HAMAP rule MF_01279

General rule information [?]

Accession	MF_01279
Dates	15-AUG-2007 (Created) 19-NOV-2022 (Last updated, Version 15)

Name	X_Pro_dipeptid

Scope

Bacteria; Gammaproteobacteria

Templates

P21165 (PEPQ_ECOLI); Q44238 (PEPQ_ALTSX); P77814 (PEPQ_PSEHA): [Recover all]

Triggered by

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PEPQ

Protein name

Gene name

pepQ

Comments [?]

Function	Splits dipeptides with a prolyl residue in the C-terminal position.
Catalytic activity	Reaction=Hydrolysis of Xaa-\|-Pro dipeptides. Also acts on aminoacyl-hydroxyproline analogs. No action on Pro-\|-Pro.; EC=3.4.13.9;
Cofactor	Mn(2+) Note: Binds 2 manganese ions per subunit.
Similarity	Belongs to the peptidase M24B family. Bacterial-type prolidase subfamily.

Keywords [?]

Gene Ontology [?]

GO:0008235; Molecular function: metalloexopeptidase activity.
GO:0016805; Molecular function: dipeptidase activity.

Cross-references [?]

PROSITE	PS00491; PROLINE_PEPTIDASE; 1;
Pfam	PF00557; Peptidase_M24; 1;

Features [?]

From: PEPQ_ECOLI (P21165)

Key From To Description Tag Condition FTGroup

BINDING 246 246 /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="2 D

BINDING 257 257 /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1 D

BINDING 257 257 /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="2 D

BINDING 339 339 /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1 H

BINDING 384 384 /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1 E

BINDING 423 423 /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1 E

BINDING 423 423 /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="2 E

Additional information [?]

Size range	429-452 amino acids
Related rules	None
Fusion	None
Comments	PepQ from Alteromonas sp. and Pseudoalteromonas haloplanktis was also shown to efficiently catalyze the hydrolysis of toxic organophosphorus cholinesterase-inhibiting compounds including insecticide paraoxon and nerve gases such as O-isopropyl methylphosphonofluoridate (sarin) and O-pinacolyl (soman).