HAMAP rule MF_01312
General rule information
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| Accession | MF_01312 |
| Dates | 20-MAR-2003 (Created)
19-NOV-2022 (Last updated, Version 38) |
| Name | NorV |
| Scope(s) |
Bacteria Aeromonas Aliivibrio Citrobacter Escherichia Enterobacter Klebsiella Pectobacterium Salmonella Serratia Shigella Vibrio |
| Template(s) | Q46877 (NORV_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01312 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | NORV |
| Protein name | RecName: Full=Anaerobic nitric oxide reductase flavorubredoxin; Short=FlRd; Short=FlavoRb; |
| Gene name | Name=norV; Synonyms=flrD; |
Comments
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| FUNCTION | Anaerobic nitric oxide reductase; uses NADH to detoxify nitric oxide (NO), protecting several 4Fe-4S NO-sensitive enzymes. Has at least 2 reductase partners, only one of which (NorW, flavorubredoxin reductase) has been identified. NO probably binds to the di-iron center; electrons enter from the NorW at rubredoxin and are transferred sequentially to the FMN center and the di-iron center. Also able to function as an aerobic oxygen reductase. |
| case <FTGroup:1> and <FTGroup:2> | |
| COFACTOR | Name=Fe cation; Xref=ChEBI:CHEBI:24875; Note=Binds 3 Fe cations per monomer.; |
| end case | |
| case <Feature:PS50902> | |
| COFACTOR | Name=FMN; Xref=ChEBI:CHEBI:58210; Note=Binds 1 FMN per monomer.; |
| end case | |
| PATHWAY | Nitrogen metabolism; nitric oxide reduction. |
| SUBUNIT | Homotetramer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| case <FT:1> | |
| SIMILARITY | In the N-terminal section; belongs to the zinc metallo- hydrolase group 3 family. |
| end case | |
Keywords
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| Cytoplasm | |
| Transport | |
| Electron transport | |
| Oxidoreductase | |
| case <Feature:PS50902> | |
| Flavoprotein | |
| FMN | |
| end case | |
| case <FTGroup:1> or <FTGroup:2> | |
| Metal-binding | |
| Iron | |
| end case | |
Gene Ontology
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| GO:0009055; Molecular function:electron transfer activity |
| GO:0016491; Molecular function:oxidoreductase activity |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF00258; Flavodoxin_1; 1; |
| Pfam | PF00753; Lactamase_B; 1; |
| Pfam | PF00301; Rubredoxin; 1; |
| Pfam | PF00070; Pyr_redox; 1; |
| PIRSF | PIRSF005243; ROO; 1; |
| PRINTS | PR00163; RUBREDOXIN; 1; |
| PROSITE | PS50902; FLAVODOXIN_LIKE; 1; |
| PROSITE | PS50903; RUBREDOXIN_LIKE; 1; |
Features
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| From: NORV_ECOLI (Q46877) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | 423 | 474 | /note="Rubredoxin-like" | |||||||||
| REGION | 30 | 210 | /note="Zinc metallo-hydrolase" | |||||||||
| BINDING | 79 | 79 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1" |
H | 1 | |||||||
| BINDING | 81 | 81 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1" |
E | 1 | |||||||
| BINDING | 83 | 83 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2" |
D | 1 | |||||||
| BINDING | 147 | 147 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1" |
H | 1 | |||||||
| BINDING | 166 | 166 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1" |
D | 1 | |||||||
| BINDING | 166 | 166 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2" |
D | 1 | |||||||
| BINDING | 227 | 227 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2" |
H | 1 | |||||||
| BINDING | 428 | 428 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="3" |
C | 2 | |||||||
| BINDING | 431 | 431 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="3" |
C | 2 | |||||||
| BINDING | 461 | 461 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="3" |
C | 2 | |||||||
| BINDING | 464 | 464 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="3" |
C | 2 | |||||||
Additional information
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| Size range | 479-521 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | ECO57 lacks the flavodoxin-like domain and is not used in alignment. Restricted to lineages where there is norR upstream and norW downstream. |