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Annotation rule MF_01268
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General rule information [?]

Accession MF_01268
Dates 2-MAY-2006 (Created)
19-NOV-2019 (Last updated, Version 16)
Name Fae_Hps
Templates Q46DY5 (FAEHP_METBF); Q58842 (FAEHP_METJA): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Bifunctional enzyme Fae/Hps;
RecName: Full=5,6,7,8-tetrahydromethanopterin hydro-lyase;
AltName: Full=Formaldehyde-activating enzyme;
RecName: Full=3-hexulose-6-phosphate synthase;
AltName: Full=D-arabino-3-hexulose-6-phosphate formaldehyde lyase;
Gene name

Comments [?]

Function Catalyzes the condensation of formaldehyde with tetrahydromethanopterin (H(4)MPT) to 5,10-methylenetetrahydromethanopterin.
Catalyzes the reversible formation of ribulose-5-phosphate and formaldehyde from 3-hexulose-6-phosphate.
Catalytic activity RHEA:24678: 5,6,7,8-tetrahydromethanopterin + formaldehyde = 5,10-methylenetetrahydromethanopterin + H2O
RHEA:25201: D-ribulose 5-phosphate + formaldehyde = D-arabino-hex-3-ulose 6-phosphate
Pathway Carbohydrate biosynthesis; D-ribose 5-phosphate biosynthesis.
Similarity In the N-terminal section; belongs to the formaldehyde-activating enzyme family.
In the C-terminal section; belongs to the HPS/KGPDC family. HPS subfamily.

Keywords [?]

Gene Ontology [?]

GO:0043801; Molecular function: hexulose-6-phosphate synthase activity.
GO:0016836; Molecular function: hydro-lyase activity.
GO:0016051; Biological process: carbohydrate biosynthetic process.

Cross-references [?]

Pfam PF00215; OMPdecase; 1;
TIGRFAMs TIGR03126; one_C_fae; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
REGION     Nter     161       Formaldehyde-activating enzyme        
REGION     162     Cter       3-hexulose-6-phosphate synthase        
ACT_SITE     17     17       Proton donor     H  
BINDING     19     19       Substrate     D  
BINDING     48     48       Substrate; via carbonyl oxygen     L  
BINDING     66     66       Substrate     K  
BINDING     68     68       Substrate     T  
BINDING     83     83       Substrate     Q  

Additional information [?]

Size range 375-420 amino acids
Related rules None
Fusion None
Comments In METJA, lacks the active site His and substrate binding sites expected to be involved in fae activity. Monofunctional fae enzyme exists in methylotrophic bacteria, where it is involved in energy metabolism and formaldehyde detoxification. Monofunctional HPS enzyme exists in bacteria, but functions in the opposite direction (condensation of ribulose 5-phosphate with formaldehyde), and is involved in C1 assimilation and/or formaldehyde detoxification. METBF possesses the bifunctional fae/hps enzyme, and also the two monofunctional enzymes fae and hps. FT lines are propagated from Methylobacterium extorquens fae enzyme, whose X-ray structure is known.