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HAMAP rule MF_01287

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General rule information [?]

Accession MF_01287
Dates 20-AUG-2008 (Created)
1-JUN-2023 (Last updated, Version 22)
Name DGGGPL_reductase
Scope(s) Archaea
Template(s) O29786 (GGR_ARCFU); Q9HKS9 (GGR_THEAC); [ Recover all ]
Triggered by HAMAP; MF_01287 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier GGR
case <OC:Thermoplasmata>
Protein name RecName: Full=Digeranylgeranylglycerophospholipid reductase;
                 Short=DGGGPL reductase;
                 EC=1.3.1.101;
AltName: Full=2,3-bis-O-geranylgeranylglyceryl phosphate reductase;
AltName: Full=Geranylgeranyl reductase;
                 Short=GGR;
else case <OC:Methanomicrobia>
Protein name RecName: Full=Digeranylgeranylglycerophospholipid reductase;
                 Short=DGGGPL reductase;
                 EC=1.3.7.11;
AltName: Full=2,3-bis-O-geranylgeranylglyceryl phosphate reductase;
AltName: Full=Geranylgeranyl reductase;
                 Short=GGR;
else
Protein name RecName: Full=Digeranylgeranylglycerophospholipid reductase;
                 Short=DGGGPL reductase;
                 EC=1.3.-.-;
AltName: Full=2,3-bis-O-geranylgeranylglyceryl phosphate reductase;
AltName: Full=Geranylgeranyl reductase;
                 Short=GGR;
end case

Comments [?]

FUNCTIONIs involved in the reduction of 2,3- digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3- diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids. Catalyzes the formation of archaetidic acid (2,3-di-O-phytanyl-sn-glyceryl phosphate) from 2,3-di- O-geranylgeranylglyceryl phosphate (DGGGP) via the hydrogenation of each double bond of the isoprenoid chains.
case <OC:Thermoplasmata>
CATALYTIC ACTIVITY Reaction=2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 NADP(+) = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 H(+) + 8 NADPH; Xref=Rhea:RHEA:36035, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:58837, ChEBI:CHEBI:73125; EC=1.3.1.101; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:36037;
CATALYTIC ACTIVITY Reaction=2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 NAD(+) = 2,3- bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 H(+) + 8 NADH; Xref=Rhea:RHEA:36039, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:58837, ChEBI:CHEBI:73125; EC=1.3.1.101; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:36041;
else case <OC:Methanomicrobia>
CATALYTIC ACTIVITY Reaction=a 2,3-bis-O-phytanyl-sn-glycerol 1-phospholipid + 8 oxidized 2[4Fe-4S]-[ferredoxin] = a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1- phospholipid + 16 H(+) + 8 reduced 2[4Fe-4S]-[ferredoxin]; Xref=Rhea:RHEA:54324, Rhea:RHEA-COMP:10002, Rhea:RHEA-COMP:10004, ChEBI:CHEBI:15378, ChEBI:CHEBI:33722, ChEBI:CHEBI:33723, ChEBI:CHEBI:138139, ChEBI:CHEBI:138140; EC=1.3.7.11; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:54326;
CATALYTIC ACTIVITY Reaction=2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 oxidized 2[4Fe-4S]-[ferredoxin] = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1- phosphate + 16 H(+) + 8 reduced 2[4Fe-4S]-[ferredoxin]; Xref=Rhea:RHEA:36159, Rhea:RHEA-COMP:10002, Rhea:RHEA-COMP:10004, ChEBI:CHEBI:15378, ChEBI:CHEBI:33722, ChEBI:CHEBI:33723, ChEBI:CHEBI:58837, ChEBI:CHEBI:73125; EC=1.3.7.11; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:36161;
else
CATALYTIC ACTIVITY Reaction=8 A + a 2,3-bis-O-phytanyl-sn-glycerol 1-phospholipid = a 2,3- bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid + 8 AH2; Xref=Rhea:RHEA:64376, ChEBI:CHEBI:13193, ChEBI:CHEBI:17499, ChEBI:CHEBI:138139, ChEBI:CHEBI:138140; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:64378;
CATALYTIC ACTIVITY Reaction=2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 A = 2,3-bis- O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 AH2; Xref=Rhea:RHEA:64368, ChEBI:CHEBI:13193, ChEBI:CHEBI:17499, ChEBI:CHEBI:58837, ChEBI:CHEBI:73125; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:64370;
end case
COFACTOR Name=FAD; Xref=ChEBI:CHEBI:57692; Note=Binds 1 FAD per subunit.;
PATHWAYMembrane lipid metabolism; glycerophospholipid metabolism.
MISCELLANEOUSReduction reaction proceeds via syn addition of hydrogen for double bonds.
SIMILARITYBelongs to the geranylgeranyl reductase family. DGGGPL reductase subfamily.

Keywords [?]

FAD
Flavoprotein
Lipid biosynthesis
Lipid metabolism
case <OC:Thermoplasmata>
NAD
NADP
end case
Oxidoreductase
Phospholipid biosynthesis
Phospholipid metabolism

Gene Ontology [?]

case <OCellular component:Thermoplasmata>
GO:0016628; Molecular function:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
else case <OCellular component:Methanomicrobia>
GO:0016636; Molecular function:oxidoreductase activity, acting on the CH-CH group of donors, iron-sulfur protein as acceptor
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0016627; Molecular function:oxidoreductase activity, acting on the CH-CH group of donors
end case
GO:0050660; Molecular function:flavin adenine dinucleotide binding
case <OCellular component:Thermoplasmata>
GO:0050661; Molecular function:NADP binding
GO:0051287; Molecular function:NAD binding
end case
GO:0008654; Biological process:phospholipid biosynthetic process

Cross-references [?]

PRINTS PR00368; FADPNR; 1;
NCBIfam TIGR02032; GG-red-SF; 1;

Features [?]

From: GGR_THEAC (Q9HKS9)
Key From To Description Tag Condition FTGroup
BINDING 10 14 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"		 G-[AGS]-[GR]-[PVI]-[AGS]
BINDING 33 35 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"		 [ED]-[KR]-x
BINDING 44 47 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"		 C-[AG]-[EG]-[GA]
BINDING 295 296 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"		 G-[ILM]
BINDING 208 214 /ligand="substrate" G-Y-[VILAF]-[WL]-x-[FI]-P
BINDING 291 294 /ligand="substrate" [ILFVM]-[TSEH]-G-G
BINDING 100 100 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"		 R
BINDING 283 283 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"		 D

Additional information [?]

Size range 375-408 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments The protein from Thermoplasma acidophilum (Q9HKS9) was shown to be able to use both NADH and NADPH as electron donors, whereas the protein from Archaeoglobus fulgidus (O29786) cannot use NADPH as an electron donor (it can use sodium dithionite in vitro). In Methanosarcina acetivorans, a colocalized ferredoxin is the physiological electron donor. In GGR from Sulfolobus acidocaldarius, the physiological reducing agent is also unknown.



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