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Annotation rule MF_01408
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General rule information [?]

Accession MF_01408
Dates 17-OCT-2002 (Created)
19-NOV-2019 (Last updated, Version 27)
Name ThyX
Scope
Bacteria
Archaea
Templates O26061 (THYX_HELPY); Q9WYT0 (THYX_THEMA); P9WG57 (THYX_MYCTU): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
THYX
Protein name
RecName: Full=Flavin-dependent thymidylate synthase;
Short=FDTS;
EC 2.1.1.148;
AltName: Full=FAD-dependent thymidylate synthase;
AltName: Full=Thymidylate synthase ThyX;
Short=TS;
Short=TSase;
Gene name
thyX

Comments [?]

Function Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant.
Catalytic activity RHEA:29043: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP + H(+) + NADPH = (6S)-5,6,7,8-tetrahydrofolate + dTMP + NADP(+)
EC 2.1.1.148
Cofactor FAD
Note: Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.
Pathway Pyrimidine metabolism; dTTP biosynthesis.
Subunit Homotetramer.
Similarity Belongs to the thymidylate synthase ThyX family.

Keywords [?]

Transferase
Methyltransferase
Nucleotide biosynthesis
FAD
Flavoprotein
NADP

Gene Ontology [?]

GO:0050797; Molecular function: thymidylate synthase (FAD) activity.
GO:0006231; Biological process: dTMP biosynthetic process.

Cross-references [?]

Pfam PF02511; Thy1; 1;
TIGRFAMs TIGR02170; ThyX; 1;
PROSITE PS51331; THYX; 1; trigger=PRU00661;

Features [?]

From: THYX_THEMA (Q9WYT0)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     78     80       FAD     R-x-R  
NP_BIND     163     165       FAD; shared with neighboring subunits     [ND]-x-[RHN]  
NP_BIND (Optional)     75     78       dUMP; shared with dimeric partner     [QE]-x-x-R  
NP_BIND     86     90       dUMP     [EQ]-x-S-x-R  
case not <FT:4>
NP_BIND     88     90       dUMP     S-x-R  
end case
ACT_SITE     174     174       Involved in ionization of N3 of dUMP, leading to its activation     R  
BINDING (Optional)     55     55       FAD; shared with neighboring subunits     [TS]  
BINDING (Optional)     86     86       FAD; shared with neighboring subunits     [EQ]  
BINDING (Optional)     169     169       FAD     [NH]  
BINDING     147     147       dUMP     [RKH]  
BINDING     174     174       dUMP; shared with dimeric partner     R  

Additional information [?]

Size range 204-305 amino acids
Related rules None
Fusion None
Comments Divergent AQUAE and AERPE not shown in alignment and not used in size range


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