HAMAP rule MF_01216
General rule information
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| Accession | MF_01216 |
| Dates | 24-JUN-2002 (Created)
3-SEP-2024 (Last updated, Version 43) |
| Name | Azoreductase_type1 |
| Scope(s) |
Bacteria |
| Template(s) | P41407 (AZOR_ECOLI); Q9I5F3 (AZOR1_PSEAE); Q9I2E2 (AZOR2_PSEAE); Q9HZ17 (AZOR3_PSEAE); Q831B2 (AZOR_ENTFA); [ Recover all ] |
| Triggered by |
HAMAP; MF_01216 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | AZOR |
| Protein name | RecName: Full=FMN dependent NADH:quinone oxidoreductase; EC=1.6.5.-; AltName: Full=Azo-dye reductase; AltName: Full=FMN-dependent NADH-azo compound oxidoreductase; AltName: Full=FMN-dependent NADH-azoreductase; EC=1.7.1.17; |
| Gene name | Name=azoR; |
Comments
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| FUNCTION | Quinone reductase that provides resistance to thiol-specific stress caused by electrophilic quinones. |
| FUNCTION | Also exhibits azoreductase activity. Catalyzes the reductive cleavage of the azo bond in aromatic azo compounds to the corresponding amines. |
| CATALYTIC ACTIVITY | Reaction=2 a quinone + NADH + H(+) = 2 a 1,4-benzosemiquinone + NAD(+); Xref=Rhea:RHEA:65952, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124, ChEBI:CHEBI:134225; |
| CATALYTIC ACTIVITY | Reaction=N,N-dimethyl-1,4-phenylenediamine + anthranilate + 2 NAD(+) = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H(+); Xref=Rhea:RHEA:55872, ChEBI:CHEBI:15378, ChEBI:CHEBI:15783, ChEBI:CHEBI:16567, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:71579; EC=1.7.1.17; |
| COFACTOR | Name=FMN; Xref=ChEBI:CHEBI:58210; Note=Binds 1 FMN per subunit.; |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the azoreductase type 1 family. |
Keywords
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Gene Ontology
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| GO:0009055; Molecular function:electron transfer activity |
| GO:0010181; Molecular function:FMN binding |
| GO:0016652; Molecular function:oxidoreductase activity, acting on NAD(P)H as acceptor |
Cross-references
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Features
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| From: AZOR1_PSEAE (Q9I5F3) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 16 | 18 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
S-x-S | ||||||||
| BINDING | 97 | 100 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
M-[YW]-N-[FL] | ||||||||
| BINDING | 145 | 148 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
[ST]-x-G-G | ||||||||
| BINDING | 10 | 10 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
S | ||||||||
Additional information
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| Size range | 186-240 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Divergent STRCO and first copy in CLOAB and CLOPE; sequences not included in alignment and not taken into account in size range. In some species there are many duplicates. |