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HAMAP rule MF_01268

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General rule information [?]

Accession MF_01268
Dates 2-MAY-2006 (Created)
1-JUN-2023 (Last updated, Version 18)
Name Fae_Hps
Scope(s) Archaea
Template(s) Q46DY5 (FAEHP_METBF); Q58842 (FAEHP_METJA); [ Recover all ]
Triggered by HAMAP; MF_01268 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier FAEHP
Protein name RecName: Full=Bifunctional enzyme Fae/Hps;
RecName: Full=5,6,7,8-tetrahydromethanopterin hydro-lyase;
AltName: Full=Formaldehyde-activating enzyme;
RecName: Full=3-hexulose-6-phosphate synthase;
AltName: Full=D-arabino-3-hexulose-6-phosphate formaldehyde lyase;
Gene name Name=fae-hps;

Comments [?]

FUNCTIONCatalyzes the condensation of formaldehyde with tetrahydromethanopterin (H(4)MPT) to 5,10- methylenetetrahydromethanopterin.
FUNCTIONCatalyzes the reversible formation of ribulose-5-phosphate and formaldehyde from 3-hexulose-6-phosphate.
CATALYTIC ACTIVITY Reaction=5,6,7,8-tetrahydromethanopterin + formaldehyde = 5,10- methylenetetrahydromethanopterin + H2O; Xref=Rhea:RHEA:24678, ChEBI:CHEBI:15377, ChEBI:CHEBI:16842, ChEBI:CHEBI:57818, ChEBI:CHEBI:58103; EC=;
CATALYTIC ACTIVITY Reaction=D-ribulose 5-phosphate + formaldehyde = D-arabino-hex-3-ulose 6-phosphate; Xref=Rhea:RHEA:25201, ChEBI:CHEBI:16842, ChEBI:CHEBI:58121, ChEBI:CHEBI:58542; EC=;
PATHWAYCarbohydrate biosynthesis; D-ribose 5-phosphate biosynthesis.
SIMILARITYIn the N-terminal section; belongs to the formaldehyde- activating enzyme family.
SIMILARITYIn the C-terminal section; belongs to the HPS/KGPDC family. HPS subfamily.

Keywords [?]

Gene Ontology [?]

GO:0043801; Molecular function:hexulose-6-phosphate synthase activity
GO:0016836; Molecular function:hydro-lyase activity
GO:0016051; Biological process:carbohydrate biosynthetic process

Cross-references [?]

Pfam PF00215; OMPdecase; 1;
NCBIfam TIGR03126; one_C_fae; 1;

Features [?]

Key From To Description Tag Condition FTGroup
REGION Nter 161 /note="Formaldehyde-activating enzyme"
REGION 162 Cter /note="3-hexulose-6-phosphate synthase"
ACT_SITE 17 17 /note="Proton donor" H
BINDING 19 19 /ligand="substrate" D
BINDING 48 48 /ligand="substrate" L
BINDING 66 66 /ligand="substrate" K
BINDING 68 68 /ligand="substrate" T
BINDING 83 83 /ligand="substrate" Q

Additional information [?]

Size range 375-420 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments In METJA, lacks the active site His and substrate binding sites expected to be involved in fae activity. Monofunctional fae enzyme exists in methylotrophic bacteria, where it is involved in energy metabolism and formaldehyde detoxification. Monofunctional HPS enzyme exists in bacteria, but functions in the opposite direction (condensation of ribulose 5-phosphate with formaldehyde), and is involved in C1 assimilation and/or formaldehyde detoxification. METBF possesses the bifunctional fae/hps enzyme, and also the two monofunctional enzymes fae and hps. FT lines are propagated from Methylobacterium extorquens fae enzyme, whose X-ray structure is known.

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