HAMAP rule MF_01260
General rule information
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Accession | MF_01260 |
Dates | 26-JUL-2005 (Created) 1-JUN-2023 (Last updated, Version 26) |
Name | Carboxylester |
Scope | Bacteria; Pseudomonadota |
Templates | P13001 (BIOH_ECOLI); Q9K197 (BIOH_NEIMB); Q8GHL1 (BIOH_SERMA): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters. |
Catalytic activity | RHEA:42700: 6-carboxyhexanoyl-[ACP] methyl ester + H2O = 6-carboxyhexanoyl-[ACP] + H(+) + methanol
EC 3.1.1.85 |
Pathway | Cofactor biosynthesis; biotin biosynthesis. |
Subunit | Monomer. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family. |
Keywords
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Gene Ontology
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GO:0052689; Molecular function: carboxylic ester hydrolase activity.
GO:0009102; Biological process: biotin biosynthetic process.
GO:0005737; Cellular component: cytoplasm.
GO:0009102; Biological process: biotin biosynthetic process.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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Pfam | PF00561; Abhydrolase_1; 1; |
PF00975; Thioesterase; 1; | |
PRINTS | PR00111; ABHYDROLASE; 1; |
PR00412; EPOXHYDRLASE; 1; | |
NCBIfam | TIGR01738; BioH; 1; |
Features
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From: BIOH_ECOLI (P13001) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 82 | 83 | /ligand="substrate | S-[LM] | ||||||||
BINDING | 143 | 147 | /ligand="substrate | F-x-x-x-[QD] | ||||||||
ACT_SITE | 82 | 82 | Nucleophile | S | ||||||||
ACT_SITE | 207 | 207 | D | |||||||||
ACT_SITE | 235 | 235 | H | |||||||||
BINDING | 22 | 22 | /ligand="substrate | W | ||||||||
BINDING | 235 | 235 | /ligand="substrate | H |
Additional information
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Size range | 239-312 amino acids |
Related rules | None |
Fusion | Nter: None; Cter: MF_00835 (BioC) |
Comments | Longer C-terminal in NEIMA; sequence not shown in alignment and not taken into account in size range |