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Annotation rule MF_01408 |
Accession | MF_01408 |
Dates | 17-OCT-2002 (Created) 19-NOV-2019 (Last updated, Version 27) |
Name | ThyX |
Scope | Bacteria
Archaea |
Templates | O26061 (THYX_HELPY); Q9WYT0 (THYX_THEMA); P9WG57 (THYX_MYCTU): [Recover all] |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant. |
Catalytic activity | RHEA:29043: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP + H(+) + NADPH = (6S)-5,6,7,8-tetrahydrofolate + dTMP + NADP(+)
EC 2.1.1.148 |
Cofactor | FAD Note: Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers. |
Pathway | Pyrimidine metabolism; dTTP biosynthesis. |
Subunit | Homotetramer. |
Similarity | Belongs to the thymidylate synthase ThyX family. |
From: THYX_THEMA (Q9WYT0) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
NP_BIND | 78 | 80 | FAD | R-x-R | ||||||||
NP_BIND | 163 | 165 | FAD; shared with neighboring subunits | [ND]-x-[RHN] | ||||||||
NP_BIND (Optional) | 75 | 78 | dUMP; shared with dimeric partner | [QE]-x-x-R | ||||||||
NP_BIND | 86 | 90 | dUMP | [EQ]-x-S-x-R |
Size range | 204-305 amino acids |
Related rules | None |
Fusion | None |
Comments | Divergent AQUAE and AERPE not shown in alignment and not used in size range |