HAMAP rule MF_01264
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_01264 |
| Accession | MF_01264 |
| Dates | 29-JUL-2005 (Created)
03-SEP-2024 (Last updated, Version 21) |
| Name | CCA_arch |
| Scope(s) |
Archaea |
| Template(s) | O28126; P77978; Q58511; [ Recover all ] |
| Triggered by |
HAMAP; MF_01264 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | CCA |
| Protein name | RecName: Full=CCA-adding enzyme; EC=2.7.7.72; AltName: Full=CCA tRNA nucleotidyltransferase; AltName: Full=tRNA CCA-pyrophosphorylase; AltName: Full=tRNA adenylyl-/cytidylyl- transferase; AltName: Full=tRNA nucleotidyltransferase; AltName: Full=tRNA-NT; |
| Gene name | Name=cca; |
Comments
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| FUNCTION | Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded. |
| CATALYTIC ACTIVITY | Reaction=a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate; Xref=Rhea:RHEA:14433, Rhea:RHEA-COMP:10465, Rhea:RHEA- COMP:10468, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:37563, ChEBI:CHEBI:74896, ChEBI:CHEBI:83071; EC=2.7.7.72; |
| CATALYTIC ACTIVITY | Reaction=a tRNA with a 3' CCA end + 2 CTP + ATP = a tRNA with a 3' CCACCA end + 3 diphosphate; Xref=Rhea:RHEA:76235, Rhea:RHEA- COMP:10468, Rhea:RHEA-COMP:18655, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:37563, ChEBI:CHEBI:83071, ChEBI:CHEBI:195187; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:76236; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| SUBUNIT | Homodimer. |
| MISCELLANEOUS | A single active site specifically recognizes both ATP and CTP and is responsible for their addition. |
| SIMILARITY | Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily. |
Keywords
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| ATP-binding |
| Magnesium |
| Metal-binding |
| Nucleotide-binding |
| Nucleotidyltransferase |
| RNA repair |
| RNA-binding |
| Transferase |
| tRNA processing |
Gene Ontology
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| GO:0000049; Molecular function:tRNA binding |
| GO:0000166; Molecular function:nucleotide binding |
| GO:0000287; Molecular function:magnesium ion binding |
| GO:0004810; Molecular function:CCA tRNA nucleotidyltransferase activity |
| GO:0005524; Molecular function:ATP binding |
| GO:0001680; Biological process:tRNA 3'-terminal CCA addition |
Cross-references
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| Pfam | PF01909; NTP_transf_2; 1; |
| Pfam | PF09249; tRNA_NucTransf2; 1; |
| PIRSF | PIRSF005335; CCA_arch; 1; |
| NCBIfam | TIGR03671; Cca_archaeal; 1; |
Features
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| From: CCA_ARCFU (O28126) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 59 | 59 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[DE] | ||||||||
| BINDING | 61 | 61 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 110 | 110 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[DE] | ||||||||
| BINDING | 47 | 47 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
S | ||||||||
| BINDING | 47 | 47 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563" |
S | ||||||||
| BINDING | 50 | 50 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[RK] | ||||||||
| BINDING | 50 | 50 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563" |
[RK] | ||||||||
| BINDING | 133 | 133 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
H | ||||||||
| BINDING | 133 | 133 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563" |
H | ||||||||
| BINDING | 152 | 152 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
| BINDING | 152 | 152 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563" |
K | ||||||||
| BINDING | 161 | 161 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
Y | ||||||||
| BINDING | 161 | 161 | /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563" |
Y | ||||||||
Additional information
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| Size range | 392-485 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | The CCA-adding enzyme family consists of 4 different subfamilies: 3 bacterial subfamilies (MF_01261, MF_01262 and MF_01263) and 1 archaeal subfamily (MF_01264). |