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Annotation rule MF_01269
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General rule information [?]

Accession MF_01269
Dates 18-MAY-2006 (Created)
19-NOV-2019 (Last updated, Version 19)
Name Shikimate_kinase_2
Scope
Bacteria; Enterobacterales
Templates P10880 (AROL_DICCH); P0A6E1 (AROL_ECOLI): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
AROL
Protein name
RecName: Full=Shikimate kinase 2;
Short=SK 2;
EC 2.7.1.71;
Gene name
aroL

Comments [?]

Function Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.
Catalytic activity RHEA:13121: ATP + shikimate = 3-phosphoshikimate + ADP + H(+)
EC 2.7.1.71
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
Pathway Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.
Subunit Monomer.
Subcellular location Cytoplasm.
Domain The LID domain closes over the active site upon ATP binding.
Similarity Belongs to the shikimate kinase family. AroL subfamily.

Keywords [?]

Cytoplasm
Amino-acid biosynthesis
Aromatic amino acid biosynthesis
ATP-binding
Kinase
Magnesium
Metal-binding
Nucleotide-binding
Transferase

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0004765; Molecular function: shikimate kinase activity.
GO:0009073; Biological process: aromatic amino acid family biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS01128; SHIKIMATE_KINASE; 1;
Pfam PF01202; SKI; 1;
PRINTS PR01100; SHIKIMTKNASE; 1;

Features [?]

From: AROL_DICCH (P10880)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     12     17       ATP     G-[CA]-G-K-T-T  
REGION     112     126       LID domain        
METAL     16     16       Magnesium     T  
METAL     32     32       Magnesium     D  
BINDING     34     34       Substrate     D  
BINDING     58     58       Substrate     R  
BINDING     79     79       Substrate; via amide nitrogen     G  
BINDING     120     120       ATP     R  
BINDING     139     139       Substrate     R  
BINDING (Optional)     155     155       ATP     Q  

Additional information [?]

Size range 173-181 amino acids
Related rules MF_00109 (AROK)
Fusion None
Comments Mg(2+) is six-coordinated in shikimate kinase 1 (aroK, MF_00109) with direct interaction with two protein side-chains, whereas it is four-coordinated in shikimate kinase 2 (aroL, MF_01269) with direct interaction with only one protein side-chain.


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