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Annotation rule MF_01263 |
Accession | MF_01263 |
Dates | 29-JUL-2005 (Created) 19-NOV-2019 (Last updated, Version 14) |
Name | CCA_bact_type3 |
Scope | Bacteria; Firmicutes |
Templates | Q7SIB1 (CCA_GEOSE); P42977 (CCA_BACSU): [Recover all] |
Triggered by |
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Protein name |
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Gene name |
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Function | Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. |
Catalytic activity | RHEA:14433: a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate
EC 2.7.7.72 |
Cofactor | Mg(2+) |
Subunit | Homodimer. |
Miscellaneous | A single active site specifically recognizes both ATP and CTP and is responsible for their addition. |
Similarity | Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily. |
Pfam | PF01743; PolyA_pol; 1; |
From: CCA_GEOSE (Q7SIB1) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
METAL | 40 | 40 | Magnesium | D | ||||||||
METAL | 42 | 42 | Magnesium | D | ||||||||
BINDING | 27 | 27 | ATP or CTP; via amide nitrogen | G | ||||||||
BINDING | 30 | 30 | ATP or CTP | R | ||||||||
BINDING | 111 | 111 | ATP or CTP | R | ||||||||
BINDING | 154 | 154 | ATP or CTP | D | ||||||||
BINDING | 157 | 157 | ATP or CTP | R | ||||||||
BINDING | 160 | 160 | ATP or CTP | R | ||||||||
BINDING | 163 | 163 | ATP or CTP | R |
Size range | 375-407 amino acids |
Related rules | None |
Fusion | None |
Comments | The CCA-adding enzyme family consists of 4 different subfamilies: 3 bacterial subfamilies (MF_01261, MF_01262 and MF_01263) and 1 archaeal subfamily (MF_01264). |