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Annotation rule MF_01264 |
Accession | MF_01264 |
Dates | 29-JUL-2005 (Created) 19-NOV-2019 (Last updated, Version 12) |
Name | CCA_arch |
Scope | Archaea |
Templates | O28126 (CCA_ARCFU); P77978 (CCA_SACSH); Q58511 (CCA_METJA): [Recover all] |
Triggered by |
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Protein name |
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Gene name |
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Function | Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. |
Catalytic activity | RHEA:14433: a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate
EC 2.7.7.72 |
Cofactor | Mg(2+) |
Subunit | Homodimer. |
Miscellaneous | A single active site specifically recognizes both ATP and CTP and is responsible for their addition. |
Similarity | Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily. |
Pfam | PF01909; NTP_transf_2; 1; |
PF09249; tRNA_NucTransf2; 1; | |
PIRSF | PIRSF005335; CCA_arch; 1; |
TIGRFAMs | TIGR03671; Cca_archaeal; 1; |
From: CCA_ARCFU (O28126) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
METAL | 59 | 59 | Magnesium | [DE] | ||||||||
METAL | 61 | 61 | Magnesium | D | ||||||||
METAL | 110 | 110 | Magnesium | [DE] | ||||||||
BINDING | 47 | 47 | ATP or CTP | S | ||||||||
BINDING | 50 | 50 | ATP or CTP | [RK] | ||||||||
BINDING | 133 | 133 | ATP or CTP | H | ||||||||
BINDING | 152 | 152 | ATP or CTP | K | ||||||||
BINDING | 161 | 161 | ATP or CTP | Y |
Size range | 392-485 amino acids |
Related rules | None |
Fusion | None |
Comments | The CCA-adding enzyme family consists of 4 different subfamilies: 3 bacterial subfamilies (MF_01261, MF_01262 and MF_01263) and 1 archaeal subfamily (MF_01264). |