526; Biological process:arginine biosynthetic process |
| GO:0006221; Biological process:pyrimidine nucleotide biosynthetic process |
Cross-references
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| Pfam | PF00289; CPSase_L_chain; 1; |
| Pfam | PF02142; MGS; 1; |
| Pfam | PF02786; CPSase_L_D2; 1; |
| Pfam | PF02787; CPSase_L_D3; 1; |
| PRINTS | PR00098; CPSASE; 1; |
| PROSITE | PS00866; CPSASE_1; 1; |
| PROSITE | PS00867; CPSASE_2; 1; |
| PROSITE | PS50975; ATP_GRASP; 1-2; |
| PROSITE | PS51855; MGS; 1; |
| NCBIfam | TIGR01369; CPSaseII_lrg; 1; |
Features
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| From: CARB_ECOLI (P00968) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Bacteria> | ||||||||||||
| REGION | Nter | 403 | /note="Carboxyphosphate synthetic domain" | |||||||||
| REGION | 404 | 553 | /note="Oligomerization domain" | |||||||||
| REGION | 554 | 936 | /note="Carbamoyl phosphate synthetic domain" | |||||||||
| REGION | 937 | Cter | /note="Allosteric domain" | |||||||||
| BINDING | 285 | 285 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
Q | ||||||||
| BINDING | 299 | 299 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
E | ||||||||
| BINDING | 299 | 299 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
E | ||||||||
| BINDING | 301 | 301 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
N | ||||||||
| BINDING | 829 | 829 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="3" |
Q | ||||||||
| BINDING | 841 | 841 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="3" |
E | ||||||||
| BINDING | 841 | 841 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="4" |
E | ||||||||
| BINDING | 843 | 843 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="4" |
N | ||||||||
| end case | ||||||||||||
| From: CARB_SULTO (Q970U7) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Archaea> | ||||||||||||
| REGION | Nter | 399 | /note="Carboxyphosphate synthetic domain" | |||||||||
| REGION | 400 | 548 | /note="Oligomerization domain" | |||||||||
| REGION | 549 | 930 | /note="Carbamoyl phosphate synthetic domain" | |||||||||
| REGION | 931 | Cter | /note="Allosteric domain" | |||||||||
| BINDING | 282 | 282 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
Q | ||||||||
| BINDING | 296 | 296 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
E | ||||||||
| BINDING | 296 | 296 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
E | ||||||||
| BINDING | 298 | 298 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
N | ||||||||
| BINDING | 823 | 823 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="3" |
Q | ||||||||
| BINDING | 835 | 835 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="3" |
E | ||||||||
| BINDING | 835 | 835 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="4" |
E | ||||||||
| BINDING | 837 | 837 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="4" |
N | ||||||||
| end case | ||||||||||||
Additional information
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| Size range | 1024-1076 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Bacillaceae, Geobacillus and lactobacillaceae seem to posess two members of this family: one specific for arginine biosynthesis (called CARY_ in ID, and CARB in GN) and the other specific for pyrimidine synthesis (called CARB_ in ID, and PYRAB in GN). Sequence of carB is split into two genes in AQUAE, METJA and METKA. Weird insertion in NOSS1, THEMA and ZYMMO (sequences not shown in alignment). |