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Annotation rule MF_01269 |
Accession | MF_01269 |
Dates | 18-MAY-2006 (Created) 19-NOV-2019 (Last updated, Version 19) |
Name | Shikimate_kinase_2 |
Scope | Bacteria; Enterobacterales |
Templates | P10880 (AROL_DICCH); P0A6E1 (AROL_ECOLI): [Recover all] |
Triggered by |
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Protein name |
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Gene name |
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Function | Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate. |
Catalytic activity | RHEA:13121: ATP + shikimate = 3-phosphoshikimate + ADP + H(+)
EC 2.7.1.71 |
Cofactor | Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
Pathway | Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. |
Subunit | Monomer. |
Subcellular location | Cytoplasm. |
Domain | The LID domain closes over the active site upon ATP binding. |
Similarity | Belongs to the shikimate kinase family. AroL subfamily. |
From: AROL_DICCH (P10880) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
NP_BIND | 12 | 17 | ATP | G-[CA]-G-K-T-T | ||||||||
REGION | 112 | 126 | LID domain | |||||||||
METAL | 16 | 16 | Magnesium | T | ||||||||
METAL | 32 | 32 | Magnesium | D | ||||||||
BINDING | 34 | 34 | Substrate | D | ||||||||
BINDING | 58 | 58 | Substrate | R | ||||||||
BINDING | 79 | 79 | Substrate; via amide nitrogen | G | ||||||||
BINDING | 120 | 120 | ATP | R | ||||||||
BINDING | 139 | 139 | Substrate | R | ||||||||
BINDING (Optional) | 155 | 155 | ATP | Q |
Size range | 173-181 amino acids |
Related rules | MF_00109 (AROK) |
Fusion | None |
Comments | Mg(2+) is six-coordinated in shikimate kinase 1 (aroK, MF_00109) with direct interaction with two protein side-chains, whereas it is four-coordinated in shikimate kinase 2 (aroL, MF_01269) with direct interaction with only one protein side-chain. |