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HAMAP rule MF_01216

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General rule information [?]

Accession MF_01216
Dates 24-JUN-2002 (Created)
28-NOV-2023 (Last updated, Version 42)
Name Azoreductase_type1
Scope(s) Bacteria
Template(s) P41407 (AZOR_ECOLI); Q9I5F3 (AZOR1_PSEAE); Q9I2E2 (AZOR2_PSEAE); Q9HZ17 (AZOR3_PSEAE); Q831B2 (AZOR_ENTFA); [ Recover all ]
Triggered by HAMAP; MF_01216 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier AZOR
Protein name RecName: Full=FMN dependent NADH:quinone oxidoreductase;
AltName: Full=Azo-dye reductase;
AltName: Full=FMN-dependent NADH-azo compound oxidoreductase;
AltName: Full=FMN-dependent NADH-azoreductase;
Gene name Name=azoR;

Comments [?]

FUNCTIONQuinone reductase that provides resistance to thiol-specific stress caused by electrophilic quinones.
FUNCTIONAlso exhibits azoreductase activity. Catalyzes the reductive cleavage of the azo bond in aromatic azo compounds to the corresponding amines.
CATALYTIC ACTIVITY Reaction=2 a quinone + H(+) + NADH = 2 a 1,4-benzosemiquinone + NAD(+); Xref=Rhea:RHEA:65952, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124, ChEBI:CHEBI:134225;
CATALYTIC ACTIVITY Reaction=anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD(+) = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 H(+) + 2 NADH; Xref=Rhea:RHEA:55872, ChEBI:CHEBI:15378, ChEBI:CHEBI:15783, ChEBI:CHEBI:16567, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:71579; EC=;
COFACTOR Name=FMN; Xref=ChEBI:CHEBI:58210; Note=Binds 1 FMN per subunit.;
SIMILARITYBelongs to the azoreductase type 1 family.

Keywords [?]

Gene Ontology [?]

GO:0009055; Molecular function:electron transfer activity
GO:0010181; Molecular function:FMN binding
GO:0016652; Molecular function:oxidoreductase activity, acting on NAD(P)H, NAD(P) as acceptor

Cross-references [?]

Pfam PF02525; Flavodoxin_2; 1;

Features [?]

From: AZOR1_PSEAE (Q9I5F3)
Key From To Description Tag Condition FTGroup
BINDING 16 18 /ligand="FMN"
BINDING 97 100 /ligand="FMN"
BINDING 145 148 /ligand="FMN"
BINDING 10 10 /ligand="FMN"

Additional information [?]

Size range 186-240 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Divergent STRCO and first copy in CLOAB and CLOPE; sequences not included in alignment and not taken into account in size range. In some species there are many duplicates.

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