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HAMAP rule MF_01291

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General rule information [?]

Accession MF_01291
Dates 3-OCT-2008 (Created)
1-JUN-2023 (Last updated, Version 17)
Name KDGluc_aldolase
Scope(s) Bacteria
Template(s) P23522 (GARL_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01291 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier GARL
Protein name RecName: Full=5-keto-4-deoxy-D-glucarate aldolase;
                 Short=KDGluc aldolase;
AltName: Full=2-dehydro-3-deoxy-D-glucarate aldolase;
AltName: Full=2-keto-3-deoxy-D-glucarate aldolase;
AltName: Full=5-dehydro-4-deoxy-D-glucarate aldolase;
AltName: Full=Alpha-keto-beta-deoxy-D-glucarate aldolase;
Gene name Name=garL;

Comments [?]

FUNCTIONCatalyzes the reversible retro-aldol cleavage of both 5-keto- 4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde.
CATALYTIC ACTIVITY Reaction=5-dehydro-4-deoxy-D-glucarate = 2-hydroxy-3-oxopropanoate + pyruvate; Xref=Rhea:RHEA:27726, ChEBI:CHEBI:15361, ChEBI:CHEBI:42819, ChEBI:CHEBI:57978;
CATALYTIC ACTIVITY Reaction=2-dehydro-3-deoxy-D-glucarate = 2-hydroxy-3-oxopropanoate + pyruvate; Xref=Rhea:RHEA:10268, ChEBI:CHEBI:15361, ChEBI:CHEBI:57978, ChEBI:CHEBI:58098; EC=;
case <FTGroup:1>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
end case
PATHWAYCarbohydrate acid metabolism; galactarate degradation; D- glycerate from galactarate: step 2/3.
SUBUNITHomohexamer; trimer of dimers.
SIMILARITYBelongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0000287; Molecular function:magnesium ion binding
end case
GO:0008672; Molecular function:2-dehydro-3-deoxyglucarate aldolase activity
GO:0042838; Biological process:D-glucarate catabolic process
GO:0046392; Biological process:galactarate catabolic process

Cross-references [?]

Pfam PF03328; HpcH_HpaI; 1;
NCBIfam TIGR03239; GarL; 1;

Features [?]

From: GARL_ECOLI (P23522)
Key From To Description Tag Condition FTGroup
ACT_SITE 50 50 /note="Proton acceptor" H
BINDING 153 153 /ligand="Mg(2+)"
E 1
BINDING 179 179 /ligand="Mg(2+)"
D 1
BINDING 151 151 /ligand="substrate" Q
BINDING 178 178 /ligand="substrate" S
BINDING 179 179 /ligand="substrate" D
SITE 75 75 /note="Transition state stabilizer" R
SITE 89 89 /note="Increases basicity of active site His" D

Additional information [?]

Size range 250-282 amino acids
Related rules None
Fusion Nter: None Cter: None

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