HAMAP rule MF_01291
General rule information
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| Accession | MF_01291 |
| Dates | 3-OCT-2008 (Created)
1-JUN-2023 (Last updated, Version 17) |
| Name | KDGluc_aldolase |
| Scope(s) |
Bacteria Gammaproteobacteria |
| Template(s) | P23522 (GARL_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01291 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | GARL |
| Protein name | RecName: Full=5-keto-4-deoxy-D-glucarate aldolase; Short=KDGluc aldolase; Short=KDGlucA; EC=4.1.2.20; AltName: Full=2-dehydro-3-deoxy-D-glucarate aldolase; AltName: Full=2-keto-3-deoxy-D-glucarate aldolase; AltName: Full=5-dehydro-4-deoxy-D-glucarate aldolase; AltName: Full=Alpha-keto-beta-deoxy-D-glucarate aldolase; |
| Gene name | Name=garL; |
Comments
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| FUNCTION | Catalyzes the reversible retro-aldol cleavage of both 5-keto- 4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde. |
| CATALYTIC ACTIVITY | Reaction=5-dehydro-4-deoxy-D-glucarate = 2-hydroxy-3-oxopropanoate + pyruvate; Xref=Rhea:RHEA:27726, ChEBI:CHEBI:15361, ChEBI:CHEBI:42819, ChEBI:CHEBI:57978; |
| CATALYTIC ACTIVITY | Reaction=2-dehydro-3-deoxy-D-glucarate = 2-hydroxy-3-oxopropanoate + pyruvate; Xref=Rhea:RHEA:10268, ChEBI:CHEBI:15361, ChEBI:CHEBI:57978, ChEBI:CHEBI:58098; EC=4.1.2.20; |
| case <FTGroup:1> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
| end case | |
| PATHWAY | Carbohydrate acid metabolism; galactarate degradation; D- glycerate from galactarate: step 2/3. |
| SUBUNIT | Homohexamer; trimer of dimers. |
| SIMILARITY | Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily. |
Keywords
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| Lyase | |
| case <FTGroup:1> | |
| Magnesium | |
| Metal-binding | |
| end case | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
| GO:0008672; Molecular function:2-dehydro-3-deoxyglucarate aldolase activity | |
| GO:0042838; Biological process:D-glucarate catabolic process | |
| GO:0046392; Biological process:galactarate catabolic process | |
Cross-references
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Features
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| From: GARL_ECOLI (P23522) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 50 | 50 | /note="Proton acceptor" | H | ||||||||
| BINDING | 153 | 153 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | 1 | |||||||
| BINDING | 179 | 179 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 151 | 151 | /ligand="substrate" | Q | ||||||||
| BINDING | 178 | 178 | /ligand="substrate" | S | ||||||||
| BINDING | 179 | 179 | /ligand="substrate" | D | ||||||||
| SITE | 75 | 75 | /note="Transition state stabilizer" | R | ||||||||
| SITE | 89 | 89 | /note="Increases basicity of active site His" | D | ||||||||
Additional information
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| Size range | 250-282 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |