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HAMAP rule MF_01338

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General rule information [?]

Accession MF_01338
Dates 5-AUG-2005 (Created)
17-FEB-2023 (Last updated, Version 50)
Name RuBisCO_L_type1
Scope
Bacteria; Cyanobacteriota
Bacteria; Pseudomonadota
Bacteria; Actinomycetota
Bacteria; Verrucomicrobiota
Plastid
Templates P00876 (RBL_TOBAC); P0C2C2 (RBL1C_CUPNE); P00880 (RBL_SYNP6); P00877 (RBL_CHLRE); P0C512 (RBL_ORYSJ); P00875 (RBL_SPIOL); F4CQ77 (RBL_PSEUX); Q31NB3 (RBL_SYNE7): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
RBL
case <OG:Chloroplast> and <OC:Viridiplantae>
Protein name
RecName: Full=Ribulose bisphosphate carboxylase large chain;
Short=RuBisCO large subunit;
EC 4.1.1.39;
Flags: Precursor;
Gene name
rbcL
else case <OG:Chloroplast> and not <OC:Viridiplantae>
Protein name
RecName: Full=Ribulose bisphosphate carboxylase large chain;
Short=RuBisCO large subunit;
EC 4.1.1.39;
Gene name
rbcL
else case <OC:Cyanobacteriota>
Protein name
RecName: Full=Ribulose bisphosphate carboxylase large chain;
Short=RuBisCO large subunit;
EC 4.1.1.39;
Gene name
cbbL, rbcL
else case not <OC:Cyanobacteriota> and not <OG:Chloroplast>
Protein name
RecName: Full=Ribulose bisphosphate carboxylase large chain;
Short=RuBisCO large subunit;
EC 4.1.1.39;
Gene name
cbbL
end case

Comments [?]

case <OG:Chloroplast> or <OC:Cyanobacteriota>
Function RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.
else case not <OC:Cyanobacteriota> and not <OG:Chloroplast>
Function RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.
end case
Catalytic activity RHEA:23124: 2 (2R)-3-phosphoglycerate + 2 H(+) = CO2 + D-ribulose 1,5-bisphosphate + H2O
EC 4.1.1.39
RHEA:36631: D-ribulose 1,5-bisphosphate + O2 = (2R)-3-phosphoglycerate + 2-phosphoglycolate + 2 H(+)
case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
end case
case <FT:18>
Subunit Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers.
else
Subunit Heterohexadecamer of 8 large chains and 8 small chains.
end case
case <OC:Cyanobacteriota>
Subcellular location Carboxysome.
end case
case <OG:Chloroplast>
Subcellular location Plastid, chloroplast.
end case
case <FT:18>
Ptm The disulfide bond which can form in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover.
end case
Miscellaneous The basic functional RuBisCO is composed of a large chain homodimer in a 'head-to-tail' conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric 'cap' on each end of the 'barrel'.
Similarity Belongs to the RuBisCO large chain family. Type I subfamily.

Keywords [?]

case <FT:15>
end case
case <OC:Cyanobacteriota>
end case
case <FT:18>
end case
case <FTGroup:1>
end case
case <FT:16>
end case
case <OG:Chloroplast> or <OC:Cyanobacteriota>
end case
case <OG:Chloroplast> or <Property:PHOTOSYN>
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0015977; Biological process: carbon fixation.
GO:0016984; Molecular function: ribulose-bisphosphate carboxylase activity.
GO:0019253; Biological process: reductive pentose-phosphate cycle.
case <OC:Cyanobacteriota>
GO:0031470; Cellular component: carboxysome.
end case
case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case

Cross-references [?]

PROSITE PS00157; RUBISCO_LARGE; 1;
Pfam PF00016; RuBisCO_large; 1;
PF02788; RuBisCO_large_N; 1;

Features [?]

case <OC:Viridiplantae>
From: RBL_TOBAC (P00876)
Key     From     To       Description   Tag   Condition   FTGroup
PROPEP     1     2                
CHAIN     3     Cter       Ribulose bisphosphate carboxylase large chain        
end case
SITE     334     334       Transition state stabilizer     K  
ACT_SITE     175     175       Proton acceptor     K  
ACT_SITE     294     294       Proton acceptor     H  
BINDING     201     201       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /note="via carbamate group     K   1
BINDING     203     203       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D   1
BINDING     204     204       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     E   1
BINDING     123     123       /ligand="substrate" /note="in homodimeric partner     N  
BINDING     173     173       /ligand="substrate     T  
BINDING     177     177       /ligand="substrate     K  
BINDING     295     295       /ligand="substrate     R  
BINDING     327     327       /ligand="substrate     H  
BINDING     379     379       /ligand="substrate     S  
case <OC:Viridiplantae>
MOD_RES     3     3       N-acetylproline     P  
MOD_RES     14     14       N6,N6,N6-trimethyllysine     K  
end case
MOD_RES     201     201       N6-carboxylysine     K  
DISULFID (Optional)     247     247       Interchain; in linked form     C  

Additional information [?]

Size range 463-501 amino acids
Related rules MF_01133 (RBL); MF_01339 (RBL2)
Fusion None