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Annotation rule MF_01260
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General rule information [?]

Accession MF_01260
Dates 26-JUL-2005 (Created)
21-JAN-2020 (Last updated, Version 22)
Name Carboxylester
Scope
Bacteria; Proteobacteria
Templates P13001 (BIOH_ECOLI); Q9K197 (BIOH_NEIMB); Q8GHL1 (BIOH_SERMA): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
BIOH
Protein name
RecName: Full=Pimeloyl-[acyl-carrier protein] methyl ester esterase;
EC 3.1.1.85;
AltName: Full=Biotin synthesis protein BioH;
AltName: Full=Carboxylesterase BioH;
Gene name
bioH

Comments [?]

Function The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.
Catalytic activity RHEA:42700: 6-carboxyhexanoyl-[ACP] methyl ester + H2O = 6-carboxyhexanoyl-[ACP] + H(+) + methanol
EC 3.1.1.85
Pathway Cofactor biosynthesis; biotin biosynthesis.
Subunit Monomer.
Subcellular location Cytoplasm.
Similarity Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.

Keywords [?]

Cytoplasm
Biotin biosynthesis
Hydrolase
Serine esterase

Gene Ontology [?]

GO:0052689; Molecular function: carboxylic ester hydrolase activity.
GO:0009102; Biological process: biotin biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00561; Abhydrolase_1; 1;
PF00975; Thioesterase; 1;
PRINTS PR00111; ABHYDROLASE; 1;
PR00412; EPOXHYDRLASE; 1;
TIGRFAMs TIGR01738; BioH; 1;

Features [?]

From: BIOH_ECOLI (P13001)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     82     83       Substrate binding     S-[LM]  
REGION     143     147       Substrate binding     F-x-x-x-[QD]  
ACT_SITE     82     82       Nucleophile     S  
ACT_SITE     207     207             D  
ACT_SITE     235     235             H  
BINDING     22     22       Substrate; via amide nitrogen and carbonyl oxygen     W  
BINDING     235     235       Substrate     H  

Additional information [?]

Size range 239-312 amino acids
Related rules None
Fusion Nter: None; Cter: MF_00835 (BioC)
Comments Longer C-terminal in NEIMA; sequence not shown in alignment and not taken into account in size range


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