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HAMAP rule MF_01216

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General rule information [?]

Accession MF_01216
Dates 24-JUN-2002 (Created)
17-JAN-2023 (Last updated, Version 41)
Name Azoreductase_type1
Templates P41407 (AZOR_ECOLI); Q9I5F3 (AZOR1_PSEAE); Q9I2E2 (AZOR2_PSEAE); Q9HZ17 (AZOR3_PSEAE); Q831B2 (AZOR_ENTFA): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=FMN dependent NADH:quinone oxidoreductase;
EC 1.6.5.-;
AltName: Full=Azo-dye reductase;
AltName: Full=FMN-dependent NADH-azo compound oxidoreductase;
AltName: Full=FMN-dependent NADH-azoreductase;
Gene name

Comments [?]

Function Quinone reductase that provides resistance to thiol-specific stress caused by electrophilic quinones.
Also exhibits azoreductase activity. Catalyzes the reductive cleavage of the azo bond in aromatic azo compounds to the corresponding amines.
Catalytic activity RHEA:65952: 2 a quinone + H(+) + NADH = 2 a 1,4-benzosemiquinone + NAD(+)
RHEA:55872: anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD(+) = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 H(+) + 2 NADH
Cofactor FMN
Note: Binds 1 FMN per subunit.
Subunit Homodimer.
Similarity Belongs to the azoreductase type 1 family.

Keywords [?]

Gene Ontology [?]

GO:0009055; Molecular function: electron transfer activity.
GO:0010181; Molecular function: FMN binding.
GO:0016652; Molecular function: oxidoreductase activity, acting on NAD(P)H, NAD(P) as acceptor.

Cross-references [?]

Pfam PF02525; Flavodoxin_2; 1;

Features [?]

From: AZOR1_PSEAE (Q9I5F3)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     16     18       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     S-x-S  
BINDING     97     100       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     M-[YW]-N-[FL]  
BINDING     145     148       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     [ST]-x-G-G  
BINDING     10     10       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     S  

Additional information [?]

Size range 186-240 amino acids
Related rules None
Fusion None
Comments Divergent STRCO and first copy in CLOAB and CLOPE; sequences not included in alignment and not taken into account in size range. In some species there are many duplicates.