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HAMAP rule MF_01258

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General rule information [?]

Accession MF_01258
Dates 21-APR-2005 (Created)
1-JUN-2023 (Last updated, Version 21)
Name F420_ligase_CofE
Scope(s) Archaea
Template(s) Q58178 (COFE_METJA); O28028 (COFE_ARCFU); [ Recover all ]
Triggered by HAMAP; MF_01258 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier COFE
Protein name RecName: Full=Coenzyme F420:L-glutamate ligase;
AltName: Full=Coenzyme F420-0:L-glutamate ligase;
AltName: Full=Coenzyme F420-1:gamma-L-glutamate ligase;
Gene name Name=cofE;

Comments [?]

FUNCTIONCatalyzes the GTP-dependent successive addition of two or more gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8- didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form coenzyme F420- 0-glutamyl-glutamate (F420-2) or polyglutamated F420 derivatives.
CATALYTIC ACTIVITY Reaction=GTP + L-glutamate + oxidized coenzyme F420-0 = GDP + H(+) + oxidized coenzyme F420-1 + phosphate; Xref=Rhea:RHEA:30555, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189, ChEBI:CHEBI:59907, ChEBI:CHEBI:59920; EC=;
CATALYTIC ACTIVITY Reaction=GTP + L-glutamate + oxidized coenzyme F420-1 = GDP + H(+) + oxidized coenzyme F420-2 + phosphate; Xref=Rhea:RHEA:30523, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:57922, ChEBI:CHEBI:58189, ChEBI:CHEBI:59920; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.;
COFACTOR Name=K(+); Xref=ChEBI:CHEBI:29103; Note=Monovalent cation. The ion could be potassium.;
PATHWAYCofactor biosynthesis; coenzyme F420 biosynthesis.
SIMILARITYBelongs to the CofE family.

Keywords [?]

Gene Ontology [?]

GO:0052618; Molecular function:coenzyme F420-0:L-glutamate ligase activity
GO:0052619; Molecular function:coenzyme F420-1:gamma-L-glutamate ligase activity
GO:0052645; Biological process:F420-0 metabolic process

Cross-references [?]

Pfam PF01996; F420_ligase; 1;
NCBIfam TIGR01916; F420_cofE; 1;

Features [?]

From: COFE_ARCFU (O28028)
Key From To Description Tag Condition FTGroup
BINDING 11 14 /ligand="GTP"
BINDING 40 41 /ligand="GTP"
BINDING 206 213 /ligand="GTP"
BINDING 109 109 /ligand="a divalent metal cation"
BINDING 150 150 /ligand="a divalent metal cation"
BINDING 151 151 /ligand="a divalent metal cation"
BINDING 208 208 /ligand="a divalent metal cation"
BINDING 45 45 /ligand="GTP"
BINDING 112 112 /ligand="GTP"

Additional information [?]

Size range 248-257 amino acids
Related rules MF_01259
Fusion Nter: None Cter: None
Comments CofE homologs are found in the genomes of all organisms currently known to produce coenzyme F420. These include not only the methanogenic archaea but also nonmethanogenic archaea such as Halobacteria and Archaeoglobus, as well as some eubacteria such as Streptomyces, Mycobacterium, Nocardia, and Thermobifida (see MF_01259). The length of the polyglutamate tail varies in different organisms. According to PubMed:12867481 (Graupner et al., 2003) and PubMed:11479701 (Bair et al., 2001): Analyses of the F420s present in Methanococcus jannaschii have shown that these cells contain a series of gamma-glutamyl-linked F420s capped with a single, terminal alpha-linked L-glutamate. The predominant form of F420 was designated as alpha-F420-3 and represented 86% of the F420s in these cells. Analyses of Methanosarcina thermophila, Methanosarcina barkeri, Methanobacterium thermoautotrophicum, Archaeoglobus fulgidus, and Mycobacterium smegmatis showed that they contained only gamma-glutamyl-linked F420s, with the following predominant forms: Methanosarcina thermophila (gamma-F420-3, 19%; gamma-F420-4, 62%), Methanosarcina barkeri (gamma-F420-2, 31%; gamma-F420-4, 32%), Methanobacterium thermoautotrophicum (gamma-F420-2, 80%), Archaeoglobus fulgidus (gamma-F420-4, 89%), and Mycobacterium smegmatis (gamma-F420-5, 67%; gamma-F420-6, 31%). In mycobacteria, the length of the polyglutamate tail varies by up to nine residues, with predominant species containing four to seven glutamate molecules (Bair et al., 2001, PubMed:11479701; Bashiri et al., 2008, PubMed:18434308).

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