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HAMAP rule MF_01356

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General rule information [?]

Accession MF_01356
Dates 17-NOV-2008 (Created)
15-JUN-2023 (Last updated, Version 32)
Name NDH1_NuoB
Scope
Bacteria
Plastid
Template Q56218 (NQO6_THET8)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

case <OG:Chloroplast>
Identifier
NDHK
Protein name
RecName: Full=NAD(P)H-quinone oxidoreductase subunit K, chloroplastic;
EC 7.1.1.-;
AltName: Full=NAD(P)H dehydrogenase subunit K;
AltName: Full=NADH-plastoquinone oxidoreductase subunit K;
Gene name
ndhK
else case <OC:Cyanobacteriota>
Identifier
NDHK
Protein name
RecName: Full=NAD(P)H-quinone oxidoreductase subunit K;
EC 7.1.1.-;
AltName: Full=NAD(P)H dehydrogenase I subunit K;
AltName: Full=NDH-1 subunit K;
Short=NDH-K;
Gene name
ndhK
else
Identifier
NUOB
Protein name
RecName: Full=NADH-quinone oxidoreductase subunit B;
EC 7.1.1.-;
AltName: Full=NADH dehydrogenase I subunit B;
AltName: Full=NDH-1 subunit B;
Gene name
nuoB
end case

Comments [?]

case <OG:Chloroplast>
Function NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient.
else case <OC:Cyanobacteriota>
Function NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient. Cyanobacterial NDH-1 also plays a role in inorganic carbon-concentration.
else case <OC:Actinomycetota> or <OC:Bacteroidota> or <OC:Chlorobiota> or <OC:Deinococcota> or <OC:Bacillota>
Function NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
else
Function NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
end case
case <OG:Chloroplast> or <OC:Cyanobacteriota>
Catalytic activity RHEA:42608: a plastoquinone + (n+1) H(+)(in) + NADH = a plastoquinol + n H(+)(out) + NAD(+)
RHEA:42612: a plastoquinone + (n+1) H(+)(in) + NADPH = a plastoquinol + n H(+)(out) + NADP(+)
else
Catalytic activity RHEA:57888: a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)
end case
case <FTGroup:1>
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster.
end case
case <OG:Chloroplast>
Subunit NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus.
else case <OC:Cyanobacteriota>
Subunit NDH-1 can be composed of about 15 different subunits; different subcomplexes with different compositions have been identified which probably have different functions.
else case <OC:Enterobacterales> or <OC:Shewanellaceae> or <OC:Pseudomonadaceae>
Subunit NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.
else case <OC:Deinococci>
Subunit NDH-1 is composed of 15 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.
else
Subunit NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.
end case
case <OG:Chloroplast>
Subcellular location Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side.
else case <OC:Cyanobacteriota> and not <OC:Gloeobacter>
Subcellular location Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side.
else case <OC:Gloeobacter>
Subcellular location Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
else case not defined <Property:Membrane> or <Property:Membrane=1>
Subcellular location Cell membrane; Peripheral membrane protein; Cytoplasmic side.
else case <Property:Membrane=2>
Subcellular location Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
end case
Similarity Belongs to the complex I 20 kDa subunit family.

Keywords [?]

case <FTGroup:1>
4Fe-4S
Iron
Iron-sulfur
Metal-binding
end case
NAD
case <OG:Chloroplast> or <OC:Cyanobacteriota>
NADP
Plastoquinone
else case not <OC:Actinomycetota> and not <OC:Bacteroidota> and not <OC:Chlorobiota> and not <OC:Deinococcota> and not <OC:Bacillota>
Ubiquinone
end case
Quinone
Membrane
Translocase
Transport
case <OG:Chloroplast> or <OC:Cyanobacteriota> and not <OC:Gloeobacter>
Thylakoid
else case <OC:Gloeobacter>
Cell membrane
Cell inner membrane
else case not defined <Property:Membrane> or <Property:Membrane=1>
Cell membrane
else case <Property:Membrane=2>
Cell membrane
Cell inner membrane
end case

Gene Ontology [?]

GO:0005506; Molecular function: iron ion binding.
case <OG:Chloroplast> or <OC:Cyanobacteriota>
GO:0016655; Molecular function: oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor.
GO:0019684; Biological process: photosynthesis, light reaction.
else
GO:0050136; Molecular function: NADH dehydrogenase (quinone) activity.
end case
case <OC:Cyanobacteriota> and not <OC:Gloeobacter>
GO:0042651; Cellular component: thylakoid membrane.
else case <OG:Chloroplast>
GO:0009535; Cellular component: chloroplast thylakoid membrane.
else
GO:0005886; Cellular component: plasma membrane.
end case

Cross-references [?]

PROSITE PS01150; COMPLEX1_20K; 1;
Pfam PF01058; Oxidored_q6; 1;
NCBIfam TIGR01957; NuoB_fam; 1;

Features [?]

From: NQO6_THET8 (Q56218)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     45     45       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883     C   1
BINDING     46     46       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883     C   1
BINDING     111     111       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883     C   1
BINDING     140     140       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883     C   1

Additional information [?]

Size range 158-281 amino acids
Related rules None
Fusion Nter: None; Cter: MF_01359 (nuoCD)
Comments 14 proteins form the NDH-1 complex in most bacteria, there are at least 15 subunits in cyanobacteria and chloroplasts. PARDE and THET8 are annotated with another nomenclature. In the Geobacter there is also a fusion with nuoCD.


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