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Annotation rule MF_01210
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General rule information [?]

Accession MF_01210
Dates 19-JUL-2002 (Created)
19-NOV-2019 (Last updated, Version 44)
Name CPSase_L_chain
Templates P00968 (CARB_ECOLI); O50302 (CARB_GEOSE); Q9ZB63 (CARY_GEOSE); O32771 (CARB_LACLM); P77886 (CARB_LACPL); Q9RLS9 (CARY_LACPL): [Recover all]
case <OC:Bacteria>
end case

case <OC:Archaea>
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Carbamoyl-phosphate synthase large chain;
AltName: Full=Carbamoyl-phosphate synthetase ammonia chain;
Gene name

Comments [?]

Catalytic activity RHEA:18633: 2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate
Pathway Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.
Subunit Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.
Similarity Belongs to the CarB family.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004088; Molecular function: carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity.
GO:0006526; Biological process: arginine biosynthetic process.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.

Cross-references [?]

Pfam PF00289; CPSase_L_chain; 1;
PF02142; MGS; 1;
PF02786; CPSase_L_D2; 1;
PF02787; CPSase_L_D3; 1;
PS00867; CPSASE_2; 1;
PS50975; ATP_GRASP; 1-2; trigger=PRU00409;
PS51855; MGS; 1; trigger=PRU01202;
TIGRFAMs TIGR01369; CPSaseII_lrg; 1;

Features [?]

case <OC:Bacteria>
From: CARB_ECOLI (P00968)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     Nter     403       Carboxyphosphate synthetic domain        
REGION     404     553       Oligomerization domain        
REGION     554     936       Carbamoyl phosphate synthetic domain        
REGION     937     Cter       Allosteric domain        
end case
case <OC:Archaea>
From: CARB_SULTO (Q970U7)
REGION     Nter     399       Carboxyphosphate synthetic domain        
REGION     400     548       Oligomerization domain        
REGION     549     930       Carbamoyl phosphate synthetic domain        
REGION     931     Cter       Allosteric domain        
end case

Additional information [?]

case <OC:Bacteria>
Size range 1020-1167 amino acids
end case
case <OC:Archaea>
Size range 1024-1076 amino acids
end case
Related rules None
Fusion None
Comments Bacillaceae, Geobacillus and lactobacillaceae seem to posess two members of this family: one specific for arginine biosynthesis (called CARY_ in ID, and CARB in GN) and the other specific for pyrimidine synthesis (called CARB_ in ID, and PYRAB in GN). Sequence of carB is split into two genes in AQUAE, METJA and METKA. Weird insertion in NOSS1, THEMA and ZYMMO (sequences not shown in alignment).