HAMAP rule MF_01263

General rule information [?]

Accession	MF_01263
Dates	29-JUL-2005 (Created) 13-JAN-2023 (Last updated, Version 19)

Name	CCA_bact_type3

Scope

Bacteria; Bacillota

Templates

Q7SIB1 (CCA_GEOSE); P42977 (CCA_BACSU): [Recover all]

Triggered by

HAMAP; MF_01263 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

CCA

Protein name

RecName:		Full=CCA-adding enzyme;
		EC 2.7.7.72;
AltName:		Full=CCA tRNA nucleotidyltransferase;
AltName:		Full=tRNA CCA-pyrophosphorylase;
AltName:		Full=tRNA adenylyl-/cytidylyl- transferase;
AltName:		Full=tRNA nucleotidyltransferase;
AltName:		Full=tRNA-NT;

Gene name

cca

Comments [?]

Function	Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.
Catalytic activity	RHEA:14433: a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate EC 2.7.7.72
Cofactor	Mg(2+)
Subunit	Homodimer.
Miscellaneous	A single active site specifically recognizes both ATP and CTP and is responsible for their addition.
Similarity	Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.

Keywords [?]

ATP-binding
Magnesium
Metal-binding
Nucleotide-binding
Nucleotidyltransferase
RNA repair
RNA-binding
Transferase
tRNA processing

Gene Ontology [?]

GO:0000049; Molecular function: tRNA binding.
GO:0000166; Molecular function: nucleotide binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004810; Molecular function: CCA tRNA nucleotidyltransferase activity.
GO:0005524; Molecular function: ATP binding.
GO:0001680; Biological process: tRNA 3'-terminal CCA addition.

Cross-references [?]

Pfam	PF01743; PolyA_pol; 1;

Features [?]

From: CCA_GEOSE (Q7SIB1)

Key From To Description Tag Condition FTGroup

BINDING 40 40 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D

BINDING 42 42 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D

BINDING 27 27 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 G

BINDING 27 27 /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 G

BINDING 30 30 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 R

BINDING 30 30 /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 R

BINDING 111 111 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 R

BINDING 111 111 /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 R

BINDING 154 154 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 D

BINDING 154 154 /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 D

BINDING 157 157 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 R

BINDING 157 157 /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 R

BINDING 160 160 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 R

BINDING 160 160 /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 R

BINDING 163 163 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 R

BINDING 163 163 /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 R

Additional information [?]

Size range	375-407 amino acids
Related rules	None
Fusion	None
Comments	The CCA-adding enzyme family consists of 4 different subfamilies: 3 bacterial subfamilies (MF_01261, MF_01262 and MF_01263) and 1 archaeal subfamily (MF_01264).

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