HAMAP rule MF_01312

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_01312
Accession	MF_01312
Dates	28-FEB-2005 (Created) 19-NOV-2022 (Last updated, Version 34)
Name	NorV
Scope(s)	Bacteria Aeromonas Aliivibrio Citrobacter Escherichia Enterobacter Klebsiella Pectobacterium Salmonella Serratia Shigella Vibrio
Template(s)	Q46877; [ Recover all ]
Triggered by	HAMAP; MF_01312 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	NORV
Protein name	RecName: Full=Anaerobic nitric oxide reductase flavorubredoxin; Short=FlRd; Short=FlavoRb;
Gene name	Name=norV; Synonyms=flrD;

Comments [?]

FUNCTION	Anaerobic nitric oxide reductase; uses NADH to detoxify nitric oxide (NO), protecting several 4Fe-4S NO-sensitive enzymes. Has at least 2 reductase partners, only one of which (NorW, flavorubredoxin reductase) has been identified. NO probably binds to the di-iron center; electrons enter from the NorW at rubredoxin and are transferred sequentially to the FMN center and the di-iron center. Also able to function as an aerobic oxygen reductase.
case <FTGroup:1> and <FTGroup:2>
COFACTOR	Name=Fe cation; Xref=ChEBI:CHEBI:24875; Note=Binds 3 Fe cations per monomer.;
end case
case <Feature:PS50902>
COFACTOR	Name=FMN; Xref=ChEBI:CHEBI:58210; Note=Binds 1 FMN per monomer.;
end case
PATHWAY	Nitrogen metabolism; nitric oxide reduction.
SUBUNIT	Homotetramer.
SUBCELLULAR LOCATION	Cytoplasm.
case <FT:1>
SIMILARITY	In the N-terminal section; belongs to the zinc metallo- hydrolase group 3 family.
end case

Keywords [?]

Gene Ontology [?]

GO:0009055; Molecular function:electron transfer activity

GO:0016491; Molecular function:oxidoreductase activity

GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Features [?]

From: NORV_ECOLI (Q46877)
Key	From	To	Description	Tag	Condition	FTGroup
DOMAIN	423	474	/note="Rubredoxin-like"
REGION	30	210	/note="Zinc metallo-hydrolase"
BINDING	79	79	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1"		H	1
BINDING	81	81	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1"		E	1
BINDING	83	83	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2"		D	1
BINDING	147	147	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1"		H	1
BINDING	166	166	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1"		D	1
BINDING	166	166	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2"		D	1
BINDING	227	227	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2"		H	1
BINDING	428	428	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="3"		C	2
BINDING	431	431	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="3"		C	2
BINDING	461	461	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="3"		C	2
BINDING	464	464	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="3"		C	2

Additional information [?]

Size range	479-521 amino acids
Related rules	None
Fusion	Nter: None Cter: None
Comments	ECO57 lacks the flavodoxin-like domain and is not used in alignment. Restricted to lineages where there is norR upstream and norW downstream.