![]() |
|
Annotation rule MF_01260 |
Accession | MF_01260 |
Dates | 26-JUL-2005 (Created) 21-JAN-2020 (Last updated, Version 22) |
Name | Carboxylester |
Scope | Bacteria; Proteobacteria |
Templates | P13001 (BIOH_ECOLI); Q9K197 (BIOH_NEIMB); Q8GHL1 (BIOH_SERMA): [Recover all] |
Triggered by |
Identifier |
|
Protein name |
|
Gene name |
|
Function | The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters. |
Catalytic activity | RHEA:42700: 6-carboxyhexanoyl-[ACP] methyl ester + H2O = 6-carboxyhexanoyl-[ACP] + H(+) + methanol
EC 3.1.1.85 |
Pathway | Cofactor biosynthesis; biotin biosynthesis. |
Subunit | Monomer. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family. |
Pfam | PF00561; Abhydrolase_1; 1; |
PF00975; Thioesterase; 1; | |
PRINTS | PR00111; ABHYDROLASE; 1; |
PR00412; EPOXHYDRLASE; 1; | |
TIGRFAMs | TIGR01738; BioH; 1; |
From: BIOH_ECOLI (P13001) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 82 | 83 | Substrate binding | S-[LM] | ||||||||
REGION | 143 | 147 | Substrate binding | F-x-x-x-[QD] | ||||||||
ACT_SITE | 82 | 82 | Nucleophile | S | ||||||||
ACT_SITE | 207 | 207 | D | |||||||||
ACT_SITE | 235 | 235 | H | |||||||||
BINDING | 22 | 22 | Substrate; via amide nitrogen and carbonyl oxygen | W | ||||||||
BINDING | 235 | 235 | Substrate | H |
Size range | 239-312 amino acids |
Related rules | None |
Fusion | Nter: None; Cter: MF_00835 (BioC) |
Comments | Longer C-terminal in NEIMA; sequence not shown in alignment and not taken into account in size range |