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Annotation rule MF_01287
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General rule information [?]

Accession MF_01287
Dates 20-AUG-2008 (Created)
12-JUN-2020 (Last updated, Version 17)
Name DGGGPL_reductase
Scope
Archaea
Templates O29786 (GGR_ARCFU); Q9HKS9 (GGR_THEAC): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
GGR
case <OC:Thermoplasmata>
Protein name
RecName: Full=Digeranylgeranylglycerophospholipid reductase;
Short=DGGGPL reductase;
EC 1.3.1.101;
AltName: Full=2,3-bis-O-geranylgeranylglyceryl phosphate reductase;
AltName: Full=Geranylgeranyl reductase;
Short=GGR;
else case <OC:Methanomicrobia>
Protein name
RecName: Full=Digeranylgeranylglycerophospholipid reductase;
Short=DGGGPL reductase;
EC 1.3.7.11;
AltName: Full=2,3-bis-O-geranylgeranylglyceryl phosphate reductase;
AltName: Full=Geranylgeranyl reductase;
Short=GGR;
else
Protein name
RecName: Full=Digeranylgeranylglycerophospholipid reductase;
Short=DGGGPL reductase;
EC 1.3.-.-;
AltName: Full=2,3-bis-O-geranylgeranylglyceryl phosphate reductase;
AltName: Full=Geranylgeranyl reductase;
Short=GGR;
end case

Comments [?]

Function Is involved in the reduction of 2,3-digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3-diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids. Catalyzes the formation of archaetidic acid (2,3-di-O-phytanyl-sn-glyceryl phosphate) from 2,3-di-O-geranylgeranylglyceryl phosphate (DGGGP) via the hydrogenation of each double bond of the isoprenoid chains.
case <OC:Thermoplasmata>
Catalytic activity RHEA:36035: 2,3-bis-(O-phytanyl)-sn-glycerol 1-phosphate + 8 NADP(+) = 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate + 8 H(+) + 8 NADPH
EC 1.3.1.101
PhysiologicalDirection=right-to-left (RHEA:36037)
RHEA:36039: 2,3-bis-(O-phytanyl)-sn-glycerol 1-phosphate + 8 NAD(+) = 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate + 8 H(+) + 8 NADH
EC 1.3.1.101
PhysiologicalDirection=right-to-left (RHEA:36041)
else case <OC:Methanomicrobia>
Catalytic activity RHEA:54324: a 2,3-bis-O-phytanyl-sn-glycerol 1-phospholipid + 8 oxidized 2[4Fe-4S]-[ferredoxin] = a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid + 16 H(+) + 8 reduced 2[4Fe-4S]-[ferredoxin]
EC 1.3.7.11
PhysiologicalDirection=right-to-left (RHEA:54326)
RHEA:36159: 2,3-bis-(O-phytanyl)-sn-glycerol 1-phosphate + 8 oxidized 2[4Fe-4S]-[ferredoxin] = 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate + 16 H(+) + 8 reduced 2[4Fe-4S]-[ferredoxin]
EC 1.3.7.11
PhysiologicalDirection=right-to-left (RHEA:36161)
else
Catalytic activity RHEA:64376: 8 A + a 2,3-bis-O-phytanyl-sn-glycerol 1-phospholipid = a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid + 8 AH2
PhysiologicalDirection=right-to-left (RHEA:64378)
RHEA:64368: 2,3-bis-(O-phytanyl)-sn-glycerol 1-phosphate + 8 A = 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate + 8 AH2
PhysiologicalDirection=right-to-left (RHEA:64370)
end case
Cofactor FAD
Note: Binds 1 FAD per subunit.
Pathway Membrane lipid metabolism; glycerophospholipid metabolism.
Miscellaneous Reduction reaction proceeds via syn addition of hydrogen for double bonds.
Similarity Belongs to the geranylgeranyl reductase family. DGGGPL reductase subfamily.

Keywords [?]

case <OC:Thermoplasmata>
end case

Gene Ontology [?]

case <OC:Thermoplasmata>
GO:0016628; Molecular function: oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor.
else case <OC:Methanomicrobia>
GO:0016636; Molecular function: oxidoreductase activity, acting on the CH-CH group of donors, iron-sulfur protein as acceptor.
else
GO:0016627; Molecular function: oxidoreductase activity, acting on the CH-CH group of donors.
end case
GO:0050660; Molecular function: flavin adenine dinucleotide binding.
case <OC:Thermoplasmata>
GO:0050661; Molecular function: NADP binding.
GO:0051287; Molecular function: NAD binding.
end case
GO:0008654; Biological process: phospholipid biosynthetic process.

Cross-references [?]

PRINTS PR00368; FADPNR; 1;
TIGRFAMs TIGR02032; GG-red-SF; 1;

Features [?]

From: GGR_THEAC (Q9HKS9)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     10     14       FAD     G-[AGS]-[GR]-[PVI]-[AGS]  
NP_BIND     33     35       FAD     [ED]-[KR]-x  
NP_BIND     44     47       FAD     C-[AG]-[EG]-[GA]  
NP_BIND     295     296       FAD     G-[ILM]  
REGION     208     214       Substrate binding     G-Y-[VILAF]-[WL]-x-[FI]-P  
REGION     291     294       Substrate binding     [ILFVM]-[TSEH]-G-G  
BINDING     100     100       FAD     R  
BINDING     283     283       FAD     D  

Additional information [?]

Size range 375-408 amino acids
Related rules None
Fusion None
Comments The protein from Thermoplasma acidophilum (Q9HKS9) was shown to be able to use both NADH and NADPH as electron donors, whereas the protein from Archaeoglobus fulgidus (O29786) cannot use NADPH as an electron donor (it can use sodium dithionite in vitro). In Methanosarcina acetivorans, a colocalized ferredoxin is the physiological electron donor. In GGR from Sulfolobus acidocaldarius, the physiological reducing agent is also unknown.