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Annotation rule MF_01322 |
General rule information
[?]
Accession |
MF_01322 |
Dates |
6-JAN-2004 (Created) 19-NOV-2019 (Last updated, Version 23) |
Scope |
Bacteria; except Cyanobacteria
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Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
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Full=DNA-directed RNA polymerase subunit beta';
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Short=RNAP subunit beta';
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EC 2.7.7.6;
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AltName:
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Full=RNA polymerase subunit beta';
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AltName:
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Full=Transcriptase subunit beta';
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|
Function |
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. |
Catalytic activity |
RHEA:21248: a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
EC 2.7.7.6
|
case <FTGroup:2>
Cofactor |
Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
end case
case <FTGroup:1> and <FTGroup:3>
Cofactor |
Zn(2+) Note: Binds 2 Zn(2+) ions per subunit. |
end case
Subunit |
The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. |
Similarity |
Belongs to the RNA polymerase beta' chain family. |
case (<OC:Escherichia> or <OC:Shigella>) and <FT:1>
end case
case <FTGroup:1> or <FTGroup:2> or <FTGroup:3>
end case
case <FTGroup:2>
end case
case <FTGroup:1> or <FTGroup:3>
end case
GO:0003677; Molecular function: DNA binding.
GO:0003899; Molecular function: DNA-directed 5'-3' RNA polymerase activity.
case <FTGroup:2>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0006351; Biological process: transcription, DNA-templated.
case <FTGroup:1> or <FTGroup:3>
end case
From: RPOC_ECOLI (P0A8T7) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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METAL
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70
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70
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Zinc 1
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|
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C
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1
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METAL
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72
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72
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Zinc 1
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C
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1
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METAL
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85
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85
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Zinc 1
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|
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C
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1
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METAL
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88
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88
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Zinc 1
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|
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C
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1
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METAL
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460
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460
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Magnesium
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D
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2
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METAL
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462
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462
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Magnesium
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D
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2
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METAL
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464
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464
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Magnesium
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D
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2
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METAL
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814
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814
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Zinc 2
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C
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3
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METAL
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888
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888
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Zinc 2
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C
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3
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METAL
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895
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895
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Zinc 2
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C
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3
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METAL
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898
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898
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Zinc 2
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C
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3
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case <OC:Escherichia> or <OC:Shigella>
MOD_RES
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983
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983
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N6-acetyllysine
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K
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end case
Additional information
[?]
Size range |
1135-1690 amino acids |
Related rules |
MF_01323 (RPOC1); MF_01324 (RPOC2) |
Fusion |
Nter: MF_01321 (rpoB), <Unknown>; Cter: None |
Comments |
Fused with rpoB in Helicobacter species and least some Wolbachia, but not in other epsilon proteobateria. In Acholeplasmataceae there is an unknown N-terminal extension of about 110 amino acids. In cyanobacteria and chloroplasts this protein is split into two parts. The N-terminus is known as gamma in cyanobacteria and beta' in chloroplasts (MF_01323), while the C-terminus is known as beta' in cyanobacteria and beta'' in chloroplasts (MF_01324). Many Mycoplasma only have 1 Zn ion-binding site. |