HAMAP rule MF_01281
General rule information
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Accession | MF_01281 |
Dates | 7-NOV-2007 (Created)
19-NOV-2022 (Last updated, Version 25) |
Name | MTA_SAH_deamin |
Scope(s) |
Bacteria Archaea |
Template(s) | Q9X034 (MTAD_THEMA); Q58936 (DADD_METJA); [ Recover all ] |
Triggered by |
HAMAP; MF_01281 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
Identifier | DADD |
Protein name | RecName: Full=5'-deoxyadenosine deaminase; Short=5'-dA deaminase; EC=3.5.4.41; AltName: Full=5'-methylthioadenosine deaminase; Short=MTA deaminase; EC=3.5.4.31; AltName: Full=Adenosine deaminase; EC=3.5.4.4; AltName: Full=S-adenosylhomocysteine deaminase; Short=SAH deaminase; EC=3.5.4.28; |
Gene name | Name=dadD; |
else | |
Identifier | MTAD |
Protein name | RecName: Full=5-methylthioadenosine/S-adenosylhomocysteine deaminase; Short=MTA/SAH deaminase; EC=3.5.4.28; EC=3.5.4.31; |
Gene name | Name=mtaD; |
end case |
Comments
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case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
FUNCTION | Catalyzes the deamination of three SAM-derived enzymatic products, namely 5'-deoxyadenosine, S-adenosyl-L-homocysteine, and 5'- methylthioadenosine, to produce the inosine analogs. Can also deaminate adenosine. The preferred substrate for this enzyme is 5'- deoxyadenosine, but all these substrates are efficiently deaminated. Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions. May also be involved in the recycling of 5'- deoxyadenosine, whereupon the 5'-deoxyribose moiety of 5'-deoxyinosine is further metabolized to deoxyhexoses used for the biosynthesis of aromatic amino acids in methanogens. |
CATALYTIC ACTIVITY | Reaction=5'-deoxyadenosine + H(+) + H2O = 5'-deoxyinosine + NH4(+); Xref=Rhea:RHEA:42892, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:28938, ChEBI:CHEBI:82775; EC=3.5.4.41; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42893; |
CATALYTIC ACTIVITY | Reaction=H(+) + H2O + S-adenosyl-L-homocysteine = NH4(+) + S-inosyl-L- homocysteine; Xref=Rhea:RHEA:20716, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:57856, ChEBI:CHEBI:57985; EC=3.5.4.28; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20717; |
CATALYTIC ACTIVITY | Reaction=H(+) + H2O + S-methyl-5'-thioadenosine = NH4(+) + S-methyl-5'- thioinosine; Xref=Rhea:RHEA:25025, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:28938, ChEBI:CHEBI:48595; EC=3.5.4.31; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25026; |
CATALYTIC ACTIVITY | Reaction=adenosine + H(+) + H2O = inosine + NH4(+); Xref=Rhea:RHEA:24408, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16335, ChEBI:CHEBI:17596, ChEBI:CHEBI:28938; EC=3.5.4.4; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:24409; |
PATHWAY | Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis. |
SUBUNIT | Homotetramer. |
MISCELLANEOUS | SAH is a product of SAM methyltransferases and is known to be a feedback inhibitor of these enzymes. As a result of this inhibition, organisms have evolved efficient enzymes to metabolize SAH via different pathways. The pathway found in methanogens differs from the canonical pathway, it uses the deamination of S-adenosyl-L- homocysteine to form S-inosyl-L-homocysteine for the regeneration of SAM from S-adenosyl-L-homocysteine. 5'-deoxyadenosine is a radical SAM enzyme reaction product which strongly inhibits radical SAM enzymes. A pathway for removing this product must be present in methanogens where the MTA/SAH nucleosidase which normally metabolizes this compound is absent. |
else | |
FUNCTION | Catalyzes the deamination of 5-methylthioadenosine and S- adenosyl-L-homocysteine into 5-methylthioinosine and S-inosyl-L- homocysteine, respectively. Is also able to deaminate adenosine. |
CATALYTIC ACTIVITY | Reaction=H(+) + H2O + S-adenosyl-L-homocysteine = NH4(+) + S-inosyl-L- homocysteine; Xref=Rhea:RHEA:20716, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:57856, ChEBI:CHEBI:57985; EC=3.5.4.28; |
CATALYTIC ACTIVITY | Reaction=H(+) + H2O + S-methyl-5'-thioadenosine = NH4(+) + S-methyl-5'- thioinosine; Xref=Rhea:RHEA:25025, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:28938, ChEBI:CHEBI:48595; EC=3.5.4.31; |
end case | |
COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
SIMILARITY | Belongs to the metallo-dependent hydrolases superfamily. MTA/SAH deaminase family. |
Keywords
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Gene Ontology
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case <OCellular component:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
GO:0090613; Molecular function:5'-deoxyadenosine deaminase activity | |
GO:0004000; Molecular function:adenosine deaminase activity | |
else; https://www.ebi.ac.uk/QuickGO/term/else | |
GO:0050270; Molecular function:S-adenosylhomocysteine deaminase activity | |
GO:0090614; Molecular function:5'-methylthioadenosine deaminase activity | |
end case |
Cross-references
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Pfam | PF01979; Amidohydro_1; 1; |
Features
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From: MTAD_THEMA (Q9X034) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 55 | 55 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | ||||||||
BINDING | 57 | 57 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | ||||||||
BINDING | 200 | 200 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | ||||||||
BINDING | 279 | 279 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
D | ||||||||
BINDING | 84 | 84 | /ligand="substrate" | E | ||||||||
BINDING | 136 | 136 | /ligand="substrate" | R | ||||||||
BINDING | 148 | 148 | /ligand="substrate" | R | ||||||||
BINDING | 173 | 173 | /ligand="substrate" | H | ||||||||
BINDING | 203 | 203 | /ligand="substrate" | E | ||||||||
BINDING | 279 | 279 | /ligand="substrate" | D |
Additional information
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Size range | 406-449 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |