HAMAP rule MF_01264

General rule information [?]

Accession	MF_01264
Dates	29-JUL-2005 (Created) 1-JUN-2023 (Last updated, Version 19)

Name

CCA_arch

Scope

Archaea

Templates

O28126 (CCA_ARCFU); P77978 (CCA_SACSH); Q58511 (CCA_METJA): [Recover all]

Triggered by

HAMAP; MF_01264 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

CCA

Protein name

RecName:		Full=CCA-adding enzyme;
		EC 2.7.7.72;
AltName:		Full=CCA tRNA nucleotidyltransferase;
AltName:		Full=tRNA CCA-pyrophosphorylase;
AltName:		Full=tRNA adenylyl-/cytidylyl- transferase;
AltName:		Full=tRNA nucleotidyltransferase;
AltName:		Full=tRNA-NT;

Gene name

cca

Comments [?]

Function	Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.
Catalytic activity	RHEA:14433: a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate EC 2.7.7.72
	RHEA:76235: a tRNA with a 3' CCA end + ATP + 2 CTP = a tRNA with a 3' CCACCA end + 3 diphosphate PhysiologicalDirection=left-to-right (RHEA:76236)
Cofactor	Mg(2+)
Subunit	Homodimer.
Miscellaneous	A single active site specifically recognizes both ATP and CTP and is responsible for their addition.
Similarity	Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.

Keywords [?]

ATP-binding
Magnesium
Metal-binding
Nucleotide-binding
Nucleotidyltransferase
RNA repair
RNA-binding
Transferase
tRNA processing

Gene Ontology [?]

GO:0000049; Molecular function: tRNA binding.
GO:0000166; Molecular function: nucleotide binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004810; Molecular function: CCA tRNA nucleotidyltransferase activity.
GO:0005524; Molecular function: ATP binding.
GO:0001680; Biological process: tRNA 3'-terminal CCA addition.

Cross-references [?]

Pfam	PF01909; NTP_transf_2; 1;
	PF09249; tRNA_NucTransf2; 1;
PIRSF	PIRSF005335; CCA_arch; 1;
NCBIfam	TIGR03671; Cca_archaeal; 1;

Features [?]

From: CCA_ARCFU (O28126)

Key From To Description Tag Condition FTGroup

BINDING 59 59 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 [DE]

BINDING 61 61 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D

BINDING 110 110 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 [DE]

BINDING 47 47 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 S

BINDING 47 47 /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 S

BINDING 50 50 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [RK]

BINDING 50 50 /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 [RK]

BINDING 133 133 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 H

BINDING 133 133 /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 H

BINDING 152 152 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 K

BINDING 152 152 /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 K

BINDING 161 161 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 Y

BINDING 161 161 /ligand="CTP" /ligand_id="ChEBI:CHEBI:37563 Y

Additional information [?]

Size range	392-485 amino acids
Related rules	None
Fusion	None
Comments	The CCA-adding enzyme family consists of 4 different subfamilies: 3 bacterial subfamilies (MF_01261, MF_01262 and MF_01263) and 1 archaeal subfamily (MF_01264).

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