HAMAP rule MF_01261
General rule information
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Accession | MF_01261 |
Dates | 29-JUL-2005 (Created) 17-MAY-2023 (Last updated, Version 24) |
Name | CCA_bact_type1 |
Scope | Bacteria; Pseudomonadota |
Templates | P06961 (CCA_ECOLI); Q7SIB1 (CCA_GEOSE): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded. |
Catalytic activity | RHEA:14433: a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate
EC 2.7.7.72 |
RHEA:76235: a tRNA with a 3' CCA end + ATP + 2 CTP = a tRNA with a 3' CCACCA end + 3 diphosphate
PhysiologicalDirection=left-to-right (RHEA:76236) |
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Cofactor | Mg(2+) Note: Magnesium is required for nucleotidyltransferase activity. |
Ni(2+) Note: Nickel for phosphatase activity. |
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Subunit | Monomer. Can also form homodimers and oligomers. |
Domain | Comprises two domains: an N-terminal domain containing the nucleotidyltransferase activity and a C-terminal HD domain associated with both phosphodiesterase and phosphatase activities. |
Miscellaneous | A single active site specifically recognizes both ATP and CTP and is responsible for their addition. |
Similarity | Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily. |
Keywords
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ATP-binding
Hydrolase
Magnesium
Metal-binding
Multifunctional enzyme
Nickel
Nucleotide-binding
Nucleotidyltransferase
RNA repair
RNA-binding
Transferase
tRNA processing
Hydrolase
Magnesium
Metal-binding
Multifunctional enzyme
Nickel
Nucleotide-binding
Nucleotidyltransferase
RNA repair
RNA-binding
Transferase
tRNA processing
Gene Ontology
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GO:0000049; Molecular function: tRNA binding.
GO:0000166; Molecular function: nucleotide binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004112; Molecular function: cyclic-nucleotide phosphodiesterase activity.
GO:0004810; Molecular function: CCA tRNA nucleotidyltransferase activity.
GO:0005524; Molecular function: ATP binding.
GO:0016791; Molecular function: phosphatase activity.
GO:0001680; Biological process: tRNA 3'-terminal CCA addition.
GO:0000166; Molecular function: nucleotide binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004112; Molecular function: cyclic-nucleotide phosphodiesterase activity.
GO:0004810; Molecular function: CCA tRNA nucleotidyltransferase activity.
GO:0005524; Molecular function: ATP binding.
GO:0016791; Molecular function: phosphatase activity.
GO:0001680; Biological process: tRNA 3'-terminal CCA addition.
Cross-references
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Features
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From: CCA_ECOLI (P06961) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 21 | 21 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | [DE] | ||||||||
BINDING | 23 | 23 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | D | ||||||||
BINDING | 8 | 8 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | G | ||||||||
BINDING | 8 | 8 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | G | ||||||||
BINDING | 11 | 11 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | R | ||||||||
BINDING | 11 | 11 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | R | ||||||||
BINDING | 91 | 91 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | R | ||||||||
BINDING | 91 | 91 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | R | ||||||||
BINDING | 137 | 137 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | R | ||||||||
BINDING | 137 | 137 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | R | ||||||||
BINDING | 140 | 140 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | R | ||||||||
BINDING | 140 | 140 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | R |
Additional information
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Size range | 368-429 amino acids |
Related rules | MF_01262 (CCA) |
Fusion | None |
Comments | The CCA-adding enzyme family consists of 4 different subfamilies: 3 bacterial subfamilies (MF_01261, MF_01262 and MF_01263) and 1 archaeal subfamily (MF_01264). FT lines are propagated from G.stearothermophilus CCA-adding enzyme (type 3 subfamily, MF_01263) whose X-ray structure is known. |