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Annotation rule MF_01264
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General rule information [?]

Accession MF_01264
Dates 29-JUL-2005 (Created)
19-NOV-2019 (Last updated, Version 12)
Name CCA_arch
Templates O28126 (CCA_ARCFU); P77978 (CCA_SACSH); Q58511 (CCA_METJA): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=CCA-adding enzyme;
AltName: Full=CCA tRNA nucleotidyltransferase;
AltName: Full=tRNA CCA-pyrophosphorylase;
AltName: Full=tRNA adenylyl-/cytidylyl- transferase;
AltName: Full=tRNA nucleotidyltransferase;
AltName: Full=tRNA-NT;
Gene name

Comments [?]

Function Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.
Catalytic activity RHEA:14433: a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate
Cofactor Mg(2+)
Subunit Homodimer.
Miscellaneous A single active site specifically recognizes both ATP and CTP and is responsible for their addition.
Similarity Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.

Keywords [?]

Gene Ontology [?]

GO:0000049; Molecular function: tRNA binding.
GO:0000166; Molecular function: nucleotide binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004810; Molecular function: tRNA adenylyltransferase activity.
GO:0005524; Molecular function: ATP binding.
GO:0016437; Molecular function: tRNA cytidylyltransferase activity.
GO:0001680; Biological process: tRNA 3'-terminal CCA addition.

Cross-references [?]

Pfam PF01909; NTP_transf_2; 1;
PF09249; tRNA_NucTransf2; 1;
PIRSF PIRSF005335; CCA_arch; 1;
TIGRFAMs TIGR03671; Cca_archaeal; 1;

Features [?]

From: CCA_ARCFU (O28126)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     59     59       Magnesium     [DE]  
METAL     61     61       Magnesium     D  
METAL     110     110       Magnesium     [DE]  
BINDING     47     47       ATP or CTP     S  
BINDING     50     50       ATP or CTP     [RK]  
BINDING     133     133       ATP or CTP     H  
BINDING     152     152       ATP or CTP     K  
BINDING     161     161       ATP or CTP     Y  

Additional information [?]

Size range 392-485 amino acids
Related rules None
Fusion None
Comments The CCA-adding enzyme family consists of 4 different subfamilies: 3 bacterial subfamilies (MF_01261, MF_01262 and MF_01263) and 1 archaeal subfamily (MF_01264).