HAMAP rule MF_01375

General rule information [?]

Accession	MF_01375
Dates	29-MAR-2007 (Created) 26-JAN-2023 (Last updated, Version 24)

Name

PhnX

Scope

Bacteria; Bacteroidota

Bacteria; Bacillota

Bacteria; Pseudomonadota

Templates

O31156 (PHNX_BACCE); Q7ZAP3 (PHNX_SALTY): [Recover all]

Triggered by

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PHNX

Protein name

RecName:		Full=Phosphonoacetaldehyde hydrolase;
		Short=Phosphonatase;
		EC 3.11.1.1;
AltName:		Full=Phosphonoacetaldehyde phosphonohydrolase;

Gene name

phnX

Comments [?]

Function	Involved in phosphonate degradation.
Catalytic activity	RHEA:18905: H2O + phosphonoacetaldehyde = acetaldehyde + H(+) + phosphate EC 3.11.1.1
Cofactor	Mg(2+) Note: Binds 1 Mg(2+) ion per subunit.
Subunit	Homodimer.
Similarity	Belongs to the HAD-like hydrolase superfamily. PhnX family.

Keywords [?]

case <FT:3> or <FT:4> or <FT:5>

end case

case <FT:2>

end case

Gene Ontology [?]

GO:0050194; Molecular function: phosphonoacetaldehyde hydrolase activity.

case <FT:3> or <FT:4> or <FT:5>

GO:0000287; Molecular function: magnesium ion binding.

end case

Cross-references [?]

Features [?]

From: PHNX_BACCE (O31156)

Key From To Description Tag Condition FTGroup

ACT_SITE 9 9 Nucleophile D

ACT_SITE 50 50 Schiff-base intermediate with substrate K

BINDING 9 9 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D

BINDING 11 11 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 A

BINDING 183 183 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D

Additional information [?]

Size range	263-295 amino acids
Related rules	None
Fusion	Nter: None; Cter: MF_01376 (phnW)
Comments	In PHNXL_SYNFM (A0LJ16), the conserved Lys that has been shown to form a Schiff base with the substrate is an Arg, a mutation which completely inactivates the enzyme in Bacillus cereus. This entry is therefore annotated as atypical. Fused with 2-aminoethylphosphonate--pyruvate transaminase (phnW) in CLOD6. For a discussion of phosphonate degradation pathways see: PubMed=16245012; DOI=10.1007/s00239-004-0349-4; Huang J., Su Z., Xu Y.; "The evolution of microbial phosphonate degradative pathways."; J. Mol. Evol. 61:682-690(2005).