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HAMAP rule MF_01287

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General rule information [?]

Accession MF_01287
Dates 20-AUG-2008 (Created)
1-JUN-2023 (Last updated, Version 22)
Name DGGGPL_reductase
Scope
Archaea
Templates O29786 (GGR_ARCFU); Q9HKS9 (GGR_THEAC): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
GGR
case <OC:Thermoplasmata>
Protein name
RecName: Full=Digeranylgeranylglycerophospholipid reductase;
Short=DGGGPL reductase;
EC 1.3.1.101;
AltName: Full=2,3-bis-O-geranylgeranylglyceryl phosphate reductase;
AltName: Full=Geranylgeranyl reductase;
Short=GGR;
else case <OC:Methanomicrobia>
Protein name
RecName: Full=Digeranylgeranylglycerophospholipid reductase;
Short=DGGGPL reductase;
EC 1.3.7.11;
AltName: Full=2,3-bis-O-geranylgeranylglyceryl phosphate reductase;
AltName: Full=Geranylgeranyl reductase;
Short=GGR;
else
Protein name
RecName: Full=Digeranylgeranylglycerophospholipid reductase;
Short=DGGGPL reductase;
EC 1.3.-.-;
AltName: Full=2,3-bis-O-geranylgeranylglyceryl phosphate reductase;
AltName: Full=Geranylgeranyl reductase;
Short=GGR;
end case

Comments [?]

Function Is involved in the reduction of 2,3-digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3-diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids. Catalyzes the formation of archaetidic acid (2,3-di-O-phytanyl-sn-glyceryl phosphate) from 2,3-di-O-geranylgeranylglyceryl phosphate (DGGGP) via the hydrogenation of each double bond of the isoprenoid chains.
case <OC:Thermoplasmata>
Catalytic activity RHEA:36035: 2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 NADP(+) = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 H(+) + 8 NADPH
EC 1.3.1.101
PhysiologicalDirection=right-to-left (RHEA:36037)
RHEA:36039: 2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 NAD(+) = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 H(+) + 8 NADH
EC 1.3.1.101
PhysiologicalDirection=right-to-left (RHEA:36041)
else case <OC:Methanomicrobia>
Catalytic activity RHEA:54324: a 2,3-bis-O-phytanyl-sn-glycerol 1-phospholipid + 8 oxidized 2[4Fe-4S]-[ferredoxin] = a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid + 16 H(+) + 8 reduced 2[4Fe-4S]-[ferredoxin]
EC 1.3.7.11
PhysiologicalDirection=right-to-left (RHEA:54326)
RHEA:36159: 2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 oxidized 2[4Fe-4S]-[ferredoxin] = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 16 H(+) + 8 reduced 2[4Fe-4S]-[ferredoxin]
EC 1.3.7.11
PhysiologicalDirection=right-to-left (RHEA:36161)
else
Catalytic activity RHEA:64376: 8 A + a 2,3-bis-O-phytanyl-sn-glycerol 1-phospholipid = a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid + 8 AH2
PhysiologicalDirection=right-to-left (RHEA:64378)
RHEA:64368: 2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 A = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 AH2
PhysiologicalDirection=right-to-left (RHEA:64370)
end case
Cofactor FAD
Note: Binds 1 FAD per subunit.
Pathway Membrane lipid metabolism; glycerophospholipid metabolism.
Miscellaneous Reduction reaction proceeds via syn addition of hydrogen for double bonds.
Similarity Belongs to the geranylgeranyl reductase family. DGGGPL reductase subfamily.

Keywords [?]

FAD
Flavoprotein
Lipid biosynthesis
Lipid metabolism
case <OC:Thermoplasmata>
NAD
NADP
end case
Oxidoreductase
Phospholipid biosynthesis
Phospholipid metabolism

Gene Ontology [?]

case <OC:Thermoplasmata>
GO:0016628; Molecular function: oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor.
else case <OC:Methanomicrobia>
GO:0016636; Molecular function: oxidoreductase activity, acting on the CH-CH group of donors, iron-sulfur protein as acceptor.
else
GO:0016627; Molecular function: oxidoreductase activity, acting on the CH-CH group of donors.
end case
GO:0050660; Molecular function: flavin adenine dinucleotide binding.
case <OC:Thermoplasmata>
GO:0050661; Molecular function: NADP binding.
GO:0051287; Molecular function: NAD binding.
end case
GO:0008654; Biological process: phospholipid biosynthetic process.

Cross-references [?]

PRINTS PR00368; FADPNR; 1;
NCBIfam TIGR02032; GG-red-SF; 1;

Features [?]

From: GGR_THEAC (Q9HKS9)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     10     14       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     G-[AGS]-[GR]-[PVI]-[AGS]  
BINDING     33     35       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     [ED]-[KR]-x  
BINDING     44     47       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     C-[AG]-[EG]-[GA]  
BINDING     295     296       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     G-[ILM]  
BINDING     208     214       /ligand="substrate     G-Y-[VILAF]-[WL]-x-[FI]-P  
BINDING     291     294       /ligand="substrate     [ILFVM]-[TSEH]-G-G  
BINDING     100     100       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     R  
BINDING     283     283       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     D  

Additional information [?]

Size range 375-408 amino acids
Related rules None
Fusion None
Comments The protein from Thermoplasma acidophilum (Q9HKS9) was shown to be able to use both NADH and NADPH as electron donors, whereas the protein from Archaeoglobus fulgidus (O29786) cannot use NADPH as an electron donor (it can use sodium dithionite in vitro). In Methanosarcina acetivorans, a colocalized ferredoxin is the physiological electron donor. In GGR from Sulfolobus acidocaldarius, the physiological reducing agent is also unknown.


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