HAMAP rule MF_01263
General rule information
[?]
Accession | MF_01263 |
Dates | 29-JUL-2005 (Created) 13-JAN-2023 (Last updated, Version 19) |
Name | CCA_bact_type3 |
Scope | Bacteria; Bacillota |
Templates | Q7SIB1 (CCA_GEOSE); P42977 (CCA_BACSU): [Recover all] |
Triggered by |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Identifier |
|
Protein name |
|
Gene name |
|
Comments
[?]
Function | Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. |
Catalytic activity | RHEA:14433: a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate
EC 2.7.7.72 |
Cofactor | Mg(2+) |
Subunit | Homodimer. |
Miscellaneous | A single active site specifically recognizes both ATP and CTP and is responsible for their addition. |
Similarity | Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily. |
Keywords
[?]
ATP-binding
Magnesium
Metal-binding
Nucleotide-binding
Nucleotidyltransferase
RNA repair
RNA-binding
Transferase
tRNA processing
Magnesium
Metal-binding
Nucleotide-binding
Nucleotidyltransferase
RNA repair
RNA-binding
Transferase
tRNA processing
Gene Ontology
[?]
GO:0000049; Molecular function: tRNA binding.
GO:0000166; Molecular function: nucleotide binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004810; Molecular function: CCA tRNA nucleotidyltransferase activity.
GO:0005524; Molecular function: ATP binding.
GO:0001680; Biological process: tRNA 3'-terminal CCA addition.
GO:0000166; Molecular function: nucleotide binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004810; Molecular function: CCA tRNA nucleotidyltransferase activity.
GO:0005524; Molecular function: ATP binding.
GO:0001680; Biological process: tRNA 3'-terminal CCA addition.
Cross-references
[?]
Pfam | PF01743; PolyA_pol; 1; |
Features
[?]
From: CCA_GEOSE (Q7SIB1) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 40 | 40 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | D | ||||||||
BINDING | 42 | 42 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | D | ||||||||
BINDING | 27 | 27 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | G | ||||||||
BINDING | 27 | 27 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | G | ||||||||
BINDING | 30 | 30 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | R | ||||||||
BINDING | 30 | 30 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | R | ||||||||
BINDING | 111 | 111 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | R | ||||||||
BINDING | 111 | 111 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | R | ||||||||
BINDING | 154 | 154 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | D | ||||||||
BINDING | 154 | 154 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | D | ||||||||
BINDING | 157 | 157 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | R | ||||||||
BINDING | 157 | 157 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | R | ||||||||
BINDING | 160 | 160 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | R | ||||||||
BINDING | 160 | 160 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | R | ||||||||
BINDING | 163 | 163 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | R | ||||||||
BINDING | 163 | 163 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | R |
Additional information
[?]
Size range | 375-407 amino acids |
Related rules | None |
Fusion | None |
Comments | The CCA-adding enzyme family consists of 4 different subfamilies: 3 bacterial subfamilies (MF_01261, MF_01262 and MF_01263) and 1 archaeal subfamily (MF_01264). |