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HAMAP rule MF_01336

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General rule information [?]

Accession MF_01336
Dates 16-JUN-2005 (Created)
22-SEP-2023 (Last updated, Version 17)
Name Ribosomal_bL25
Scope(s) Bacteria
Template(s) P68919 (RL25_ECOLI); Q5SHZ1 (RL25_THET8); P56930 (RL25_THETH); [ Recover all ]
Triggered by HAMAP; MF_01336 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RL25
Protein name RecName: Full=Large ribosomal subunit protein bL25;
Gene name Name=rplY;

Comments [?]

FUNCTIONThis is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance.
SUBUNITPart of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA. Binds to the 5S rRNA independently of L5 and L18.
SIMILARITYBelongs to the bacterial ribosomal protein bL25 family.

Keywords [?]

Gene Ontology [?]

GO:0003723; Molecular function:RNA binding
GO:0006412; Biological process:translation

Cross-references [?]

Pfam PF01386; Ribosomal_L25p; 1;

Features [?]

Additional information [?]

Size range 92-103 amino acids
Related rules MF_01334
Fusion Nter: None Cter: None
Comments This family is specific for proteins with only the L25 domain. This protein varies in length; most bacteria encode both the L25 and CTC domains (see MF_01334). However a number of bacteria, including some Gammaproteobacteria, Cyanobacteriota and at least one Betaproteobacteria, have only the L25 domain (this family), while A. aeolicus only has the CTC domain (and cannot bind 5S rRNA). In at least some cases bL25 is dispensible for translation; in B. subtilis bL25 is only expressed in vegetatively growing cells. bL25 is missing from some Bacillus, Cyanobacteriota and Lactobacillus. The equivalent of the highly conserved Asp-87 in Thermus thermophilus (AC P56930) is altered in Enterococcus and Cyanobacteriota. There are compensatory changes in the 5S rRNA that are thought to alter its secondary structure and thus permit binding of the protein to rRNA, see: PubMed=15718233; DOI=10.1074/jbc.M413596200; Gongadze G.M., Korepanov A.P., Stolboushkina E.A., Zelinskaya N.V., Korobeinikova A.V., Ruzanov M.V., Eliseev B.D., Nikonov O.S., Nikonov S.V., Garber M.B., Lim V.I.; "The crucial role of conserved intermolecular H-bonds inaccessible to the solvent in formation and stabilization of the TL5.5 SrRNA complex."; J. Biol. Chem. 280:16151-16156(2005).

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