HAMAP rule MF_01258
General rule information
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Accession | MF_01258 |
Dates | 21-APR-2005 (Created) 1-JUN-2023 (Last updated, Version 21) |
Name | F420_ligase_CofE |
Scope | Archaea; Archaeoglobi
Archaea; Halobacteria
Archaea; Methanobacteria
Archaea; Methanococci
Archaea; Methanomicrobia
Archaea; Methanopyri |
Templates | Q58178 (COFE_METJA); O28028 (COFE_ARCFU): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Catalyzes the GTP-dependent successive addition of two or more gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2) or polyglutamated F420 derivatives. |
Catalytic activity | RHEA:30555: GTP + L-glutamate + oxidized coenzyme F420-0 = GDP + H(+) + oxidized coenzyme F420-1 + phosphate
EC 6.3.2.31 |
RHEA:30523: GTP + L-glutamate + oxidized coenzyme F420-1 = GDP + H(+) + oxidized coenzyme F420-2 + phosphate
EC 6.3.2.34 |
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Cofactor | Mg(2+) Mn(2+) Note: Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese. |
K(+) Note: Monovalent cation. The ion could be potassium. |
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Pathway | Cofactor biosynthesis; coenzyme F420 biosynthesis. |
Subunit | Homodimer. |
Similarity | Belongs to the CofE family. |
Keywords
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Gene Ontology
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GO:0052618; Molecular function: coenzyme F420-0:L-glutamate ligase activity.
GO:0052619; Molecular function: coenzyme F420-1:gamma-L-glutamate ligase activity.
GO:0052645; Biological process: F420-0 metabolic process.
GO:0052619; Molecular function: coenzyme F420-1:gamma-L-glutamate ligase activity.
GO:0052645; Biological process: F420-0 metabolic process.
Cross-references
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Features
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From: COFE_ARCFU (O28028) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 11 | 14 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | [LIMV]-P-x-[IVEF] | ||||||||
BINDING | 40 | 41 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | [SEH]-T | ||||||||
BINDING | 206 | 213 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | |||||||||
BINDING | 109 | 109 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1 | D | ||||||||
BINDING | 150 | 150 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1 | D | ||||||||
BINDING | 151 | 151 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2 | [TS] | ||||||||
BINDING | 208 | 208 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2 | [EQ] | ||||||||
BINDING | 45 | 45 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | K | ||||||||
BINDING | 112 | 112 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | N |
Additional information
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Size range | 248-257 amino acids |
Related rules | MF_01259 (FBIB supersedes the current rule) |
Fusion | None |
Comments | CofE homologs are found in the genomes of all organisms currently known to produce coenzyme F420. These include not only the methanogenic archaea but also nonmethanogenic archaea such as Halobacteria and Archaeoglobus, as well as some eubacteria such as Streptomyces, Mycobacterium, Nocardia, and Thermobifida (see MF_01259). The length of the polyglutamate tail varies in different organisms. According to PubMed:12867481 (Graupner et al., 2003) and PubMed:11479701 (Bair et al., 2001): Analyses of the F420s present in Methanococcus jannaschii have shown that these cells contain a series of gamma-glutamyl-linked F420s capped with a single, terminal alpha-linked L-glutamate. The predominant form of F420 was designated as alpha-F420-3 and represented 86% of the F420s in these cells. Analyses of Methanosarcina thermophila, Methanosarcina barkeri, Methanobacterium thermoautotrophicum, Archaeoglobus fulgidus, and Mycobacterium smegmatis showed that they contained only gamma-glutamyl-linked F420s, with the following predominant forms: Methanosarcina thermophila (gamma-F420-3, 19%; gamma-F420-4, 62%), Methanosarcina barkeri (gamma-F420-2, 31%; gamma-F420-4, 32%), Methanobacterium thermoautotrophicum (gamma-F420-2, 80%), Archaeoglobus fulgidus (gamma-F420-4, 89%), and Mycobacterium smegmatis (gamma-F420-5, 67%; gamma-F420-6, 31%). In mycobacteria, the length of the polyglutamate tail varies by up to nine residues, with predominant species containing four to seven glutamate molecules (Bair et al., 2001, PubMed:11479701; Bashiri et al., 2008, PubMed:18434308). |