HAMAP rule MF_01269

General rule information [?]

Accession	MF_01269
Dates	18-MAY-2006 (Created) 19-NOV-2022 (Last updated, Version 21)

Name	Shikimate_kinase_2

Scope

Bacteria; Enterobacterales

Templates

P10880 (AROL_DICCH); P0A6E1 (AROL_ECOLI): [Recover all]

Triggered by

HAMAP; MF_01269 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

AROL

Protein name

RecName:		Full=Shikimate kinase 2;
		Short=SK 2;
		EC 2.7.1.71;

Gene name

aroL

Comments [?]

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.
Catalytic activity	RHEA:13121: ATP + shikimate = 3-phosphoshikimate + ADP + H(+) EC 2.7.1.71
Cofactor	Mg(2+) Note: Binds 1 Mg(2+) ion per subunit.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.
Subunit	Monomer.
Subcellular location	Cytoplasm.
Domain	The LID domain closes over the active site upon ATP binding.
Similarity	Belongs to the shikimate kinase family. AroL subfamily.

Keywords [?]

Cytoplasm
Amino-acid biosynthesis
Aromatic amino acid biosynthesis
ATP-binding
Kinase
Magnesium
Metal-binding
Nucleotide-binding
Transferase

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0004765; Molecular function: shikimate kinase activity.
GO:0009073; Biological process: aromatic amino acid family biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE	PS01128; SHIKIMATE_KINASE; 1;
Pfam	PF01202; SKI; 1;
PRINTS	PR01100; SHIKIMTKNASE; 1;

Features [?]

From: AROL_DICCH (P10880)

Key From To Description Tag Condition FTGroup

BINDING 12 17 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 G-[CA]-G-K-T-T

REGION 112 126 LID domain

BINDING 16 16 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 T

BINDING 32 32 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D

BINDING 34 34 /ligand="substrate D

BINDING 58 58 /ligand="substrate R

BINDING 79 79 /ligand="substrate G

BINDING 120 120 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 R

BINDING 139 139 /ligand="substrate R

BINDING (Optional) 155 155 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 Q

Additional information [?]

Size range	173-181 amino acids
Related rules	MF_00109 (AROK)
Fusion	None
Comments	Mg(2+) is six-coordinated in shikimate kinase 1 (aroK, MF_00109) with direct interaction with two protein side-chains, whereas it is four-coordinated in shikimate kinase 2 (aroL, MF_01269) with direct interaction with only one protein side-chain.

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