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HAMAP rule MF_01288

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General rule information [?]

Accession MF_01288
Dates 19-SEP-2008 (Created)
26-JAN-2023 (Last updated, Version 14)
Name Rhamnon_dehydrat
Scope(s) Bacteria
Template(s) Q8ZNF9 (RHMD_SALTY); P77215 (RHMD_ECOLI); Q12DF1 (RHMD_POLSJ); [ Recover all ]
Triggered by HAMAP; MF_01288 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RHMD
case <OC:Bacteria>
Protein name RecName: Full=L-rhamnonate dehydratase;
end case
case <OC:Archaea>
Protein name RecName: Full=Putative L-rhamnonate dehydratase;
end case
Gene name Name=rhmD;

Comments [?]

FUNCTIONCatalyzes the dehydration of L-rhamnonate to 2-keto-3-deoxy- L-rhamnonate (KDR).
CATALYTIC ACTIVITY Reaction=L-rhamnonate = 2-dehydro-3-deoxy-L-rhamnonate + H2O; Xref=Rhea:RHEA:23080, ChEBI:CHEBI:15377, ChEBI:CHEBI:58118, ChEBI:CHEBI:58371; EC=;
case <FTGroup:1>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
end case
case <OC:Pseudomonadota>
SUBUNITHomooctamer; tetramer of dimers.
end case
MISCELLANEOUSReaction proceeds via a syn dehydration.
SIMILARITYBelongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0000287; Molecular function:magnesium ion binding
end case
GO:0050032; Molecular function:L-rhamnonate dehydratase activity

Cross-references [?]

PROSITE PS00908; MR_MLE_1; 1;
PROSITE PS00909; MR_MLE_2; 1;
Pfam PF01188; MR_MLE; 1;
Pfam PF02746; MR_MLE_N; 1;

Features [?]

Key From To Description Tag Condition FTGroup
ACT_SITE 329 329 /note="Proton acceptor" H
BINDING 226 226 /ligand="Mg(2+)"
D 1
BINDING 252 252 /ligand="Mg(2+)"
E 1
BINDING 280 280 /ligand="Mg(2+)"
E 1
BINDING 33 33 /ligand="substrate" H
BINDING 59 59 /ligand="substrate" R
BINDING 349 349 /ligand="substrate" E
SITE 302 302 /note="Increases basicity of active site His" D
SITE 349 349 /note="Transition state stabilizer" E

Additional information [?]

Size range 390-428 amino acids
Related rules None
Fusion Nter: None Cter: None

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