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HAMAP rule MF_01260

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General rule information [?]

Accession MF_01260
Dates 26-JUL-2005 (Created)
1-JUN-2023 (Last updated, Version 26)
Name Carboxylester
Scope(s) Bacteria
Template(s) P13001 (BIOH_ECOLI); Q9K197 (BIOH_NEIMB); Q8GHL1 (BIOH_SERMA); [ Recover all ]
Triggered by HAMAP; MF_01260 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier BIOH
Protein name RecName: Full=Pimeloyl-[acyl-carrier protein] methyl ester esterase;
AltName: Full=Biotin synthesis protein BioH;
AltName: Full=Carboxylesterase BioH;
Gene name Name=bioH;

Comments [?]

FUNCTIONThe physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.
CATALYTIC ACTIVITY Reaction=6-carboxyhexanoyl-[ACP] methyl ester + H2O = 6- carboxyhexanoyl-[ACP] + H(+) + methanol; Xref=Rhea:RHEA:42700, Rhea:RHEA-COMP:9955, Rhea:RHEA-COMP:10186, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:78846, ChEBI:CHEBI:82735; EC=;
PATHWAYCofactor biosynthesis; biotin biosynthesis.
SIMILARITYBelongs to the AB hydrolase superfamily. Carboxylesterase BioH family.

Keywords [?]

Gene Ontology [?]

GO:0052689; Molecular function:carboxylic ester hydrolase activity
GO:0009102; Biological process:biotin biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00561; Abhydrolase_1; 1;
Pfam PF00975; Thioesterase; 1;
NCBIfam TIGR01738; BioH; 1;

Features [?]

From: BIOH_ECOLI (P13001)
Key From To Description Tag Condition FTGroup
BINDING 82 83 /ligand="substrate" S-[LM]
BINDING 143 147 /ligand="substrate" F-x-x-x-[QD]
ACT_SITE 82 82 /note="Nucleophile" S
ACT_SITE 207 207 D
ACT_SITE 235 235 H
BINDING 22 22 /ligand="substrate" W
BINDING 235 235 /ligand="substrate" H

Additional information [?]

Size range 239-312 amino acids
Related rules None
Fusion Nter: None Cter: MF_00835 (BioC)
Comments Longer C-terminal in NEIMA; sequence not shown in alignment and not taken into account in size range

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