HAMAP rule MF_01260

General rule information [?]

Accession	MF_01260
Dates	26-JUL-2005 (Created) 1-JUN-2023 (Last updated, Version 26)

Name	Carboxylester

Scope

Bacteria; Pseudomonadota

Templates

P13001 (BIOH_ECOLI); Q9K197 (BIOH_NEIMB); Q8GHL1 (BIOH_SERMA): [Recover all]

Triggered by

HAMAP; MF_01260 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

BIOH

Protein name

RecName:		Full=Pimeloyl-[acyl-carrier protein] methyl ester esterase;
		EC 3.1.1.85;
AltName:		Full=Biotin synthesis protein BioH;
AltName:		Full=Carboxylesterase BioH;

Gene name

bioH

Comments [?]

Function	The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.
Catalytic activity	RHEA:42700: 6-carboxyhexanoyl-[ACP] methyl ester + H2O = 6-carboxyhexanoyl-[ACP] + H(+) + methanol EC 3.1.1.85
Pathway	Cofactor biosynthesis; biotin biosynthesis.
Subunit	Monomer.
Subcellular location	Cytoplasm.
Similarity	Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.

Keywords [?]

Cytoplasm
Biotin biosynthesis
Hydrolase
Serine esterase

Gene Ontology [?]

GO:0052689; Molecular function: carboxylic ester hydrolase activity.
GO:0009102; Biological process: biotin biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF00561; Abhydrolase_1; 1;
	PF00975; Thioesterase; 1;
PRINTS	PR00111; ABHYDROLASE; 1;
	PR00412; EPOXHYDRLASE; 1;
NCBIfam	TIGR01738; BioH; 1;

Features [?]

From: BIOH_ECOLI (P13001)

Key From To Description Tag Condition FTGroup

BINDING 82 83 /ligand="substrate S-[LM]

BINDING 143 147 /ligand="substrate F-x-x-x-[QD]

ACT_SITE 82 82 Nucleophile S

ACT_SITE 207 207 D

ACT_SITE 235 235 H

BINDING 22 22 /ligand="substrate W

BINDING 235 235 /ligand="substrate H

Additional information [?]

Size range	239-312 amino acids
Related rules	None
Fusion	Nter: None; Cter: MF_00835 (BioC)
Comments	Longer C-terminal in NEIMA; sequence not shown in alignment and not taken into account in size range

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