HAMAP rule MF_01264
General rule information
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Accession | MF_01264 |
Dates | 29-JUL-2005 (Created) 1-JUN-2023 (Last updated, Version 19) |
Name | CCA_arch |
Scope | Archaea |
Templates | O28126 (CCA_ARCFU); P77978 (CCA_SACSH); Q58511 (CCA_METJA): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded. |
Catalytic activity | RHEA:14433: a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate
EC 2.7.7.72 |
RHEA:76235: a tRNA with a 3' CCA end + ATP + 2 CTP = a tRNA with a 3' CCACCA end + 3 diphosphate
PhysiologicalDirection=left-to-right (RHEA:76236) |
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Cofactor | Mg(2+) |
Subunit | Homodimer. |
Miscellaneous | A single active site specifically recognizes both ATP and CTP and is responsible for their addition. |
Similarity | Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily. |
Keywords
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ATP-binding
Magnesium
Metal-binding
Nucleotide-binding
Nucleotidyltransferase
RNA repair
RNA-binding
Transferase
tRNA processing
Magnesium
Metal-binding
Nucleotide-binding
Nucleotidyltransferase
RNA repair
RNA-binding
Transferase
tRNA processing
Gene Ontology
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GO:0000049; Molecular function: tRNA binding.
GO:0000166; Molecular function: nucleotide binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004810; Molecular function: CCA tRNA nucleotidyltransferase activity.
GO:0005524; Molecular function: ATP binding.
GO:0001680; Biological process: tRNA 3'-terminal CCA addition.
GO:0000166; Molecular function: nucleotide binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004810; Molecular function: CCA tRNA nucleotidyltransferase activity.
GO:0005524; Molecular function: ATP binding.
GO:0001680; Biological process: tRNA 3'-terminal CCA addition.
Cross-references
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Pfam | PF01909; NTP_transf_2; 1; |
PF09249; tRNA_NucTransf2; 1; | |
PIRSF | PIRSF005335; CCA_arch; 1; |
NCBIfam | TIGR03671; Cca_archaeal; 1; |
Features
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From: CCA_ARCFU (O28126) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 59 | 59 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | [DE] | ||||||||
BINDING | 61 | 61 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | D | ||||||||
BINDING | 110 | 110 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | [DE] | ||||||||
BINDING | 47 | 47 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | S | ||||||||
BINDING | 47 | 47 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | S | ||||||||
BINDING | 50 | 50 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | [RK] | ||||||||
BINDING | 50 | 50 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | [RK] | ||||||||
BINDING | 133 | 133 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | H | ||||||||
BINDING | 133 | 133 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | H | ||||||||
BINDING | 152 | 152 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | K | ||||||||
BINDING | 152 | 152 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | K | ||||||||
BINDING | 161 | 161 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | Y | ||||||||
BINDING | 161 | 161 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 | Y |
Additional information
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Size range | 392-485 amino acids |
Related rules | None |
Fusion | None |
Comments | The CCA-adding enzyme family consists of 4 different subfamilies: 3 bacterial subfamilies (MF_01261, MF_01262 and MF_01263) and 1 archaeal subfamily (MF_01264). |